Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	AE015451	196,327	A→C	10.0%	T611T (ACA→ACC)	PP_0168 →	putative surface adhesion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	196,327	0	A	C	10.0%	148.4 / 2.2	61	T611T (ACA→ACC)	PP_0168	putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base A (29/25);  new base C (3/4);  total (32/29)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

CCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACGGTCAGCGTCACCATCGAGAACGCCACGGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGA  >  AE015451/196093‑196541                                                                                                                                                                                                                                           |                                                                                                                                                                                                                       ccAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAg                                                                                                                                                                                                                       <  1:4333/236‑1 (MQ=18)          ggCCAATGACGTCTACAACAACGGTTCGACGGTCAGCGTCACCATCGAGAACGCCACGGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGt                                                                                                                                                                                                              <  1:919101/236‑1 (MQ=255)               aTGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAAc                                                                                                                                                                                                         <  1:745855/236‑1 (MQ=255)                        acaacaACGGTTCGACGGTCAGCGTCACCATCGAGAACGCCACGGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAAc                                                                                                                                                                                                 <  1:1117405/235‑1 (MQ=17)                                              cGTCACCATCGAGAACGCCACGGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACTGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTg                                                                                                                                                                                                                     <  2:842241/193‑1 (MQ=255)                                               gTCACCATCGAGAACGCCACGGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCg                                                                                                                                                                                <  1:107142/229‑1 (MQ=17)                                                         agaACGCCACGGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGc                                                                                                                                                                              >  1:767523/1‑221 (MQ=39)                                                              gCCACGGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACATACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCGAACGGCCAAACCATTACCGTTGAAGCCGGCACAACCAAGGGCa                                                                                                                                                                 >  1:22657/1‑229 (MQ=255)                                                                cACGGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAAc                                                                                                                                                                                                         >  1:435663/1‑187 (MQ=38)                                                                           acttCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCGGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCg                                                                                                                                                                                <  2:736685/199‑1 (MQ=21)                                                                                 aGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGGCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGa                                                                                                                                         <  1:831679/234‑1 (MQ=16)                                                                                    aaCTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGt                                                                                                                                                                                                              >  2:691512/1‑162 (MQ=25)                                                                                    aaCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGt                                                                                                                                                                                                              <  1:691515/162‑1 (MQ=40)                                                                                     aCTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGCGCAACCCTGCCCAGACGCCGGTGACCGTGACCCAGTCCAACGGCCAAACCATTACCGTTGAAGCCg                                                                                                                                                                                <  2:493616/191‑1 (MQ=255)                                                                                       tGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCg                                                                                                                                                                                <  2:910335/189‑1 (MQ=18)                                                                                         aCCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAAc                                                                                                                                                                                                         <  1:1143878/162‑1 (MQ=25)                                                                                         aCCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAAc                                                                                                                                                                                                         >  2:1143875/1‑162 (MQ=18)                                                                                         aCCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCg                                                                                                                                                                                <  2:536659/187‑1 (MQ=18)                                                                                                 cccGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGACCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGa                                                                                                                                                                                     <  1:298036/174‑1 (MQ=38)                                                                                                  ccGACGCCGGCGCAGACCACGCTCAACGACTCGGTCGACACCACGACCGCGACCCTCACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAg                                                                                                                                                                                                                       <  2:375157/139‑1 (MQ=255)                                                                                                   cGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCCGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACg                                                                                                                       >  2:939381/1‑234 (MQ=12)                                                                                                      cGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCg                                                                                                                                                                                <  1:325101/174‑1 (MQ=38)                                                                                                         cGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCg                                                                                                                                                                                <  1:365003/171‑1 (MQ=18)                                                                                                            cTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCCACGGCCACAcctccac                                                                                                                                                                                           >  1:603740/1‑152 (MQ=255)                                                                                                                   cACGATCAACGGCTCGGTCGACACCCCCACCGCGCCCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTCATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAAc                                                                                                                                                                                                 <  1:263042/144‑1 (MQ=11)                                                                                                                        tCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCAt                                                                                                 >  1:429796/1‑235 (MQ=14)                                                                                                                        tCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGAc                                                                                                                                                                                                                   >  2:1230747/1‑121 (MQ=255)                                                                                                                                tctgTCGACCCCCCCACCGCCACCCTGACGGCGCGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAAc                                                                                                                                                                                                         <  2:193678/120‑1 (MQ=11)                                                                                                                                     cGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTg                                                                                                                                                                                                                     <  2:887468/106‑1 (MQ=25)                                                                                                                                               aCCGCGACCCTGACGGCGAGCCCGTCGGACACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCa                                                                                                                                                                                                    <  2:96458/113‑1 (MQ=17)                                                                                                                                                 cgcgACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACAGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACaacaac                                                                                                                        >  1:1124166/1‑187 (MQ=18)                                                                                                                                                   cgACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCa                                                                                                                                                                      >  1:361560/1‑139 (MQ=34)                                                                                                                                                      cccTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCa                                                                                                           >  1:1263342/1‑195 (MQ=14)                                                                                                                                                                    ccGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGCCGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACaggcgg                                                                               >  2:1137430/1‑209 (MQ=11)                                                                                                                                                                        cGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCgc                                                                                                                                                             >  1:1030860/1‑126 (MQ=255)                                                                                                                                                                               cGAAGGCGGCGTAATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAAc                                                                                                                                                                                                         <  2:104717/76‑1 (MQ=21)                                                                                                                                                                                   ggcggcGAGATCTCTTACACCCTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGt                                                                                                                                                                                                              <  1:1018751/67‑1 (MQ=11)                                                                                                                                                                                    gcggcgTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGaa                                                                                           >  1:168361/1‑181 (MQ=37)                                                                                                                                                                                           gATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGCACGCCACaggcggg                                                                              >  1:380442/1‑186 (MQ=11)                                                                                                                                                                                                   tccccGTGACCCTGAGCAATCCTGCCCAGCCGCCGGTGCCAGTGCCCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGAAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTacaa                                                                                                                            <  1:211232/130‑1 (MQ=11)                                                                                                                                                                                                            cccTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTac                                                                                                                              >  1:365063/1‑122 (MQ=32)                                                                                                                                                                                                            cccTGAGCAATCCTGCCCAGACGCCGGTAACAGTGACCCTGCCCTACGACCCAACCATTACCGTTCAAGCCGGCAAAACCCAGGGCAg                                                                                                                                                                >  1:417151/1‑88 (MQ=11)                                                                                                                                                                                                              cTGAGCAATCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGAc                                                                                                                                    <  2:1105472/114‑1 (MQ=12)                                                                                                                                                                                                              cTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTAGAGCAAc                                                                 >  1:101458/1‑181 (MQ=37)                                                                                                                                                                                                              cTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTCCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGGGTGTCAg                                                                                                          >  1:1091229/1‑140 (MQ=255)                                                                                                                                                                                                                 aGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTac                                                                                                                              <  2:1279584/117‑1 (MQ=12)                                                                                                                                                                                                                 aGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTac                                                                                                                              >  1:1279587/1‑117 (MQ=17)                                                                                                                                                                                                                  gCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCGGCGTCGATTTCCAGACCCCGGCGAATGACGCCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGGAATTTc                                                                        >  1:308983/1‑170 (MQ=255)                                                                                                                                                                                                                  gCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACcggcg                                                                                >  1:311903/1‑162 (MQ=38)                                                                                                                                                                                                                  gCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCcaccac       >  1:1096293/1‑235 (MQ=39)                                                                                                                                                                                                                    aaTCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGACTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCAc                                                                                     >  1:224555/1‑155 (MQ=25)                                                                                                                                                                                                                      tCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGa  >  1:876372/1‑236 (MQ=38)                                                                                                                                                                                                                         tGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGa                                                                                                                                                  <  2:9255/89‑1 (MQ=33)                                                                                                                                                                                                                         tGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGa                                                                                                                                                  >  1:9255/1‑89 (MQ=33)                                                                                                                                                                                                                         tGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCAAGAAAca                                                                >  1:43047/1‑170 (MQ=16)                                                                                                                                                                                                                                   ccGGTGACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGcca                                                                                                                                         <  2:639220/88‑3 (MQ=16)                                                                                                                                                                                                                                   ccGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCa                                                                                                                                                                 >  1:906035/1‑64 (MQ=35)                                                                                                                                                                                                                                   ccGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAt                                                                                                                                       <  2:276748/90‑1 (MQ=33)                                                                                                                                                                                                                                   ccGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAt                                                                                                                                       >  1:276749/1‑90 (MQ=33)                                                                                                                                                                                                                                   ccGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACAGTCAGTGTCACCATCGAGAACGCCACaggcg                                                                                >  2:118686/1‑145 (MQ=17)                                                                                                                                                                                                                                        gACAGTGACCCTGTCCAACGGCCAAACCATTACCGCTGTCGCCTATACAACCCAGCGCACCGACGAGTCCCAGGCCCCGCCCAATGACCTCCTCAACAACgg                                                                                                                      >  1:776081/1‑102 (MQ=2)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                       CCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACGGTCAGCGTCACCATCGAGAACGCCACGGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGA  >  AE015451/196093‑196541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑