Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	AE015451	196,390	T→C	9.0%	D632D (GAT→GAC)	PP_0168 →	putative surface adhesion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	196,390	0	T	C	9.0%	136.5 / 4.5	54	D632D (GAT→GAC)	PP_0168	putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base T (31/17);  new base C (3/2);  total (35/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

AGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGG  >  AE015451/196173‑196622                                                                                                                                                                                                                          |                                                                                                                                                                                                                                         aGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGGCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGa                                                                                                                                                                                                                          <  1:831679/234‑1 (MQ=16)                   cGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCCGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACg                                                                                                                                                                                                        >  2:939381/1‑234 (MQ=12)                                        tCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCAt                                                                                                                                                                                  >  1:429796/1‑235 (MQ=14)                                                                 cgcgACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACAGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACaacaac                                                                                                                                                                                                         >  1:1124166/1‑187 (MQ=18)                                                                      cccTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCa                                                                                                                                                                                            >  1:1263342/1‑195 (MQ=14)                                                                                    ccGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGCCGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACaggcgg                                                                                                                                                                >  2:1137430/1‑209 (MQ=11)                                                                                                    gcggcgTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGaa                                                                                                                                                                            >  1:168361/1‑181 (MQ=37)                                                                                                           gATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGCACGCCACaggcggg                                                                                                                                                               >  1:380442/1‑186 (MQ=11)                                                                                                                   tccccGTGACCCTGAGCAATCCTGCCCAGCCGCCGGTGCCAGTGCCCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGAAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTacaa                                                                                                                                                                                                             <  1:211232/130‑1 (MQ=11)                                                                                                                            cccTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTac                                                                                                                                                                                                               >  1:365063/1‑122 (MQ=32)                                                                                                                              cTGAGCAATCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGAc                                                                                                                                                                                                                     <  2:1105472/114‑1 (MQ=12)                                                                                                                              cTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTAGAGCAAc                                                                                                                                                  >  1:101458/1‑181 (MQ=37)                                                                                                                              cTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTCCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGGGTGTCAg                                                                                                                                                                                           >  1:1091229/1‑140 (MQ=255)                                                                                                                                 aGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTac                                                                                                                                                                                                               <  2:1279584/117‑1 (MQ=12)                                                                                                                                 aGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTac                                                                                                                                                                                                               >  1:1279587/1‑117 (MQ=17)                                                                                                                                  gCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCGGCGTCGATTTCCAGACCCCGGCGAATGACGCCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGGAATTTc                                                                                                                                                         >  1:308983/1‑170 (MQ=255)                                                                                                                                  gCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACcggcg                                                                                                                                                                 >  1:311903/1‑162 (MQ=38)                                                                                                                                  gCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCcaccac                                                                                        >  1:1096293/1‑235 (MQ=39)                                                                                                                                    aaTCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGACTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCAc                                                                                                                                                                      >  1:224555/1‑155 (MQ=25)                                                                                                                                      tCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGa                                                                                   >  1:876372/1‑236 (MQ=38)                                                                                                                                         tGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGa                                                                                                                                                                                                                                   >  1:9255/1‑89 (MQ=33)                                                                                                                                         tGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGa                                                                                                                                                                                                                                   <  2:9255/89‑1 (MQ=33)                                                                                                                                         tGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCAAGAAAca                                                                                                                                                 >  1:43047/1‑170 (MQ=16)                                                                                                                                                   ccGGTGACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGcca                                                                                                                                                                                                                          <  2:639220/88‑3 (MQ=16)                                                                                                                                                   ccGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAt                                                                                                                                                                                                                        >  1:276749/1‑90 (MQ=33)                                                                                                                                                   ccGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAt                                                                                                                                                                                                                        <  2:276748/90‑1 (MQ=33)                                                                                                                                                   ccGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACAGTCAGTGTCACCATCGAGAACGCCACaggcg                                                                                                                                                                 >  2:118686/1‑145 (MQ=17)                                                                                                                                                        gaccgTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAt                                                                                                                                                                                                                        >  1:1268340/5‑85 (MQ=0)                                                                                                                                                        gACAGTGACCCTGTCCAACGGCCAAACCATTACCGCTGTCGCCTATACAACCCAGCGCACCGACGAGTCCCAGGCCCCGCCCAATGACCTCCTCAACAACgg                                                                                                                                                                                                       >  1:776081/1‑102 (MQ=2)                                                                                                                                                           tgTGCCCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCAGACCACGATCAACg                                                                                                           <  2:1234658/190‑1 (MQ=255)                                                                                                                                                           tgTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCa                                                                                                                         <  2:1113716/176‑1 (MQ=38)                                                                                                                                                           ggTGAACCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCTAAACCCAGTGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCCACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGCCCCCGAATCCg                                                                                                                                    <  1:377328/165‑1 (MQ=255)                                                                                                                                                                      ccAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAAt                                                                                                                                                                                                                        <  2:859828/71‑1 (MQ=255)                                                                                                                                                                        aaCGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCTTCGACATCCAGAccc                                                                                                                                                                                                                                >  2:1055153/1‑61 (MQ=11)                                                                                                                                                                        aaCGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGa                                                                                                                                                                                                                          >  1:953201/1‑67 (MQ=35)                                                                                                                                                                        aaCGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTa                                                                                                                                                              >  1:133117/1‑127 (MQ=38)                                                                                                                                                                        aaCGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCg                                                                                                                                    <  2:1195568/153‑1 (MQ=39)                                                                                                                                                                        aaCGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCg                                                                                                                                    >  1:1195571/1‑153 (MQ=39)                                                                                                                                                                         aCGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc                                                <  1:1091524/236‑1 (MQ=21)                                                                                                                                                                          cGGCCAAACCATTACCGTTGAAGCGGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAGCGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc                                                <  1:982899/235‑1 (MQ=9)                                                                                                                                                                                  ccATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCAc                                        >  1:459348/1‑235 (MQ=39)                                                                                                                                                                                  ccATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGCAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCAGACCACGATCAACCACTCGGGCGACGCCACCACCGCGAcccc                                                                               >  1:278792/1‑195 (MQ=255)                                                                                                                                                                                     gtACCGCTGAAGCCGGCAAAGCCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACGGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCAGACCACGATCAACg                                                                                                           <  2:181267/164‑1 (MQ=255)                                                                                                                                                                                     acaCCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAt                                                                                                                                                                                                                        <  2:226700/54‑1 (MQ=25)                                                                                                                                                                                           ttGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGTTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTa                                                                                                                                                              <  2:1124163/108‑1 (MQ=255)                                                                                                                                                                                                gCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACg                                                                                                                                                                                                        >  1:564746/1‑61 (MQ=35)                                                                                                                                                                                                   ggCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACAGGCGGTAATTTc                                                                                                                                                         >  1:866334/1‑105 (MQ=17)                                                                                                                                                                                                      aaaaCCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAg                                                                                                                                                                                           >  1:648976/1‑68 (MQ=35)                                                                                                                                                                                                                gCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCt                         <  2:1096290/220‑1 (MQ=39)                                                                                                                                                                                                                 cAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGAGTCACCATCGAGAACGCCACCGGCGGCAAATTCGAGc                                                                                                                                                     >  2:201106/1‑95 (MQ=255)                                                                                                                                                                                                                      tCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGgcggcg                                               >  1:50698/1‑192 (MQ=37)                                                                                                                                                                                                                       cGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGa                                                                                                                       >  1:425187/1‑119 (MQ=25)                                                                                                                                                                                                                       cGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGCAATTTTGa                                                                                                                                                       >  2:297028/1‑87 (MQ=255)                                                                                                                                                                                                                        gATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCgg  <  1:482074/235‑1 (MQ=37)                                                                                                                                                                                                                          |                                                                                                                                                                                                                                         AGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGG  >  AE015451/196173‑196622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑