| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 4,022,307 | G→C | 8.8% | intergenic (‑2/+666) | PP_3547 ← / ← emrB | putative NAD(P)‑binding oxidoreductase with NAD(P)‑binding Rossmann‑fold domain/multidrug efflux transport system ‑ permease subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 4,022,307 | 0 | G | C | 8.8% | 225.1 / 13.5 | 68 | intergenic (‑2/+666) | PP_3547/emrB | putative NAD(P)‑binding oxidoreductase with NAD(P)‑binding Rossmann‑fold domain/multidrug efflux transport system ‑ permease subunit |
| Reads supporting (aligned to +/- strand): ref base G (30/32); new base C (4/2); total (34/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01 | |||||||||||
GCCGGTGATGACAATGACGTTCTCCATGGGTTTGCCAATGGTTTGGGGAAGGGAACATCGACAATAGGCGAAATCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGAAAAGGTGAGTCAGCGTCGCCATCGCAAACGGACGGTTCGCTGAGTCAA > AE015451/4022084‑4022527 | gccgGTGATGACAATGACGTTCTCCATGGGTTTGCCAATGGTTTGGGGAAGGGAACATCGACAATAGGCGAAATCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCaa > 1:731607/1‑235 (MQ=255) ggTGATGACAATGACGTTCTCCATGGGTTTGCCAATGGTTTGGGGAAGGGAACATCGACAATAGGCGAAATCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGT < 1:1237211‑M1/229‑1 (MQ=255) tgatgaCAATGACGTTCTCCATGGGTTTGCCAATGGTTTGGGGAAGGGAACATCGACAATAGGCGAAATCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAgcgc < 2:979549/234‑1 (MQ=255) gACGTTCTCCATGGGTTTGCCAATGGTTTGGGGAAGGGAACATCGACAATAGGCGAAATCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGA > 1:934612‑M1/1‑210 (MQ=255) ccATGGGTTTGCCAATGGTTTGGGGAAGGGAACATCGACAATAGGCGAAATCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGACCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGGCCGTCAAGCGATGAGGCCCCa > 2:685033/1‑226 (MQ=255) nggTTTGCCAATGGTTTGGGGAAGGGAACATCGACAATAGGCGAAATCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCg < 2:517225/235‑1 (MQ=255) gggTTTGCCAATGGTTTGGGGAAGGGAACATCGACAATAGGCGAAATCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCg < 1:965515/236‑1 (MQ=255) gTTTGCCAATGGTTTGGGGAAGGGAACATCGACAATAGGCGAAATCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGc < 2:522536/229‑1 (MQ=255) gTTTGCCAATGGTTTGGGGAAGGGAACATCGACAATAGGCGAAATCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGc > 1:522539/1‑229 (MQ=255) gTTTGCCAATGGTTTGGGGAAGGGAACATCGACAATAGGCGAAATCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCg < 2:1078172/234‑1 (MQ=255) ttGCCAATGGTTTGGGGAAGGGAACATCGACAATAGGCGAAATCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTGCAgcgc > 1:834560/1‑236 (MQ=255) cAATGGTTTGGGGAAGGGAACATCGACAATAGGCGAAATCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAgc < 2:548474/202‑1 (MQ=255) cAATGGTTTGGGGAAGGGAACATCGACAATAGGCGAAATCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAgc > 1:548477/1‑202 (MQ=255) cAATGGTTTGGGGAAGGGAACATCGACAATAGGCGAAATCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTc > 1:457668/1‑236 (MQ=255) cAATGGTTTGGGGAAGGGAACATCGACAATAGGCGAAATCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGAT > 2:1194674‑M1/1‑191 (MQ=255) ggggAAGGGAACATCGACAATAGGCGAAATCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGc < 1:374292/214‑1 (MQ=255) ggggAAGGGAACATCGACAATAGGCGAAATCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCgg < 1:658708/234‑1 (MQ=255) aaCATCGACAATAGGCGAAATCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCg > 1:634388/1‑236 (MQ=255) aaCATCGACAATAGGCGAAATCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGC > 2:956321‑M1/1‑175 (MQ=255) cATCGACAATAGGCGAAATCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGc < 1:92425/232‑1 (MQ=255) aTAGGCGAAATCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGcgcg > 1:590760/1‑205 (MQ=255) aTAGGCGAAATCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGcgcg < 2:590757/205‑1 (MQ=255) ggCGAAATCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCCTGGATGCCCGTCAcctcccttcgcaagccctgcgctcggcctcctgaagccgcaatccgcg > 2:787201‑M1/1‑168 (MQ=255) aaaTCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCggg > 2:826717/1‑209 (MQ=255) aaTCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATCCTGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGa < 1:566227/236‑1 (MQ=255) aTCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTg < 1:479082/169‑1 (MQ=255) cgcgCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGcgcg > 1:791534/1‑194 (MQ=255) cgcgCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGcgcg < 2:791531/194‑1 (MQ=255) cTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGagcgca < 2:1057358/234‑1 (MQ=255) tGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCGCGGagcgcagc > 1:786173/1‑235 (MQ=255) gCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGagcgcagcg < 1:789570/235‑1 (MQ=255) ttGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAgg < 1:62250/236‑1 (MQ=255) tGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGa > 2:632744/1‑207 (MQ=255) tGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAgcgcagcgca > 2:1201040/1‑233 (MQ=255) aaCATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGagcgcagcgc > 2:578285/1‑222 (MQ=255) aCATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAcctcccttcgcaagccctgcgctcggcctcctgaagtcgcaatccgcggcgcctgggctatcgcgcgcttagaagcaaaatcaaaagccgaagcgag < 1:956324‑M1/236‑98 (MQ=255) cAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCg < 1:968763/164‑1 (MQ=255) cAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCg > 2:968760/1‑164 (MQ=255) cAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAgg < 2:871891/220‑1 (MQ=255) cAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGt > 2:557943/1‑235 (MQ=255) ctctGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATCCATGCCCGTCAcctcccttcgcaagccctgcgctcggcctcctgaagtcgcaatccgcggcgcctgggctatcgcgcgcttagaagcaaaatcaaaagccgaagcgaga > 1:858014‑M1/1‑121 (MQ=255) ctGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAcctcccttcgcaagccctgcgctcggcctcctgaagtcgcaatccgcggcgcctgggctatcgcgcgcttagaagcaaaatcaaaagccgaagcgagagcgagagcgagagcgaga < 1:733656‑M1/235‑117 (MQ=255) ggttttgatctggcttttgatctggcTTTTGATCTTGATCTTGGTCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGa < 1:271503/161‑1 (MQ=255) tttgatctggcttttgatctggctTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAcctcccttcgcaagccctgcgctcggcctcctgaagtcgcaatccgcggcgcctgggctatcgcgcgcttagaagcaaaatcaaaagccgaagcgagagcgagagcgagagcgagagcga > 2:1005485‑M1/1‑114 (MQ=255) ctggcttttgatctggcttttgatCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGAT > 2:1139094‑M1/1‑100 (MQ=255) ctggcttttgatctggcttttgatCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGAT < 1:1139097‑M1/100‑1 (MQ=255) ttttgatctggcttttgatctTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAACGCAGGGGCCGGATGATGGGAGCGCAGCg > 1:521546/1‑211 (MQ=39) ggcttttgatctTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACTTTTTGTCGCGTTTg < 2:834557/228‑1 (MQ=11) cttttgatctTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATCCATGCCCGTCAAGCGCTG‑GGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGC‑CAGCGTTTTTTGGTTACTTTTTGTCGCGGTTGACaaa > 1:859570/1‑230 (MQ=11) tgatcttgatcttgatcttCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCg < 1:588996/235‑1 (MQ=31) cttgatcttgatcttCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAgg > 1:1082078/1‑236 (MQ=31) tgatcttgatcttCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGGGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAg < 1:604526/182‑1 (MQ=14) tgatcttgatcttCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATCCATGCCCGTCCcctcccttcgcaagccctgcgctcggcctcctgaagtcgcaatccgcggcgcctgggctatcgcgcgcttagaagcaaaatcaaaagccgaagcgagagcgagagcgagagcgagagc < 1:1006520‑M1/194‑119 (MQ=255) tgatcttCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGaaa < 2:634385/234‑1 (MQ=33) gACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGAAAAGGTGAGTCAGc > 1:1163335/1‑236 (MQ=33) aggaggCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAcctcccttcgcaagccctgcgctcggcctcctgaagtcgcaatccgcggcgcctgggctatcgcgcgcttagaagcaaaatcaaaagccgaagcgagagcgagagcgagagcgagagcgagagcgagagcatcgggatcttgccttgatttta < 1:1005488‑M1/207‑154 (MQ=255) gaggCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAcctcccttcgcaagccctgcgctcggcctcctgaagtcgcaatccgcggcgcctgggctatcgcgcgcttagaagcaaaatcaaaagccgaagcgagagcgagagcgagagcgagagcgagagcgagagcatcgggatcttgccttgattttagctcaatgccccgcc > 2:908129‑M1/1‑52 (MQ=255) aCGCAGGCCTTGCGGAGGCAGGTGACGGGCATCCATGCCCGTCAcctcccttcgcaagccctgcgctcggcctcctgaagtcgcaatccgcggcgcctgggctatcgcgcgcttagaagcaaaatcaaaagccgaagcgagagcgagagcgagagcgagagcgagagcgagagcatcgggatcttgc < 1:1061127‑M1/187‑144 (MQ=255) gCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGAAAAGGTGAGTCAGCGTCGCCATCGCAAacgg > 1:1204358/1‑235 (MQ=38) gCAGGCCTTGCGGAGGCAGGTGACGGGCATCCATGCCCGTCAcctcccttcgcaagccctgcgctcggcctcctgaagtcgcaatccgcggcgcctgggctatcgcgcgcttagaagcaaaatcaaaagccgaagcgagagcgagagcgagagcgagagcgagagcgagagcatcgggatcttgccttgattttagctcaatgccccgccgatgccgggcccgccttagcggta > 2:1129470‑M1/1‑42 (MQ=255) cAGGCCTTGCGGAGGCAGGGGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGAAAAGGTGAGTCAGCGTCGCCATCGCAAacg < 2:878681/233‑1 (MQ=37) ccTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTCTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGAAAAGGTGAGTCAGCGTCGCCATCGCAAACGGACGGtt < 1:176480/236‑1 (MQ=38) cGGAGGCAGGTGACGGGCATCCATGCCCGTCAcctcccttcgcaagccctgcgctcggcctcctgaagtcgcaatccgcggcgcctgggctatcgcgcgcttagaagcaaaatcaaaagccgaagcgagagcgagagcgagagcgagagcgagagcgagagcatcgggatcttgccttgattttagctcaatgccccgccgatgccgggcccgccttagcggtaaagggcggcttg > 1:56832‑M1/1‑32 (MQ=255) ggagggagGTGACGGGTATGGATGCCCGTCAAGAGCTGAGGCCCCAGGTTGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAAcc < 2:1131021/99‑1 (MQ=11) ggAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAACGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGc < 2:521543/182‑1 (MQ=14) gCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCGTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCTGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCg > 1:442367/1‑177 (MQ=14) gcttgacGGGCATGGATGCCCGTCAcctcccttcgcaagccctgcgctcggcctcctgaagtcgcaatccgcggcgcctgggctatcgcgcgcttagaagcaaaatcaaaagccgaagcgagagcgagagcgagagcgagagcgagagcgagagcatcgggatcttgccttgattttagctcaatgccccgccgatgccgggcccgccttagcggcaaagggcggcttggcgaac > 1:845376‑M1/4‑25 (MQ=255) aTGGATGCCCGTCAAGCGCTGAGGCCCCAGGAGGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGt > 2:178609/1‑123 (MQ=17) | GCCGGTGATGACAATGACGTTCTCCATGGGTTTGCCAATGGTTTGGGGAAGGGAACATCGACAATAGGCGAAATCGCGCTGCGTTGGAATGACGAACATCAGCACAGCCAACCCTCTGCTTTTGATCTGGCTTTTGATCTGGCTTTTGATCTTGATCTTGATCTTGATCTTCGCGACTTCAGGAGGCCGAACGCAGGCCTTGCGGAGGCAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGATGGGGCCTTCGGCGCGGTCCTGCCGGGAGCAAGGCCGGAGTGAGGGAACCCCGGAGCGCAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACTCGCCGTAAGGGCGAAAAGGTGAGTCAGCGTCGCCATCGCAAACGGACGGTTCGCTGAGTCAA > AE015451/4022084‑4022527 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |