| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 4,864,410 | T→A | 6.0% | Q488L (CAG→CTG) ‡ | smc ← | Chromosome partition protein Smc |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 4,864,410 | 0 | T | A | 6.0% | 241.7 / 5.6 | 67 | Q488L (CAG→CTG) ‡ | smc | Chromosome partition protein Smc |
| Reads supporting (aligned to +/- strand): ref base T (28/35); new base A (2/2); total (30/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-02 | |||||||||||
TCCAGGCCGGCAAAATCAAGGTCGTTGAAATCGTCCACCAGCACAGCCTGCAGATCGGCGCCAAGCACCGTCTCTACCGCCAGCTCCCAACCCGGCTCCACCCGCAAGCCTTCGGCCAGCCGCGGCTGTTGCTCCAGCCCTTGGCCGTGCAGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCGCAGAGCTGCAGTTCTTCCAGCAGCATTTCGCTGCTGGCCAGTTGCTCGGCCAGTTCGAGCATGGCGGCATCCTGCGGGTCGCTGCCCAGTTGCTCGCGCTCCTCGACCAACTTGCGCTGGCGCTCGGCCTGGCG > AE015451/4864179‑4864633 | tCCAGGCCGGCAAAATCAAGGTCGTTGAAATCGTCCACCAGCACAGCCTGCAGATCGGCGCCAAGCACCGTCTCTACCGCCAGCTCCCAACCCGGCTCCACCCGCAAGCCTTCGGCCAGCCGCGGCTGTTGCTCCAGCCCTTGGCCGTGCAGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCa < 1:961702/235‑1 (MQ=255) aGGCCGGCAAAATCAAGGTCGTTGAAATCGTCCACCAGCACAGCCTGCAGATCGGCGCCAAGCACCGTCTCTACCGCCAGCTCCCAACCCGGCTCCACCCGCAAGCCTTCGGCCAGCCGCGGCTGTTGCTCCAGCCCTTGGCCGTGCAGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCCGGt > 1:1080653/1‑235 (MQ=255) gCCGGCAAAATCCAGGTCGTTGAAATCGTCCACCAGCACAGCCTGCAGATCGGCGCCAAGCACCGTCTCTACCGCCAGCTCCCAACCCGGCTCCACCCGCAAGCCTTCGGCCAGCCGCGGCTGTTGCTCCAGCCCTTGGCCGTGCAGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCg < 1:1015754/235‑1 (MQ=255) aaaTCAAGGTCGTTGAAATCGTCCACCAGCACAGCCTGCAGATCGGCGCCAAGCACCGTCTCTACCGCCAGCTCCCAACCCGGCTCCACCCGCAAGCCTTCGGCCAGCCGCGGCTGTTGCTCCAGCCCTTGGCCGTGCAGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGc > 1:599414/1‑236 (MQ=255) cAAGGTCGTTGAAATCGTCCACCAGCACAGCCTGCAGATCGGCGCCAAGCACCGTCTCTACCGCCAGCTCCCAACCCGGCTCCACCCGCAAGCCTTCGGCCAGCCGCGGCTGTTGCTCCAGCCCTTGGCCGTGCAGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCctgc < 2:599411/236‑1 (MQ=255) aGCCTGCAGATCGGCGCCAAGCACCGTCTCTACCGCCAGCTCCCAACCCGGCTCCACCCGCAAGCCTTCGGCCAGCCGCGGCTGTTGCTCCAGCCCTTGGCCGTGCAGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCctgctg < 1:22047/210‑1 (MQ=255) gCCTGCAGATCGGCGCCAAGCACCGTCTCTACCGCCAGCTCCCAACCCGGCTCCACCCGCAAGCCTTCGGCCAGCCGCGGCTGTTGCTCCAGCCCTTGGCCGTGCAGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCctg < 1:679738/206‑1 (MQ=255) gCAGATCGGCGCCAAGCACCGTCTCTACCGCCAGCTCCCAACCCGGCTCCACCCGCAAGCCTTCGGCCAGCCGCGGCTGTTGCTCCAGCCCTTGGCCGTGCAGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTg > 2:420320/1‑208 (MQ=255) aTCGGCGCCAAGCACCGTCTCTACCGCCAGCTCCCAACCCGGCTCCACCCGCAAGCCTTCGGCCAGCCGCGGCTGTTGCTCCAGCCCTTGGCCGTGCAGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGt < 2:911386/213‑1 (MQ=255) tCGGCGCCAAGCACCGTCTCTACCGCCAGCTCCCAACCCGGCTCCACCCGCAAGCCTTCGGCCAGCCGCGGCTGTTGCTCCAGCCCTTGGCCGTGCAGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTcgcgcgc > 1:296654/1‑236 (MQ=255) ccccGTCTCTACCGCCAGCTCCCAACCCGGCTCCACCCGCAAGCCTTCGGCCAGCCGCGGCTGTTGCTCCAGCCCTTGGCCGTGCAGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTc < 1:229024/172‑1 (MQ=255) aCCGTCTCTACCGCCAGCTCCCAACCCGGCTCCACCCGCAAGCCTTCGGCCAGCCGCGGCTGTTGCTCCAGCCCTTGGCCGTGCAGCCATTGCGCAGCCCCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGCCGCCCACCCACGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGc < 1:664579/203‑1 (MQ=255) cGCCAGCTCCCAACCCGGCTCCACCCGCAAGCCTTCGGCCAGCCGCGGCTGTTGCTCCAGCCCTTGGCCGTGCAGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCt < 1:579367/236‑1 (MQ=255) cGCCAGCTCCCAACCCGGCTCCACCCGCAAGCCTTCGGCCAGCCGCGGCTGTTGCTCCAGCCCTTGGCCGTGCAGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCt < 1:469913/236‑1 (MQ=255) gCCAGCTCCCAACCCGGCTCCACCCGCAAGCCTTCGGCCAGCCGCGGCTGTTGCTCCAGCCCTTGGCCGTGCAGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATccc > 2:469910/1‑231 (MQ=255) tCCCACCCCGGCTCCACCCGCAAGCCTTCGGCCAGCCGCGGCTGTTGCTCCAGCCCTTGGCCGTGCAGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTgggcgcctgg < 1:1273358/192‑9 (MQ=255) cccAACCCGGCTCCACCCGCAAGCCTTCGGCCAGCCGCGGCTGTTGCTCCAGCCCTTGGCCGTGCAGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCg > 1:146038/1‑236 (MQ=255) cccAACCCGGCTCCACCCGCAAGCCTTCGGCCAGCCGCGGCTGTTGCTCCAGCCCTTGGCCGTGCAGCCATTGCGCAGCACCTGCGCACGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCg > 1:662117/1‑236 (MQ=255) ccGGCTCCACCCGCAAGCCTTCGGCCAGCCGCGGCTGTTGCTCCAGCCCTTGGCCGTGCAGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCg > 2:673883/1‑230 (MQ=255) ccACCCGCAAGCCTTCGGCCAGCCGCGGCTGTTGCTCCAGCCCTTGGCCGTGCAGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCctgctg > 2:1166055/1‑178 (MQ=255) ccACCCGCAAGCCTTCGGCCAGCCGCGGCTGTTGCTCCAGCCCTTGGCCGTGCAGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTc < 2:971206/223‑1 (MQ=255) ccACCCGCAAGCCTTCGGCCAGCCGCGGCTGTTGCTCCAGCCCTTGGCCGTGCAGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTc > 1:971209/1‑223 (MQ=255) ccACCCGCAAGCCTTCGGCCAGCCGCGGCTGTTGCTCCAGCCCTTGGCCGTGCAGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCGCAGAGCTGCAGt > 1:206726/1‑236 (MQ=255) cACCCGCAGGCCTGCGGCCAGCCGCGGCTGGTGCTCCAGCCCTTGGCCGTGCAGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCctgc < 2:301124/154‑1 (MQ=255) cACCCGCAAGCCTTCGGCCAGCCGCGGCTGTTGCTCCAGCCCTTGGCCGTGCAGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCGCAGAGcctgtctc > 1:66144/1‑229 (MQ=255) aaGCCTTCGGCCAGCCGCGGCTGTTGCTCCAGCCCTTGGCCGTGCAGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCGCAGAGCTGCAGTTCTTCcag < 1:1224930/236‑1 (MQ=255) tCGGCCAGCCGCGGCTGTTGCTCCAGCCCTTGGCCGTGCAGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCGCAGAGCTGCAGTTCTTCCAGCAGCAt > 1:825566/1‑236 (MQ=255) ggCCAGCCGCGGCTGTTGCTCCAGCCCTTGGCCGTGCAGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCAACAGCAGGCACAGGGCGACCTGCAGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCGCAGAGCTGCAGTTCTTCCAGCAGCAtt < 1:762023/235‑1 (MQ=255) gCCAGCCGCGGCTGTTGCTCCAGCCCTTGGCCGTGCAGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCAACAGCAGGCACAGGGCGACCTGCAGCGCCTGGGTGGCCTGCTGCAGTTGCTcgc < 1:1080227/172‑1 (MQ=25) gCCAGCCGCGGCTGTTGCTCCAGCCCTTGGCCGTGCAGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCAACAGCAGGCACAGGGCGACCTGCAGCGCCTGGGTGGCCTGCTGCAGTTGCTcgc > 2:1080224/1‑172 (MQ=25) cgGCTGTTGCTCCAGCCCTTGGCCGTGCAGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCGCAGAGCTGCAGTTCTTCCAGCAGCATTTCGCTGCTgg < 1:627174/236‑1 (MQ=255) cTGTTGCGCCAGCCCTTGGCCGTGCAGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCctgc < 2:736704/127‑1 (MQ=255) tCCAGCCCTTGGCCGTGCAGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCGCAGAGCTGCAGTTCTTCCAGCAGCATTTCGCTGCTGGCCAGTTGCTc < 1:342590/236‑1 (MQ=255) gCCGTGCAGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGgc < 1:179474/158‑1 (MQ=255) ccGTGCAGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGCGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTccg > 2:299958/1‑153 (MQ=255) ccGTGCAGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCGCAGAGCTGCAGTTCTTCCAGCAGCATTTCGCTGCTGGCCAGTTGCTCGGCCAGTTCGa < 1:968881/235‑1 (MQ=255) aGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCGCAGTGCTGCa > 2:932884/1‑181 (MQ=255) aGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCGCAGAGCTGCAGTTCTTccc > 1:1000480/1‑189 (MQ=255) aGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCGCAGAGCTGCAGTTCTTCCAGCAGCATTTCGCTGCTGGCCAGTTGCTCGGCCAGTTCg > 2:885242/1‑228 (MQ=255) aGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCGCAGAGCTGCAGTTCTTCCAGCAGCATTTCGCTGCTGGCCAGTTGCTCGGCCAGTTCGAGCATgg > 1:770933/1‑235 (MQ=255) aGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCGCAGAGCTGCAGTTCTTCCAGCAGCATTTCGCTGCTGGCCAGTTGCTCGGCCAGTTCGAGCATgg > 1:969145/1‑235 (MQ=255) aGCCATTGCGCAGCACCTGCGCCCGACTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCAACAGCAGGCACAGGGCGACCTGCAGCGCCTGGGTGGCCTGCTGCAGtcg > 2:63933/1‑129 (MQ=18) ccATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGctct < 1:60364/166‑1 (MQ=255) aTTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCctg < 2:76074/97‑1 (MQ=255) cgcAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGCGGCCAGGGGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGCCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCGCAGAGCTGCAGTTCTTCCAGCAGCATTTCGCTGCTGGCCAGTTGCTCGGCCAGTTCGAGCATGGCGGCATCCt < 1:762087/236‑1 (MQ=255) cgcAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCGCAGAGCTGCAGTTCTTCCAGCAGCATTTCGCTGCTGGCCAGTTGCTCGGCCAGTTCGAGCa < 2:825563/224‑1 (MQ=255) cgcAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCGCAGAGCTGCAGTTCTTCCAGCAGCATTTCGCTGCTGGCCAGTTGCTCGGCCAGTTCGAGCATGGCGGCATcc < 1:1078926/235‑1 (MQ=255) caCCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCGCAGAGCTGCAGTTCTTCCAGCAGCATTTCGCTGCTGGCCAGTTGCTCGGCCAGTTCGAGCATGGCGGCATCCTGCgg > 1:1089605/1‑235 (MQ=255) cTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCGCAGAGCTGCAGTTCTTCCAGCAGCATTTCGCTGCTGGCCAGTTGCTc < 2:296653/202‑1 (MQ=255) tGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCGCAGAGCTGCAGTTCTTCCAGCAGCATTTCGCTGCTGGCCAGTTGCTCGGCCAGTTCGAGCATGGCGGCATCCTGCGGGTCg > 1:669682/1‑235 (MQ=255) tGCGCCCGGCGCCAGGGCGGCCTGCTGCACGGCTTGCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCt < 2:272925/95‑1 (MQ=255) cgcCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCctg < 2:279769/82‑1 (MQ=255) ccGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCt < 2:66144/141‑1 (MQ=255) ctgctgCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCGCAGAGCTGCAGTTCTTCCAGCAGCATTTCGCTGCTGGCCAGTTGCTCGGCCAGTTCGAGCATGGCGGCATCCTGCGGGTCGCTGCCCAGTTGCTCGCGctcct < 1:480018/236‑1 (MQ=255) tgctgcAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCGCAGAGCTGCAGTTCTTCCAGCAGCATTTCGCTGCTGGTCAGTTGCTCTGCCAGTTCGAg > 1:241097/1‑191 (MQ=255) gctgcAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCGCAGAGCTGCAGTTCTTCCAGCAGCATTTCGCTGCTGGCCAGTTGCTCGGCCAGTTCGAg > 1:936030/1‑190 (MQ=255) accAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCGCAGAGCTGCAGTTCTTCCAGCAGCATTTCGCTGCTGGCCAGTTGCTCGGCCAGTTCGAGCATGGCGGCATCCTGCGGGTCGCTGCCCAGTTGCTCGCGCTCCTCGACCAACTTgc < 1:694797/234‑1 (MQ=255) gCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCGCAGAGCTGCAGTTCTTCCAGCAGCATTTCGCTGCTGGCCAGTTGCTCGGCCAGTTCGAGCATGGCGGCATCCTGCGGGTCGCTGCCCAGTTGCTCGCGCTCCTCGACCAACTTGCGCTgg > 1:1143282/1‑236 (MQ=255) gCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCGCAGAGCTGCAGTTCTTCCAGCAGCATTTCGCTGCTGGCCAGTTGCTCGGCCAGTTCGAGCATGGCGGCATCCTGCGGGTCGCTGCCCAGTTGCTCGCGCTCCTCGACCAACTTGCGCTg < 1:588011/235‑1 (MQ=255) ccAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCGCAGAGCTGCAGTTCTTCCAGCAGCATTTCGCTGCTGGCCAGTTGCTCGGCCAGTTCGAGCATGGCGGCATCCTGCGGGTCGCTGCCCAGTTGCTCGCGCTCCTCGACCAACTTGCGCTGGCGCTCg < 1:1186620/236‑1 (MQ=255) cAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCGCAGAGCTGCAGTTCTTCCAGCAGCATTTCGCTGCTGGCCAGTTGCTCGGCCAGTTCGAGCATGGCGGCATCCTGCGGGTCGCTGCCCAGTTGCTcgcg > 2:935266/1‑207 (MQ=255) aGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCGCAGAGCTGCAGTTCTTCCAGCAGCATTTCGCTGCTGGCCAGTTGCTCGGCCAGTTCGAGCATGGCGGCATCCTGCGGGTCGCTGCCCAGTTGCTCGCGCTCCTCGACCAACTTGCGCTGGCGCTCGGc < 1:1086128/236‑1 (MQ=255) aGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCGCAGAGCTGCAGTTCTTCCAGCAGCATTTCGCTGCTGGCCAGTTGCTCGGCCAGTTCGAGCATGGCGGCATCCTGCGGGTCGCTGCCCAGTTGCTCGCGCTCCTCGACCAACTTGCGCTGGCGCTCGGc < 1:628865/236‑1 (MQ=255) ccacccaGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCGCAGAGCTGCAGTTCTTCCAGCAGCATTTCGCTGCTGGCCAGTTGCTCGGCCAGTTCGAGCATGGCGGCATCCTGCGGGTCGCTGCCCAGTTGCTCGCGCTCCTCGACCAACTTGCGCTGGCGCTCGGCCTGgc < 1:1270004/235‑1 (MQ=255) cacccaGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCGCAGAGCTGCAGTTCTTCCAGCAGCATTTCGCTGCTGGCCAGTTGCTCGGCCAGTTCGAGCATGGCGGCATCCTGCGGGTCGCTGCCCAGTTGCTCGCGCTCCTCGACCAACTTGCGCTGGCGCTCGGCCTGgcg > 1:301459/1‑235 (MQ=255) gcTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCGCAGAGCTGCAGTTCTTCCAGCAGCATTTCGCTGCTGGCCAGTTGCTCGGCCAGTTCGAGCATGGCGGCATCCTg > 2:521998/1‑166 (MQ=255) gcTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCCCTTCGCAGAGCTGCAGTTCTTCCAGCAGCATTTCGCTGCTGGCCAg > 2:1233902/1‑133 (MQ=255) | TCCAGGCCGGCAAAATCAAGGTCGTTGAAATCGTCCACCAGCACAGCCTGCAGATCGGCGCCAAGCACCGTCTCTACCGCCAGCTCCCAACCCGGCTCCACCCGCAAGCCTTCGGCCAGCCGCGGCTGTTGCTCCAGCCCTTGGCCGTGCAGCCATTGCGCAGCACCTGCGCCCGGCTCCAGGGCGGCCTGCTGCAAGGCTTCCAGCGAGGCCAGGCGCCCACCCAGGCGCTGCAGGTCGCCCTGTGCCTGCTGTTGCGCCTGGGTGGCCTGCTGCAGTTGCTCGCGCGCGCTTTCCAGGCGCTCGATCACCTGCTCTTCGCAGAGCTGCAGTTCTTCCAGCAGCATTTCGCTGCTGGCCAGTTGCTCGGCCAGTTCGAGCATGGCGGCATCCTGCGGGTCGCTGCCCAGTTGCTCGCGCTCCTCGACCAACTTGCGCTGGCGCTCGGCCTGGCG > AE015451/4864179‑4864633 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |