Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	AE015451	1,530,173	A→C	100%	*399S (TAA→TCA)  noncoding (62/244 nt)	ftsZ → PP_mr19 →	Cell division protein FtsZ Xoo8 ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	1,530,173	0	A	C	95.0%	290.1 / 5.3	80	*399S (TAA→TCA)  noncoding (62/244 nt)	ftsZ PP_mr19	Cell division protein FtsZ Xoo8 ncRNA
Reads supporting (aligned to +/- strand):  ref base A (0/4);  new base C (24/52);  total (24/56)
Fisher's exact test for biased strand distribution p-value = 3.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.22e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTGAAGGTGGTCGACAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAA  >  AE015451/1529947‑1530372                                                                                                                                                                                                                                   |                                                                                                                                                                                                        gtgAAGGTGGTCGACAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAAttt                                                                                                                                                                                                <  1:1059242/236‑1 (MQ=255)  tgAAGGTGGTCGACAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAAt                                                                                                                                                                                                  >  2:1093510/1‑233 (MQ=255)  tgAAGGTGGTCGACAATACCCTGCAGACTGCCCAGCA‑GCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTAt                                                                                                                                                                                              <  1:637602/236‑1 (MQ=255)    aaGGTGGTCGACAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTa                                                                                                                                                                                               <  2:867459/234‑1 (MQ=255)    aaGGTGGTCGACAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTAt                                                                                                                                                                                              <  1:1093513/235‑1 (MQ=255)             gACAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGtata                                                                                                                                                                                    >  1:814676/1‑236 (MQ=255)             gACAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGtata                                                                                                                                                                                    >  1:1007567/1‑236 (MQ=255)               cAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAAttt                                                                                                                                                                                                <  2:1007564/222‑1 (MQ=255)                          aGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGTCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTg                                                                                                                                                                          <  2:1212888/233‑1 (MQ=255)                            aCTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTAt                                                                                                                                                                      >  1:1077893/1‑235 (MQ=255)                                cccAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGCAGCGCCCGACCGTCATGCTCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTg                                                                                                                                                                          <  1:1209566/227‑1 (MQ=255)                                                 gCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGt                                                                                                                                                  <  1:806151/234‑1 (MQ=255)                                                   gcccAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTg                                                                                                                                                         <  1:649394/223‑1 (MQ=255)                                                      cAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTAt                                                                                                                                                                                              <  2:492612/185‑1 (MQ=255)                                                      cAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTatc                                                                                                                                              >  2:1071122/1‑233 (MQ=255)                                                       aacccTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCaaa                                                                                                                                                              <  1:898634/213‑1 (MQ=255)                                                       aaTCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATgg                                                                                                                                                                                                     >  1:635472/1‑177 (MQ=255)                                                       aaTCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCGGGGGt                                                                                                                                                                                       >  1:950302/1‑191 (MQ=255)                                                           cTGCGCCGGTGCGCCAGGAGCGGCCCGCGGTCAACTACCGGGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTAt                                                                                                                                                                                              <  2:271582/180‑1 (MQ=255)                                                           cTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAg                                                                                                                                      <  1:610505/236‑1 (MQ=255)                                                            tGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTAt                                                                                                                                                                                              <  2:824226/179‑1 (MQ=255)                                                                ccGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAg                                                                                                                                      <  1:949788/231‑1 (MQ=255)                                                                 cGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATcc                                                                                                                                          >  1:383492/1‑226 (MQ=255)                                                                 cGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCt                                                                                                                                   >  2:821117/1‑233 (MQ=255)                                                                             agcagcCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTAt                                                                                                                                                                                              <  1:609266/162‑1 (MQ=255)                                                                               cagccagcGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAAtt                                                                                                                                                                                                 <  2:1230158/157‑1 (MQ=255)                                                                                  ccagcGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGc                                                                                                                 <  2:398670/234‑1 (MQ=255)                                                                                              tACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTAt                                                                                                                                                                                              <  2:241634/145‑1 (MQ=255)                                                                                                   tGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTttgttg                                                                                                                             <  2:1021116/205‑1 (MQ=255)                                                                                                   tGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCgg                                                                                                >  1:260909/1‑234 (MQ=255)                                                                                                     aCCTGGCGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCaa                                                                                            <  1:215539/236‑1 (MQ=255)                                                                                                     aCCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCaa                                                                                            <  1:433727/236‑1 (MQ=255)                                                                                                        tGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTAt                                                                                                                                                                                              >  1:317838/1‑135 (MQ=255)                                                                                                        tGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTAt                                                                                                                                                                                              <  2:317837/135‑1 (MQ=255)                                                                                                          gAGCGCCCGACCGTCATGCGCAGCCAGGCCCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTTCGTCGTCAGGCTGATTCATGGAAtt                                                                                                                                                                                                 <  1:189852/130‑1 (MQ=255)                                                                                                          gAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAg                                                                                                                                      <  1:179238/189‑1 (MQ=255)                                                                                                                    ccGTCATGCGCAACCAGGCGCATGCAGGTGCTGGCGCAGCCGCTAAACTGAGCCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATg                                                                                                                                                                                                      <  2:750517/115‑1 (MQ=255)                                                                                                                      gTCAGGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAaacaac                                                                           <  1:114927/236‑1 (MQ=255)                                                                                                                         aTGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGTGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGc                                                                         <  1:319638/235‑1 (MQ=255)                                                                                                                          tGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGc                                                                                                  <  1:267431/209‑1 (MQ=255)                                                                                                                          tGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGc                                                                                                  >  2:267430/1‑209 (MQ=255)                                                                                                                           gcgcAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGc                                                                         <  1:4085/233‑1 (MQ=255)                                                                                                                            cgcAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCAcct                                                                     >  2:862330/1‑235 (MQ=255)                                                                                                                             gcAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCt                                                                    <  1:638652/236‑1 (MQ=255)                                                                                                                                  cAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTgaaga                                                               <  1:906167/236‑1 (MQ=255)                                                                                                                                     gcgcATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGc                                                                         <  1:703597/223‑1 (MQ=255)                                                                                                                                       gcATGCAGGTGCTGCCGTAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATAcc                                                          <  1:636059/236‑1 (MQ=255)                                                                                                                                              ggTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTg                                                   >  1:534448/1‑236 (MQ=255)                                                                                                                                               gTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGt                                                    <  1:826233/234‑1 (MQ=255)                                                                                                                                                         cgCCGCTACACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGCAATTTACCAGGGGtat                                                                                                                                                                                     >  2:47610/2‑95 (MQ=255)                                                                                                                                                           ccGCTAAGCTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCg                                       <  1:202854/235‑1 (MQ=255)                                                                                                                                                           ccGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCaaa                                                                                                                                                              <  2:1172106/116‑1 (MQ=255)                                                                                                                                                                 aaCTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCAccc                                                                     <  1:652892/199‑1 (MQ=255)                                                                                                                                                                       aCCCTCAGGATGACCTGGACGACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGtt                                                                                                                                                 <  2:239845/117‑1 (MQ=255)                                                                                                                                                                            cAGGATGACGTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGc                                                  <  1:920680/207‑1 (MQ=255)                                                                                                                                                                              ggATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCg                                                     >  1:49299/1‑202 (MQ=255)                                                                                                                                                                                   aCCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATAcc                                                          <  2:401050/192‑1 (MQ=255)                                                                                                                                                                                    ccTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTa              <  1:1157642/235‑1 (MQ=255)                                                                                                                                                                                    ccTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTAc             <  1:618176/236‑1 (MQ=255)                                                                                                                                                                                     cTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTAcc            >  1:558486/1‑236 (MQ=255)                                                                                                                                                                                            aCCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGGTTACCTGACCCt     >  1:325047/1‑236 (MQ=255)                                                                                                                                                                                              cTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGgt                                           <  2:1058235/196‑1 (MQ=255)                                                                                                                                                                                                cGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGc                                                                         >  2:778904/1‑164 (MQ=255)                                                                                                                                                                                                cGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGc                                                                         <  1:778907/164‑1 (MQ=255)                                                                                                                                                                                                  atatCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCaa  <  2:499872/233‑1 (MQ=255)                                                                                                                                                                                                     tCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCCGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCACCCGTGCcaca                                              >  2:21557/1‑186 (MQ=255)                                                                                                                                                                                                     tCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAATGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAg                                                                                                   >  2:356043/1‑133 (MQ=255)                                                                                                                                                                                                     tCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCaa                                                                                            <  2:373717/140‑1 (MQ=255)                                                                                                                                                                                                        cGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTAc             <  1:1153056/216‑1 (MQ=255)                                                                                                                                                                                                         ggCATTCCTGCGTCGTCGGGCTGATTCATGGAATTTATCAGGGGTACAACGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTttgttg                                                                                                                             <  2:28838/103‑1 (MQ=255)                                                                                                                                                                                                         ggCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGc                                                                         <  2:811103/155‑1 (MQ=255)                                                                                                                                                                                                           cATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGga                       >  2:302282/1‑203 (MQ=255)                                                                                                                                                                                                              tCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCAcc                                                                      >  1:1040670/1‑153 (MQ=255)                                                                                                                                                                                                              tCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCAcc                                                                      <  2:1040667/153‑1 (MQ=255)                                                                                                                                                                                                               ccTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGCTATAAAGGTGATTGGTGTTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACt                              <  2:209045/192‑1 (MQ=255)                                                                                                                                                                                                                 tGCGTCGTCAGTCTTATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATAcc                                                          <  2:134010/162‑1 (MQ=255)                                                                                                                                                                                                                 tGCGTCGTCAGGCGAAGCCATGGAATTTATCAGGGGTATAAAGGTGGTTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCAccc                                                                     <  2:444139/151‑1 (MQ=255)                                                                                                                                                                                                                  gCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGCATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCACAGCCTTTGGTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATAACATCCGTGCCACAGGTGTc                                        >  2:366808/1‑179 (MQ=255)                                                                                                                                                                                                                     tcgtcAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCACCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGAcc       <  2:128582/209‑1 (MQ=255)                                                                                                                                                                                                                          aGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGAc        <  2:555191/203‑1 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                        GTGAAGGTGGTCGACAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAA  >  AE015451/1529947‑1530372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑