New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 4833380	59 (0.860)	3 (0.050)	3/320	NT	4.5%	coding (675/801 nt)	PP_4246	Periplasmic binding protein
	?	AE015451	= 4833426	71 (1.090)					coding (629/801 nt)	PP_4246	Periplasmic binding protein
Rejected: Frequency below/above cutoff threshold.

CTGCGCCAGCGTGGCGGTGACTGTGGCTGCGAAGCGGCATCGAACGGCATGGGCTTGACTCACGAAAATGAAGAAAAGGCACACTGCAGGGAACGTTGCCGCAACACGGGAATTTAGTCTTGCGGTGCAGCCACTTTGATTCGGTACGGCTCGAAGATGCGCGCCAGTTCACCGCTGTCACGCAGGCCACGCAGCAGGGCAGCGAACGACTGCTCGCCGATAGGCGCCCCCGGGCGCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/4833141‑4833380 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gggcgcagcTGGTGCCTTCACAACGCATTGACCAGGTCTATCACCACTACGCCTTGCTGCGCCCGGGGGCGCCTATCGGCGAGCAGTCGTTCGCTGCCCTGCTGCGTGGCCTGCGTGACAGCGGTGAACTGGCGCGCATCTTCGAGCCGTACCGAATCAAAGTGGCTGCACCGCAAGACTAAATTCCCGTGTTGCGGCAACGTTCCCTGCAGTGTGCCTTTTCTTCATTTTCGTGAG  <  AE015451/4833426‑4833199                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      CTGCGCCAGCGTGGCGGTGACTGTGGCTGCGAAGCGGCATCGAACGGCATGGGCTTGACTCACGAAAATGAAGAAAAGGCACACTGCAGGGAACGTTGCCGCAACACGGGAATTTAGTCTTGCGGTGCAGCCACTTTGATTCGGTACGGCTCGAAGATGCGCGCCAGTTCACCGCTGTCACGCAGGCCACGCAGCAGGGCAGCGAACGACTGCGCGCCGATAGGCGCCCCCGGGC                                                                                                                                                                                                                                           >  1:654831/1‑235   GCGCCAGCGTGGCGGTGACTGTGGCTGCGAAGCGGCATCGAACGGCATGGGCTTGACTCACGAAAATGAAGAAAAGGCACACTGCAGGGAACGTTGCCGCAACACGGGAATTTAGTCTTGCGGTGCAGCCACTTTGATTCGGTACGGCTCGAAGATGCGCGCCAGTTCACCGCTGTCACGCAGGCCACGCAGCAGGGCAGCGAACGACTGCTCGCCGATAGGCGCCCCCGGGCGCA                                                                                                                                                                                                                                        <  1:566989/236‑1                                                                                                  GCCGCAACACGGGAATTTAGTCTTGCGGTGCAGCCACTTTGATTCGGTACGGCTCGAAGATGCGCGCCAGTTCACCGCTGTCACGCAGGCCACGCAGCAGGGCAGCGAACGACTGCTCGCCGATAGGCGCCCCCGGGCGCAG                                                                                                                                                                                                                                       >  2:40816/1‑142                                                                                                                                                              TGCGCGCCAGTTCACCGCTGTCACGCAGGCCACGCAGCAGGGCAGCGAACGACTGCTCGCCGATAGGCGCCCCCGCGCGCAGCTGGTGCCTTCACAACGCATTGACCAGGTCTATCACCACTACCCCTTGCTGCGCCCGGGGGCGCCTACCGGCGAG                                                                                                                                                            >  2:868570/1‑157                                                                                                                                                                                               GCAGCAGGGCAGCGAACGACTGCTCGCCGATAGGCGCCCCCGGGCGCAGCTGGTGCCTTCACCACGCCTTGACCAGGGCCATCACCACTACGCCTTGCTGCGCCCGGGGGCGCCTATCGGCGCGCAGTCGCTCGCTGCCCT                                                                                                                                           >  2:140036/1‑141                                                                                                                                                                                                 AGCAGGGCAGCGAACGACTGCTCGCCGATAGGCGCCCCCGGGCGCAGCTGGTGCCTTCACAACGCATTGACCAGGTCTATCACCACTACGCCTTGCTGCGCCCGGGGGCGCCTATCGGCGAGCAGTCGTTCGCTGCCCTGCTGCGTGGCCTGCGTGACAGCG                                                                                                                    >  1:943657/1‑162                                                                                                                                                                                                                                         GGCGCAGCTGGTGCCTTCACAACGCATTGACCAGGTCTATCACCACTACGCCTTGCTGCGCCCGGGGGCGCCTATCGGCGAGCAGTCGTTCGCTGCCCTGCTGCGTGGCCTGCGTGACAGCGGTGAACTGGCGCGCATCTTCGAGCCGTACCGAATCAAAGTGGCTGCACCGCAAGACTAAATTCCCGTGTTGCGGCAACGTTCCCTGCAGTGTGCCTTTTCTTCATTTTCGTGAG  <  1:63599/236‑1                                                                                                                                                                                                                                          GCGCAGCTGGTGCCTTCACAACGCATTGACCAGGTCTATCACCACTACGCCTTGCTGCGCCCGGGGGCGCCTATCGGCGAGCAGTCGTTCGCTGCCCTGCTGCGTGGCCTGCGTGACAGCGGTGAACTGGCGCGCATCTTCGAGCCGTACCGAATCAAAGTGGCTGCACCGCAAGACTAAATTCCCGTGTTGCGGCAACGTTCCCTGCAGTGTGCCTTTTCTTCATTTTCGTGA   >  2:673730/1‑234                                                                                                                                                                                                                                               GCTGGTGCCTTCACAACGCATTGACCAGGTCTATCACCACTACGCCTTGCTGCGCCCGGGGGCGCCTATCGGCGAGCAGTCGTTCGCTGCCCTGCTGCGTGGCCTGCGTGACAGCGGTGAACTGGCGCGCATCTTCGAGCCGTACCGAATCAAAGTGGCTGCACCGCAAGACTAAA                                                        <  2:1015985/176‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      CTGCGCCAGCGTGGCGGTGACTGTGGCTGCGAAGCGGCATCGAACGGCATGGGCTTGACTCACGAAAATGAAGAAAAGGCACACTGCAGGGAACGTTGCCGCAACACGGGAATTTAGTCTTGCGGTGCAGCCACTTTGATTCGGTACGGCTCGAAGATGCGCGCCAGTTCACCGCTGTCACGCAGGCCACGCAGCAGGGCAGCGAACGACTGCTCGCCGATAGGCGCCCCCGGGCGCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/4833141‑4833380 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gggcgcagcTGGTGCCTTCACAACGCATTGACCAGGTCTATCACCACTACGCCTTGCTGCGCCCGGGGGCGCCTATCGGCGAGCAGTCGTTCGCTGCCCTGCTGCGTGGCCTGCGTGACAGCGGTGAACTGGCGCGCATCTTCGAGCCGTACCGAATCAAAGTGGCTGCACCGCAAGACTAAATTCCCGTGTTGCGGCAACGTTCCCTGCAGTGTGCCTTTTCTTCATTTTCGTGAG  <  AE015451/4833426‑4833199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.