Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	AE015451	195,505	C→G	8.6%	A337A (GCC→GCG)	PP_0168 →	putative surface adhesion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	195,505	0	C	G	8.6%	126.9 / 3.1	45	A337A (GCC→GCG)	PP_0168	putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base C (20/21);  new base G (2/2);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGACGGTGACATTGCCGCAAACGCCAGCGGTTACCCAAGTGACCGATACAATCGACACTACAACCGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGTGGCGTGATCACCTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACAC  >  AE015451/195273‑195690                                                                                                                                                                                                                                         |                                                                                                                                                                                          tGACGGTGACATTGCCGCAAACGCCAGCGGTTACCCAAGTGACCGATACAATCGACACTACAACCGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGTGGCGTGATCACCTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCa                                                                                                                                                                                          <  2:507647/234‑1 (MQ=255)              gCCGCAAACGCCAGCGGTTACCCAAGTGACCGATACAATCGACACTACAACCGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGTGGCGTGATCACCTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGcc                                                                                                                                                                                           <  2:884435/220‑1 (MQ=255)                cGCAAACGCCAGCGGTTACCCAAGTGACCGATACAATCGACACTACAACCGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGTGGCGTGATCACCTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCa                                                                                                                                                                                          <  2:1108043/219‑1 (MQ=255)                                         gACCGATACAATCGACACTACAACCGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGTGGCGTGATCACCTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAAt                                                                                                                                                                                        <  1:881547/196‑1 (MQ=11)                                          aCCGATACAATCGACACTACAACCGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGTGGCGTGATCACCTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCg                                                                                                                                                >  1:691634/1‑235 (MQ=255)                                                aCAATCGACACTACAACCGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGTGGCGTGATCACCTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGCCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGc                                                                                                                                         >  1:431879/1‑236 (MQ=255)                                                   aTCGACACTACAACCGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGTGGCGTGATCACCTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGtt                                                                                                                                                                     <  2:806792/205‑1 (MQ=11)                                                                    gACCCTGACCGCCTCGCCAAGCGTGACCGAAGGTGGCGTGATCACCTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAg                                                                                                                                                           <  2:866193/198‑1 (MQ=255)                                                                                  cGCCAAGCGTGACCGAAGGTGGCGTGATCACCTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGaa                                                                                                        <  2:610834/235‑1 (MQ=255)                                                                                     cAAGCGTGACCGAAGGTGGCGTGATCACCTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCa                                                                                                                                                                                          >  1:856523/1‑150 (MQ=255)                                                                                     cAAGCGTGACCGAAGGTGGCGTGATCACCTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCa                                                                                                                                                                                          <  2:856520/150‑1 (MQ=255)                                                                                                  aGGTGGCGTGATCACCTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGa                                                                                       <  1:463744/236‑1 (MQ=255)                                                                                                               aCCTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGCCCGTCAGCgc                                                                                                                                                        >  1:56476/1‑157 (MQ=18)                                                                                                                          gACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGa                                                                          >  2:894643/1‑225 (MQ=255)                                                                                                                            cccTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCg                                                                                                          >  1:515506/1‑191 (MQ=16)                                                                                                                              cTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGACCGCCACcggcggca                                                                                                                              >  1:126274/1‑167 (MQ=17)                                                                                                                              cTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACcggcggc                                                                                                                               >  2:472240/1‑167 (MQ=18)                                                                                                                              cTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCAACACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCAcc                                                                                                                                     >  1:342096/1‑162 (MQ=18)                                                                                                                              cTGAGCAACCCTGCCCAGACGCCGGTGACCGTGAACCTGTCCAACGGCCAAGCCATCACCGTTGAAGCTGGCAACTGCACCGACAGCCTCGATCTCCAGACTCCCGCGAATGACGtatg                                                                                                                                                                                >  1:1202705/1‑116 (MQ=2)                                                                                                                                    aaCCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCgaga                                                                                                                                             >  1:946531/1‑148 (MQ=34)                                                                                                                                    aaCCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAGCGCCACcggcggca                                                                                                                              >  1:459338/1‑161 (MQ=17)                                                                                                                                    aaCCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAACTTCg                                                                                                                        >  1:401937/1‑169 (MQ=18)                                                                                                                                              aGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCGGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGGCTACAACAACGGTTCGACCGCCAGCGTCACCATCGAGAAAGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGcc                                                                                           >  1:25499/1‑187 (MQ=255)                                                                                                                                              aGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGt                                                                                                                                                              <  2:1135567/121‑1 (MQ=33)                                                                                                                                              aGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGt                                                                                                                                                              >  1:1135570/1‑121 (MQ=33)                                                                                                                                                        gaccgtgaccCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCg                                        >  1:578142/1‑229 (MQ=255)                                                                                                                                                           cgtgaccCTGCCCAGCGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACg                                                                           <  2:269346/191‑1 (MQ=255)                                                                                                                                                                     tCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAg                     <  1:1271469/235‑1 (MQ=21)                                                                                                                                                                       cAACGGCCAGGTCATCACTGTTGAAGCCCGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcg                  <  1:1103061/236‑1 (MQ=255)                                                                                                                                                                         aCGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc                <  1:386279/236‑1 (MQ=255)                                                                                                                                                                           ggCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGt              <  1:894646/236‑1 (MQ=255)                                                                                                                                                                                 gTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCAc        <  1:1108144/236‑1 (MQ=255)                                                                                                                                                                                       aCTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc                <  1:478470/222‑1 (MQ=40)                                                                                                                                                                                         cgTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGaa                      >  1:493619/2‑214 (MQ=2)                                                                                                                                                                                             gAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAACTTCGAGCAACTGACCCCGAAACCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACGCCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACtt      <  1:398776/226‑1 (MQ=255)                                                                                                                                                                                                 gcGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAGCTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCg                                                                      <  2:121674/157‑1 (MQ=9)                                                                                                                                                                                                   ggCAAAACCCA‑GGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTacac  <  1:96982/223‑1 (MQ=255)                                                                                                                                                                                                     cAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggc                   >  1:912026/1‑205 (MQ=38)                                                                                                                                                                                                      aaaaCCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc                >  1:853492/1‑207 (MQ=21)                                                                                                                                                                                                      aaaaCCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGCCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAc                                                                            <  2:222262/147‑1 (MQ=37)                                                                                                                                                                                                       aaaCCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcggc                <  1:1150054/206‑1 (MQ=21)                                                                                                                                                                                                        aaCCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGCAATTTCGAgg                                                                                                                     >  1:183094/1‑103 (MQ=11)                                                                                                                                                                                                             aGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCAc        <  1:183057/208‑1 (MQ=14)                                                                                                                                                                                                                 cAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGGCGACACCACCACCGCGACCCTGACGGCg                                        >  1:334153/1‑172 (MQ=38)                                                                                                                                                                                                                       cGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCt                                               >  1:129428/1‑159 (MQ=25)                                                                                                                                                                                                                        gagttCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCAc        <  1:149905/194‑1 (MQ=9)                                                                                                                                                                                                                                         cAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGAcc                                                                                     <  2:846943/103‑1 (MQ=33)                                                                                                                                                                                                                                         cAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGAcc                                                                                     >  1:846946/1‑103 (MQ=35)                                                                                                                                                                                                                                         |                                                                                                                                                                                          TGACGGTGACATTGCCGCAAACGCCAGCGGTTACCCAAGTGACCGATACAATCGACACTACAACCGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGTGGCGTGATCACCTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACAC  >  AE015451/195273‑195690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑