New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 3920892	46 (0.670)	3 (0.050)	3/318	NT	5.9%	coding (874/1689 nt)	acs	Butyrate‑‑CoA ligase
	?	AE015451	= 3920902	53 (0.820)					coding (864/1689 nt)	acs	Butyrate‑‑CoA ligase

ACGCGACGCTCCAGGTCGTCAGCGGCACCGGTCTGCAAGGACACCGGGCTGGTCTCGGTCATGCCATAGGCAATCTGCACTTCGGCCATGTGCATCTCGCCAATCACCCGGCGCATCACCTCGATCGGGCAGGTCGCACCGGCCATGATCCCGGTGCGCAGGCTCGACAGGTCGAATTCACCACGCTGTGGATGGTCCAGTTCGGCAATGAACATGGTCGGCACGCCATACAGCGCAGTGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/3920651‑3920892 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcgcagtggcGCAGGAAAAAGCCACTGCGCTGTATGGCGTGCCGACCATGTTCATTGCCGAACTGGACCATCCACAGCGTGGTGAATTCGACCTGTCGAGCCTGCGCACCGGGATCATGGCCGGTGCGACCTGCCCGATCGAGGTGATGCGCCGGGTGATTGGCGAGATGCACATGGCCGAAGTGCAGATTGCCTATGGCATGACCGAGACCAGCCCGGTGTCCTTGCAGACCGGTGCCGCTGAC  <  AE015451/3920902‑3920668                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               tCGCGACGCTCCAGGTCGTCAGCGGCACCGGTCTGCAAAGACACCGGGCTGGTCTCGGTCATGCCATAGGCAATCTGCACTTCGGCCATGTGCATCTCGCCAATCACCCGGCGCATCACCTCGATCGGGCAGGTCGCACCGGCCATGATCCCGGTGCGCAGGCTCGACAGGTCGAATTCACCACGCTGTGGATGGTCCAGTTCGGCAATGAACATGGTCGGCACGCCATACAG                                                                                                                                                                                                                                                      >  2:758602/2‑233                                                                             GCACTTCGGCCATGTGCATCTCGCCAATCACCCGGCGCATCACCTCGATCGGGCAGGTCGCACCGGCCATGATCCCGGTGCGCAGGCTCGACAGGTCGAATTCACCACGCTGTGGATGGTCCAGTTCGGCAATGAACATGGTCGGCACGCCATACAGCG                                                                                                                                                                                                                                                    >  1:462419/1‑159                                                                             GCACTTCGGCCATGTGCATCTCGCCAATCACCCGGCGCATCACCTCGATCGGGCAGGTCGCACCGGCCATGATCCCGGTGCGCAGGCTCGACAGGTCGAATTCACCACGCTGTGGATGGTCCAGTTCGGCAATGAACATGGTCGGCACGCCATACAGCG                                                                                                                                                                                                                                                    <  2:462416/159‑1                                                                             GCACTTCGGCCATGTGCATCTCGCCAATCACCCGGCGCATCACCTCGATCGGGCAGGTCGCACCGGCCATGATCCCGGTGCGCAGGCTCGACAGGTCGAATTCACCACGCTGTGGATGGTCCAGTTCGGCAATGAACATGGTCGGCACGCCATACAGC                                                                                                                                                                                                                                                     >  1:1057512/1‑158                                                                                                                                            CGGCCATGATCCCGGTGCGCAGGCTCGACAGGTCGAATTCACCACGCTGTGGATGGTCCAGTTCGGCAATGAACATGGTCGGCACGCCATACAGCGCAGTGGCGCAGGAAAAAGCCACTGCGCTGTATGGCGTGCCGACCATGTTCATTGCCGAACT                                                                                                                                                                                       <  2:433291/157‑1                                                                                                                                                                                                ATGGTCCAGTTCGGCAATGAACATGGTCGGCACGCCATACAGCGCAGTGGCGCAGGAAAAAGCCACTGCGCTGTATGGCGTGCCGACCATGGTCATTGCCGAACTGGACCATCCACAGCGTGGTGAATTCGACCTGTCGAGCCTGCGCACCGGGATCATGGCCGGTGCGACCTGCCCGATC                                                                                                           >  2:1057509/1‑181                                                                                                                                                                                                             GCAATGAACATGGTCGGCACGCCATACAGCGCAGTGGCGCAGGAAAAAGCCACTGCGCTGTATGGCGTGCCGACCATGTTCATTGCCGAACTGGACCATCCACAG                                                                                                                                                                          >  2:908419/1‑105                                                                                                                                                                                                                                           GCAGTGGCGCAGGAAAAAGCCACTGCGCTGTATGGCGTGCCGACCATGTTCATTGCCGAACTGGACCATCCACAGCGTGGTGAATTCGACCTGTCGAGCCTGCGCACCGGGATCATGGCCGG                                                                                                                           >  1:656761/1‑122                                                                                                                                                                                                                                            CAGTGGCGCAGGAAAAAGCCACTGCGCTGTATGGCGTGCCGACCATGTTCATTGCCGAACTGGACCATCCACAGCGTGGTGAATTCGACCTGTCGAGCCTGCGC                                                                                                                                            >  1:28926/1‑104                                                                                                                                                                                                                                            CAGTGGCGCAGGAAAAAGCCACTGCGCTGTATGGCGTGCCGACCATGTTCATTGCCGAACTGGACCATCCACAGCGTGGTGAATTCGACCTGTCGAGCCTGCGC                                                                                                                                            <  2:28926/104‑1                                                                                                                                                                                                                                             AGTGGCGCAGGAAAAAGCCACTGCGCTGTATGGCGTGCCGACCATGTTCATTGCCGAACTGGACCATCCACAGCGTGGTGAATTCGACCTGTCGAGCCTGCGCACCGGGATCATGGCGGGTGCGACCTGCCCGAACGAGGTG                                                                                                     >  2:965333/1‑142                                                                                                                                                                                                                                             AGTGGCGCAGGAAAAAGCCACTGCGCTGTATGGCGTGCCGACCATGTTCATTGCCGAACTGGACCATCCACAGCGTGGTGAATTCGACCTGTCGAGCCTGCGCACCGGGATCATGGCCGGTGCGACCTGCCCGATCGAGGTGATGCGCCGGGTGATTGGCGAGGTGCACAGGGCCG                                                                   >  2:179840/1‑176                                                                                                                                                                                                                                             AGTGGCGCAGGAAAAAGCCACTGCGCTGTATGGCGTGCCGACCATGTTCATTGCCGAACTGGACCATCCACAGCGTGGTGAATTCGACCTGTCGAGCCTGCGCACCGGGATCATGGCCGGTGCGACCTGCCCGATCGAGGTGATGCGCCGGGTGATTGGCGAGATGCACATGGCCGAAGTGCAGATTGCCTATGGCATGACCGAGACCAG                                 >  2:475356/1‑210                                                                                                                                                                                                                                             AGTGGCGCAGGAAAAAGCCACTGCGCTGTATGGCGTGCCGACCATGTTCATTGCCGAACTGGACCATCCACAGCGTGGTGAATTCGACCTGTCGAGCCTGCGCACCGGGATCATGGCCGGTGCGACCTGCCCGATCGAGGTGATGCGCCGGGTGATTGGCGAGATGCACATGGCCGAAGTGCAGATTGCCTATGGCATGACCGAGACCAG                                 <  1:475359/210‑1                                                                                                                                                                                                                                             AGTGGCGCAGGAAAAAGCCACTGCGCTGTATGGCGTGCCGACCATGTTCATTGCCGAACTGGACCATCCACAGCGTGGTGAATTCGACCTGTCGAGCCTGCGCACCGGGATCATGGCCGGGGCGACCTGCCCGATCGAGGTGATGCGCCGGGTGATTGGCGA                                                                                 >  1:377773/1‑162                                                                                                                                                                                                                                                  CGCAGGAAAAAGCCACTGCGCTGTATGGCGTGCCGACCATGTTCATTGCCGAACTGGACCATCCACAGCGTGGTGAATTCGACCTGTCGAGCCTGCGCACCGGGATCATGGCCGGTGCGACCTGCCCGATCGAGGTGATGCGCCGGGTGATTGGCGAGATGCACATGGCCGAAGTGCAGATTGCCTATGGCATGACCGAGACCAGCCCGGTGTCCTTGCAGACCGGTGCCGCTGAC  >  1:758605/1‑236                                                                                                                                                                                                                                                  CGCAGGAAAAAGCCACTGCGCTGTATGGCGTGCCGACCATGTTCATTGCCGAACTGGACCATCCACAGCGTGGTGAATTCGACCTGTCGAGCCTGCGCACCGGGATCATGGCCGGTGCGACCTGCCCGATCGAGGTGATGCGCCGGGTGATTGGCGAGATGCACATGGCCGAAGTGCAGATTGCCTATGGCATGACCGAGACC                                   >  1:277431/1‑203                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               ACGCGACGCTCCAGGTCGTCAGCGGCACCGGTCTGCAAGGACACCGGGCTGGTCTCGGTCATGCCATAGGCAATCTGCACTTCGGCCATGTGCATCTCGCCAATCACCCGGCGCATCACCTCGATCGGGCAGGTCGCACCGGCCATGATCCCGGTGCGCAGGCTCGACAGGTCGAATTCACCACGCTGTGGATGGTCCAGTTCGGCAATGAACATGGTCGGCACGCCATACAGCGCAGTGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/3920651‑3920892 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcgcagtggcGCAGGAAAAAGCCACTGCGCTGTATGGCGTGCCGACCATGTTCATTGCCGAACTGGACCATCCACAGCGTGGTGAATTCGACCTGTCGAGCCTGCGCACCGGGATCATGGCCGGTGCGACCTGCCCGATCGAGGTGATGCGCCGGGTGATTGGCGAGATGCACATGGCCGAAGTGCAGATTGCCTATGGCATGACCGAGACCAGCCCGGTGTCCTTGCAGACCGGTGCCGCTGAC  <  AE015451/3920902‑3920668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.