| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 199,744 | T→C | 8.9% | N1750N (AAT→AAC) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 199,744 | 0 | T | C | 8.9% | 240.1 / 7.8 | 76 | N1750N (AAT→AAC) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base T (38/31); new base C (4/3); total (42/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGC > AE015451/199512‑199972 | aaGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAAc > 1:1098179/1‑236 (MQ=40) tGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCgg < 1:1120542/236‑1 (MQ=255) aCGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGa < 1:531552/235‑1 (MQ=255) cGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACt > 1:1168167/1‑230 (MQ=255) cAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGAcc < 2:1098176/234‑1 (MQ=40) aTCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGa < 1:90035/212‑1 (MQ=40) gggCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAACGACGGTCTGCCGATCAACAACCACt > 2:832535/1‑200 (MQ=255) tCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTCGAGTTGGGCGGTGCTGCTACGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCGTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGa < 2:668801/199‑1 (MQ=255) accTCGGCGGTGGACGCCCCTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGtcgtcg < 1:209656/233‑1 (MQ=255) aaCTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGtcgtcg < 1:1055741/235‑1 (MQ=40) ggcggTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGa < 2:72387/192‑1 (MQ=37) gcggTGGCCGCCACTGGTGCTGCATTCGAGAACCCTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGc < 1:337888/236‑1 (MQ=255) gCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGtcgtcg > 2:1258086/1‑220 (MQ=38) gCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCgtcg > 2:880088/1‑217 (MQ=14) gCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCgtcg < 1:880091/217‑1 (MQ=11) ctgcATTCGAGAACCTTGAGCTGGGCGGTGCTGCGTCGGTGCAAGTCACCGACACCCCCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCGTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAAcc < 2:377242/165‑1 (MQ=255) gcATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCa > 2:1044603/1‑181 (MQ=38) aTTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGc < 2:419285/234‑1 (MQ=40) ttCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGcaccac > 1:79791/1‑197 (MQ=34) aaCCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGAc > 2:813762/1‑183 (MQ=37) tGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGtcgtcg > 2:528280/1‑194 (MQ=37) aGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCa < 1:746913/236‑1 (MQ=255) gCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGAc > 2:746910/1‑222 (MQ=40) cTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAg < 1:481513/235‑1 (MQ=40) ggTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGcaccaccg > 2:962879/1‑203 (MQ=14) gTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTg < 2:1283241/161‑1 (MQ=35) ttCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTg < 1:812390/173‑1 (MQ=34) tCACCGACACCCTCGACGAGGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACt < 2:1135503/123‑1 (MQ=18) aCCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAAcc < 2:793312/201‑1 (MQ=40) cccTCGACGAAGTGGTGGCCAAGCTGACTGCCACTCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAAc < 2:840250/193‑1 (MQ=255) gacgaGGTGGTGGCCAAGCTGACTGCCACTCCGTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCCACCGCACCAGACAACGTCTATGTCGGCACCAAcga > 1:763702/1‑220 (MQ=255) acgaAGTGGTGGCCAAGCTGACTGCCCCCCCTTCGGTGCCCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCa < 1:346190/194‑1 (MQ=255) aGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTa < 1:1283655/201‑1 (MQ=38) tggtggCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGAc < 1:412714/236‑1 (MQ=40) ggCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCa > 2:884860/1‑206 (MQ=40) ggCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCa < 1:884863/206‑1 (MQ=40) aaGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATc > 1:1086949/1‑225 (MQ=39) aaGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAg > 1:550792/1‑236 (MQ=40) aaGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGCCTATGTAGGCACCAACGCGCCgg > 1:1047005/1‑211 (MQ=255) gatctcaccgccTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGGCAgcg > 2:804816/11‑233 (MQ=255) gACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAgcggc > 2:877651/1‑235 (MQ=40) cgccTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTg > 1:540343/3‑117 (MQ=21) cgtCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCa < 1:290475/225‑1 (MQ=39) cTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAAc > 2:977100/1‑157 (MQ=255) cGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAAAAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGc > 1:310781/1‑179 (MQ=39) ccGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACg > 2:445878/1‑131 (MQ=34) gAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAACGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACaa > 2:46211/1‑158 (MQ=25) aGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCACGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCCCCACGGCCTCGGCGACTg > 2:65261/1‑140 (MQ=255) aCCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACa > 1:63177/1‑235 (MQ=40) cacCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGCTGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGaaa < 2:771461/215‑1 (MQ=255) cacCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGtt > 1:78071/1‑210 (MQ=40) cacgcTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCa > 2:564693/5‑149 (MQ=255) cACGCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCa < 1:564696/149‑1 (MQ=255) cccTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAAc > 2:246158/1‑131 (MQ=37) cccTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGt > 1:991217/1‑157 (MQ=40) ccTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAg > 1:969822/1‑223 (MQ=255) ccTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAg < 2:969819/223‑1 (MQ=255) cTGACCAACAAAGATGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATg > 1:1002804/1‑190 (MQ=21) cTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACTACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCa > 2:1086725/1‑145 (MQ=255) cTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCa < 1:1086728/145‑1 (MQ=40) cTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCa > 1:847623/1‑166 (MQ=255) cTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCa < 2:847620/166‑1 (MQ=255) ccAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAaacct > 1:584594/1‑205 (MQ=255) aacaaAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTTGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGt > 1:722525/1‑149 (MQ=255) aacaaAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGAt < 1:140012/152‑1 (MQ=255) aacaaAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGAt > 2:140012/1‑152 (MQ=255) aacaaAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACa > 2:646738/1‑214 (MQ=255) aacaaAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAgg > 1:520609/1‑235 (MQ=255) aacaaAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAACCAACAGCACCACGGGCTCGGCGACTGCaa > 2:1049927/1‑109 (MQ=255) caaGACGGTCTGCCGATCAATATCCACGCCGAGCTGTGCTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCACCAGCCCCACGCGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAaacctg < 1:302420/200‑1 (MQ=14) aaGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAaacctgaac > 2:703489/1‑203 (MQ=255) tCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGc < 1:804819/236‑1 (MQ=255) tCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGc < 1:1163479/236‑1 (MQ=255) ccGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGGCACCGACGAGCCAgg > 1:207509/1‑236 (MQ=255) cGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGcaccac > 1:1156117/1‑76 (MQ=35) cGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTGACCGACGAGCCAGGc < 1:827811/236‑1 (MQ=25) | AAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGC > AE015451/199512‑199972 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |