Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	AE015451	4,741,276	G→C	100%	intergenic (+513/+240)	gltA → / ← yeiW	citrate synthase/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	4,741,276	0	G	C	100.0%	286.0 / NA	75	intergenic (+513/+240)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (36/39);  total (36/39)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTTCCGGCAGGTAAGTCGGCTCTTCTTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGA‑TGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AACCTCC‑GCCAAC‑TGCAATTCGTGAA‑CCAAGCAAAAGGCCCCGAAAAGGCCCTC‑C‑GCAGCCCCCCCAACCTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGAACACCCACACCACACAGCAGTGCAATACGCTTGAACGATCTCATCATGTTTATCCTTGTTGT  >  AE015451/4741072‑4741500                                                                                                                                                                                                                  |                                                                                                                                                                                                                                   ctTCCGGCAGGTAAGTCGGCTCTTCTTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGA‑TGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAg                                                                                                                                                                                                     >  2:1011584/1‑235 (MQ=255)  ttCCGGCAGCTCAGTCGGCTCTTCTTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGA‑TGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCa                                                                                                                                                                                                      <  1:834158/233‑1 (MQ=255)          ggTAAGTCGGCTCTTCTTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGA‑TGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGgcgc                                                                                                                                                                                               >  2:673713/1‑232 (MQ=255)              attCGGCTCTTCTTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGA‑TGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATc                                                                                                                                                                                        <  1:491475/233‑1 (MQ=255)                tCGGCTCTTCTTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGA‑TGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCAc                                                                                                                                                                                      <  1:182985/235‑1 (MQ=255)                  ggCTCGTCTTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGA‑TGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGtcctc‑ct                                                                                                                                                                                                                           <  1:65049/196‑2 (MQ=255)                   gCTCTTCTTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGA‑TGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTc                                                                                                                                                                                  <  1:673716/236‑1 (MQ=255)                     tcttctTCCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGA‑TGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTca                                                                                                                                                                                 <  1:254810/235‑1 (MQ=255)                             cAGTTTACGGGTCAGGCTGCCATCCGCCTCCTTGA‑TGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGa                                                                                                                                                                         >  2:633773/1‑235 (MQ=255)                             cAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGA‑TGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATCGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTca                                                                                                                                                                                 <  2:536016/227‑1 (MQ=255)                                 ttACGGGTCAGGCTGCCATCCGCCTCCTTGATTGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAgg                                                                                                                                                                     >  1:420541/1‑236 (MQ=255)                                             cTGCCATCCGCCTCCTTGA‑TGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCT‑GCGACT‑TGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTg                                                                                                                                                          >  2:991576/1‑234 (MQ=255)                                                 cctCCGCCTCCTTGA‑TGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACT‑TGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc                                                                                                                                                                    <  2:941281/218‑1 (MQ=255)                                                          cctTGA‑TGGTCGTAATGTGCATCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTTGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGgcgc                                                                                                                                                                                               <  1:457545/184‑1 (MQ=255)                                                              gA‑TGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGa                                                                                                                                       >  1:895503/1‑236 (MQ=255)                                                               a‑TGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAg                                                                                                                                      >  1:65812/1‑236 (MQ=255)                                                               a‑TGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCATGCTCGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAg                                                                                                                                      <  1:652571/236‑1 (MQ=255)                                                                    tCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGATCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCAT‑GCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTg                                                                                                                                        <  2:650861/230‑1 (MQ=255)                                                                         aTGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGAccc                                                                                                                                                     >  1:992474/1‑212 (MQ=255)                                                                         aTGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGAccc                                                                                                                                                     <  2:992471/212‑1 (MQ=255)                                                                                gcaCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCT‑GCGACT‑TGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTg                                                                                                                                                          <  2:929013/200‑1 (MQ=255)                                                                                 caCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCa                                                                                                                                                                                       >  2:69224/1‑170 (MQ=255)                                                                                  aCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgt                                                                                                                     <  1:1011587/235‑1 (MQ=255)                                                                                    ggAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCT‑GCGACT‑TGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtc                                                                                                                    >  2:1106805/1‑234 (MQ=255)                                                                                    ggAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCTAGCGGCGCCTTGATCACGGTCACACcgc                                                                                                                                                                           >  1:356505/1‑179 (MQ=255)                                                                                     gAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCT‑GCGACT‑TGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcct                                                                                                                  >  1:712245/1‑235 (MQ=255)                                                                                         cACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcctcgtcc                                                                                                             >  1:425249/1‑236 (MQ=255)                                                                                           cgcgGCGTAACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                                                                                                                           <  2:594219/172‑1 (MQ=255)                                                                                             cggcgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCgg                                                                                                                                                   <  2:1205137/194‑1 (MQ=255)                                                                                              ggcgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCCCCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCg                                                                                                                                                    <  2:186512/192‑1 (MQ=255)                                                                                              ggcgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGc                                                                                                        >  1:614084/1‑236 (MQ=255)                                                                                              ggcgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCT‑GCGACT‑TGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGc                                                                                                        >  1:473379/1‑236 (MQ=255)                                                                                              ggcgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGAc                                                                                                                                                       >  1:6783/1‑189 (MQ=255)                                                                                              ggcgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGa                                                                                                                                   >  1:483893/1‑209 (MQ=255)                                                                                               gcgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCa                                                                                                                                                <  2:483890/195‑1 (MQ=255)                                                                                                   cACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCT‑GCGACT‑TGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACt                                                                                                    >  1:484650/1‑235 (MQ=255)                                                                                                   cACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTg                                                                                                                                        <  1:634214/199‑1 (MQ=255)                                                                                                    aCCGGACATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCAc                                                                                                                                          <  2:1073924/196‑1 (MQ=255)                                                                                                     ccGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCtt                                                                                                                                                                                            <  2:141701/145‑1 (MQ=255)                                                                                                       ggCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCt                                                                                                                                             <  1:25080/190‑1 (MQ=255)                                                                                                            tGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTTCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCa                                                                                                                                                <  2:519745/182‑1 (MQ=255)                                                                                                             ggCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGttt                                                                                           >  2:548128/1‑234 (MQ=255)                                                                                                                gTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCT‑GCGACT‑TGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCCCTTGCCAGt                                                                                             >  2:936479/1‑229 (MQ=255)                                                                                                                gTATTGAACT‑GGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGcc                                                                                     >  1:823086/1‑236 (MQ=255)                                                                                                                   ttGACCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGCCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAgcggcg                                                                                                                                                                                                <  2:81494/127‑1 (MQ=255)                                                                                                                      aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACCGTCACACCGCGAt                                                                                                                                                                        >  2:108258/1‑148 (MQ=255)                                                                                                                             tataGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCcaca                                                                            <  2:425246/233‑1 (MQ=255)                                                                                                                              ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAg                                                                           >  2:930868/1‑233 (MQ=255)                                                                                                                                     cAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGAc                                                                      <  1:1220645/231‑1 (MQ=255)                                                                                                                                     cAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGAc                                                                      >  2:1220642/1‑231 (MQ=255)                                                                                                                                      aGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgt                                                                                                                     <  1:1136058/183‑1 (MQ=255)                                                                                                                                        ctctGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGAcag                                                                    >  2:451747/1‑230 (MQ=255)                                                                                                                                        ctctGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGcc                                                                                                       >  2:764612/1‑195 (MQ=255)                                                                                                                                        ctctGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCa                                                                                   >  1:16568/1‑215 (MQ=255)                                                                                                                                        ctctGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGaa                                                               >  1:70371/1‑235 (MQ=255)                                                                                                                                        ctctGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTAGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGAc                                                                      >  1:978627/1‑228 (MQ=255)                                                                                                                                        ctctGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATCCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCtag                                                                                                                                                                                           >  2:357670/1‑109 (MQ=255)                                                                                                                                         tctGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACCCCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAgg                                                                          >  2:777113/1‑223 (MQ=255)                                                                                                                                         tctGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TGTCACTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGAcag                                                                    <  1:369432/229‑1 (MQ=255)                                                                                                                                          ctGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGAcagcag                                                                 >  1:1147505/1‑231 (MQ=255)                                                                                                                                          ctGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGAcagc                                                                   >  1:284103/1‑229 (MQ=255)                                                                                                                                          ctGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGAacac                                                            >  1:1190014/1‑236 (MQ=255)                                                                                                                                           tGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGCA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGAacac                                                            <  1:137528/235‑1 (MQ=255)                                                                                                                                           tGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGAacac                                                            <  1:737887/235‑1 (MQ=255)                                                                                                                                           tGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGAacac                                                            <  1:474295/235‑1 (MQ=255)                                                                                                                                            ggTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGAcag                                                                    <  2:887522/226‑1 (MQ=255)                                                                                                                                                aCCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGGCACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGAcagc                                                                   >  1:332747/1‑223 (MQ=255)                                                                                                                                                aCCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAgg                                                                          <  2:528270/216‑1 (MQ=255)                                                                                                                                                aCCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGAGATAGGCCTTGTACTTGGAc                                                                                                                                                       >  2:1270573/1‑139 (MQ=255)                                                                                                                                                    tCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGAACACccacacc                                                     <  2:823083/233‑1 (MQ=255)                                                                                                                                                                         tgcgacttgcaTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGAACACCCACACCACACAGCAGTGCAATACGCtt                                <  1:996637/226‑1 (MQ=255)                                                                                                                                                                                 gcaTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGt                                                                                             <  2:468868/165‑1 (MQ=255)                                                                                                                                                                                   aTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGAACACCCACACCACACAGCAGt                                           <  1:669276/215‑1 (MQ=255)                                                                                                                                                                                              ccaggcacaGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTAGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGAACACccacaccaca                                                  <  1:983626/190‑1 (MQ=255)                                                                                                                                                                                                        gCCCCGGACACGGCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGAACACCCACACCACACAGCAGTGCAATACGCTTGAACGATCTCATCATGTTTATCCTtgttgt  <  1:1052776/236‑1 (MQ=255)                                                                                                                                                                                                                  |                                                                                                                                                                                                                                   CTTCCGGCAGGTAAGTCGGCTCTTCTTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGA‑TGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AACCTCC‑GCCAAC‑TGCAATTCGTGAA‑CCAAGCAAAAGGCCCCGAAAAGGCCCTC‑C‑GCAGCCCCCCCAACCTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGAACACCCACACCACACAGCAGTGCAATACGCTTGAACGATCTCATCATGTTTATCCTTGTTGT  >  AE015451/4741072‑4741500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑