Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AE015451 1,617,782 C→G 6.6% intergenic (‑37/+136) PP_1417 ← / ← PP_1418 putative Tricarboxylate transport protein TctB/putative Tricarboxylate transport protein TctC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE0154511,617,7820CG 6.6% 252.7 / 6.2 76intergenic (‑37/+136)PP_1417/PP_1418putative Tricarboxylate transport protein TctB/putative Tricarboxylate transport protein TctC
Reads supporting (aligned to +/- strand):  ref base C (42/28);  new base G (3/2);  total (45/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

GGCGGAACACCAGGTACAGCAGGCCCAGCCCCATCAGGCCGAGCACCAGCAGCGGGTAAGCGCGCGGGCCCACCGGTTCATAGGAGAATGCAGCCTGATAGGGCCAGGCCATCACGGCCAGGGCGGCACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATCCCGACACAAGGCCGCTCCCACAGGAGTGGTGGTGAGTCCGACAGGCTTACTGGATCAGGCCGAATTCCTTGGCCAGCGCCTTGTAGTCAGCCACTTGCTTCTTCACATAGCCGTCCAGCTCTTCGCCGGTCATGGC  >  AE015451/1617547‑1618007
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                 
ggCGGAACACCAGGTACAGCAGGCCCAGCCCCATCAGGCCGAGCACCAGCAGCGGGTAAGCGCGCGGGCCCACCGGTTCATAGGAGAATGCAGCCTGATAGGGCCAGGCCATCACGGCCAGGGCGGCACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTcgc                                                                                                                                                                                                                                   <  1:655865/236‑1 (MQ=255)
                 agcagGCCCAGCACCATCAGGCCGAGCACCAGCAGCGGGTAAGCGCGCGGGCCCACCGGTTCATAGGAGAATGCAGCCTGATAGGGCCAGGCCATCACGGCCAGGGCGGCACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGTGTCGGGATCGGGCGCGAAGcg                                                                                                                                                                                                                   <  1:262741/235‑2 (MQ=255)
                   cagGCCCAGCCCCATCAGGCCGAGCACCAGCAGCGGGTAAGCGCGCGGGCCCACCGGTTCATAGGAGAATGCAGCCTGATAGGGCCAGGCCATCACGGCCAGGGCGGCACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGcc                                                                                                                                                                                                                <  1:1007424/236‑1 (MQ=255)
                    agGCCCAGCCCCATCAGGCCGAGCACCAGCAGCGGGTAAGCGCGCGGGCCCACCGGTTCATAGGAGAATGCAGCCTGATAGGGCCAGGCCATCACGGCCAGGGCGGCACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCg                                                                                                                                                                                                               >  1:932387/1‑236 (MQ=255)
                                         aGCACCAGCAGCGGGTAAGCGCGCGGGCCCACCGGTTCATAGGAGAATGCAGCCTGATAGGGCCAGGCCATCACGGCCAGGGCGGCACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGCGCGGGCGCGaa                                                                                                                                                                                                                      >  1:110942/1‑208 (MQ=255)
                                         aGCACCAGCAGCGGGTAAGCGCGCGGGCCCACCGGTTCATAGGAGAATGCAGCCTGATAGGGCCAGGCCATCACGGCCAGGGCGGCACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATc                                                                                                                                                                                           >  1:1087972/1‑235 (MQ=255)
                                           aaCCAGCAGCGGGTAAGCGCGCGGGCCCACCGGTTCATAGGAGAATGCAGCCTGATAGGGCCAGGCCATCACGGCCAGGGCGGCACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGcc                                                                                                                                                                                                    <  2:1169112/223‑1 (MQ=255)
                                                 cagcGGGTAAGCGCGCGGGCCCACCGGTTCATAGGAGAATGCAGCCTGATAGGGCCAGGCCATCACGGCCAGGGCGGCACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGaa                                                                                                                                                                                   >  1:609875/1‑235 (MQ=255)
                                                    cGGGTAAGCGCGCGGGCCCACCGGTTCATAGGAGAATGCAGCCTGATAGGGCCAGGCCATCACGGCCAGGGCGGCACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGc                                                                                                                                                                                               <  1:20573/220‑1 (MQ=255)
                                                           gcgcgcGGGCCCACCGGTTCATAGGAGAATGCAGCCTGATAGGGCCAGGCCATCACGGCCAGGGCGGAACACATCGCAAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGcc                                                                                                                                                                                                    <  1:344580/208‑1 (MQ=255)
                                                              cgcgGGCCCACCGGTTCATAGGAGAATGCAGCCTGATAGGGCCAGGCCATCACGGCCAGGGCGGCACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAAc                                                                                                                                                                                                         <  2:792223/200‑1 (MQ=255)
                                                               gcgGGCCCACCGGTTCATAGGAGAATGCAGCCTGATAGGGCCAGGCCATCACGGCCAGGGCGGCACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGTGTCGGGATCGGGCGCGaa                                                                                                                                                                                                                      <  2:1026424/186‑1 (MQ=255)
                                                                cgGGCCCACCGGTTCATAGGAGAATGCAGCCTGATAGGGCCAGGCCATCACGGCCAGGGCGGCACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGa                                                                                                                                                                                                                       <  2:508334/184‑1 (MQ=255)
                                                                  ggCCCGCCGGGTCATAGGAGAAGGCAGCCTGATAGGGCCAGGCCATCACGGCCAGGGCGGCACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGaa                                                                                                                                                                                                                      <  2:103843/183‑1 (MQ=255)
                                                                                   gagaATGCAGCCTGATAGGGCCAGGCCATCACGGCCAGGGCGGCACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGc                                                                                                                                                                                               <  2:952053/189‑1 (MQ=255)
                                                                                        tGCAGCCTGATAGGGCCAGGCCATCACGGCCAGGGCGGCACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGGGGGAGCGGCCTTGCGTcgc                                                                                                                                                                                                                                   >  2:125082/1‑148 (MQ=255)
                                                                                         gCAGCCTGATAGGGCCAGGCCATCACGGCCAGGGCGGCACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTTAACCTTCCGATGCGATCTGt                                                                                                                                                                                        >  1:88324/1‑190 (MQ=255)
                                                                                         gCAGCCTGATAGGGCCAGGCCATCACGGCCAGGGCGGCACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTaa                                                                                                                                                                                      >  1:1143607/1‑191 (MQ=255)
                                                                                         gCAGCCTGATAGGGCCAGGCCATCACGGCCAGGGCGGCACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGTCCGCTTCGCcccc                                                                                                                                              >  2:975942/1‑232 (MQ=255)
                                                                                         gCAGCCTGATAGGGCCAGGCCATCACGGCCAGGGCGGCACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGcc                                                                                                                                                         >  1:696020/1‑221 (MQ=255)
                                                                                         gCAGCCTGATAGGGCCAGGCCATCACGGCCAGGGCGGCACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATc                                                                                                                                          >  1:648282/1‑236 (MQ=255)
                                                                                          cggCCTGATAGGGCCAGGCCATCACGGCCAGGGCGGCACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGa                                                                                                                                                                                    <  1:51659/191‑1 (MQ=255)
                                                                                           aGCCTGATAGGGCCAGGCCATCACGGCCAGGGCGGCACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTc                                                                                                                                                    >  1:413221/1‑224 (MQ=255)
                                                                                                 aTAGGGCCAGGCCATCACGGCCAGGGCGGCACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATCCCGAcaca                                                                                                                                  <  1:906320/236‑1 (MQ=255)
                                                                                                       gagacagaTCACGGCCAGGGCGGCACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATCCCGACACAAGGc                                                                                                                              <  1:699477/227‑1 (MQ=255)
                                                                                                          ggccaTCACGGCCAGGGCGGCACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGa                                                                                                                                                                                                  >  2:958744/1‑163 (MQ=255)
                                                                                                          ggccaTCACGGCCAGGGCGGCACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATCCCGACACAAGGCCGCTc                                                                                                                         >  1:1016343/1‑236 (MQ=255)
                                                                                                            ccaTCACGGCCAGGGCGGCACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGc                                                                                                                                                                                               >  2:1115244/1‑164 (MQ=255)
                                                                                                              aTCACGGCCAGGGCGGCACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGAt                                                                                                                                           >  1:1220950/1‑214 (MQ=255)
                                                                                                              aTCACGGCCAGGGCGGCACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATCCCGACACAAGGCCGCTCCca                                                                                                                      >  1:446159/1‑235 (MQ=255)
                                                                                                                  cGGCCAGGGCGGCACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATg                                                                                                                                                                                                >  2:247357/1‑157 (MQ=255)
                                                                                                                  cGGCCAGGGCGGCACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATg                                                                                                                                                                                                <  1:247357/157‑1 (MQ=255)
                                                                                                                   ggCCAGGGCGGCACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATCCCGACACAAGGc                                                                                                                              >  2:711343/1‑222 (MQ=255)
                                                                                                                     gcAGGGGGGCACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATCCCGACACAAGGcc                                                                                                                             <  2:446156/220‑1 (MQ=255)
                                                                                                                      cAGGGCGGCACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGACTCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGcc                                                                                                                                                                                                    <  2:1132292/149‑1 (MQ=255)
                                                                                                                            ggcACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGc                                                                                                                                                                                 >  2:699474/1‑162 (MQ=255)
                                                                                                                                  cgtCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAAc                                                                                                                                                                                                         <  2:66246/130‑1 (MQ=255)
                                                                                                                                      gCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTcgc                                                                                                                                                                                                                                   <  1:418758/102‑1 (MQ=255)
                                                                                                                                      gCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTcgc                                                                                                                                                                                                                                   >  2:418755/1‑102 (MQ=255)
                                                                                                                                      gCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCGCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTGGCGGTCGGGCGCGAAGCTGCCGCTACATCTGc                                                                                                                                                                                                     >  1:543198/1‑132 (MQ=255)
                                                                                                                                       ccAGCAGGGCCAAGGCGAAAAGGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAACCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGaa                                                                                                                                                                                   <  2:1218882/149‑1 (MQ=255)
                                                                                                                                        cagcagGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGGGCCAGGGGCCGCCTCGCGCCCGAGccc                                                                                                                                        >  1:363764/1‑191 (MQ=255)
                                                                                                                                           cagGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATCCCGACACAAGGCCGCTCCCACAGGAGTGGTGGTGAGTCCg                                                                                                  >  1:808991/1‑226 (MQ=255)
                                                                                                                                             gggCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGc                                                                                                                                                                                               <  1:208216/131‑1 (MQ=255)
                                                                                                                                                ccAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGag                                                                                                                                                                                                                                >  2:203944/1‑94 (MQ=255)
                                                                                                                                                   aGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATCCCGACACAAGGCCGCTCCCACAGGAGTGGTGGTGAGTCCGa                                                                                                 <  1:1015616/219‑1 (MQ=255)
                                                                                                                                                       gAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATCCCGACACAAGGCCGCTCCCACAGGAGTGGTGGTGAGTCCGACCgggt                                                                                           >  1:854179/1‑219 (MQ=255)
                                                                                                                                                                 ggAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATCCCGACACAAGGCCGCTCCCACAGGAGTGGTGGTGAGTCCGACAGGCTTACTGGATCAGGCCGAATTCCtt                                                                    <  2:955496/233‑1 (MQ=255)
                                                                                                                                                                      gATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATCCCGACACAAGGCCGCTCCCACAGGa                                                                                                                 >  1:1040918/1‑184 (MQ=255)
                                                                                                                                                                         cATCGGGGAATCCTCGTTGGGATTGCTCAAGTAAAATGCAAACCCTGTGGGAGCGGCATTGCGTCGTGATCGGGCGCGATGCTGCCGCTAAACCTGCCGATGCGATCTGt                                                                                                                                                                                        <  1:216154/110‑1 (MQ=255)
                                                                                                                                                                           ttGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTACGTCGGGATCTGGCGCGAAGCTGCCGGTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATCCCGACACAAGGCCGCTc                                                                                                                         >  2:654919/1‑171 (MQ=255)
                                                                                                                                                                                 aaTCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGcc                                                                                                                                                                                                    >  2:103007/1‑90 (MQ=255)
                                                                                                                                                                                   tCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATCCCGACACAAGGCCGCTCCCACAGGAGTGGTGGTGAGTCCGACAGGCTTACTGGAGCAGGCCGAAGTCCt                                                                     >  1:114507/1‑215 (MQ=255)
                                                                                                                                                                                     cTCGTGGGGATGGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAACCCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATCCCGACACAAGGc                                                                                                                              <  1:197145/156‑1 (MQ=255)
                                                                                                                                                                                      tCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTg                                                                                                                                                                                                                  <  1:236799/71‑1 (MQ=255)
                                                                                                                                                                                      tCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCCTCGTATCGGCTGTGCCa                                                                                                                                                               >  2:73877/1‑122 (MQ=255)
                                                                                                                                                                                      tCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATCCCGACACAAGGCCGCTCCCACAGGAGTGGTGGTGAGTCCGACAGGCTTACTGGAt                                                                                   >  1:278171/1‑198 (MQ=255)
                                                                                                                                                                                      gcGTTGGGATTGCTCAAGTGAAATGCAAATCATGTGGGAGCGGCCTTGCGTCGCAATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATCCCGCCACAAGGCCGCACCCACAGGAGTGGTGGTGAGTCCGACAGGCTTACTGGATCAGGCCGAATTCCTTGGCCAGCGCCTTGTAGTCAgg                                               >  2:758372/2‑233 (MQ=255)
                                                                                                                                                                                       cGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGc                                                                                                                                                                                               <  2:1252976/89‑1 (MQ=255)
                                                                                                                                                                                         ttGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATCCCGACACAAGGCCGCTc                                                                                                                         <  2:484756/157‑1 (MQ=255)
                                                                                                                                                                                           gggATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATCCCGACACAAGGCCGCTCCCACAGGAGTGGTGGTGAGTCCGACAGGCTTACTGGATCAGGCCGAATTCCTTGGCCAGCGCCTTTTAGTCAGCCAc                                            >  1:898897/1‑232 (MQ=255)
                                                                                                                                                                                                   tCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCACTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATCCCGACACAAGGCCGCTCCCACAGGAGTGGTGGTGAGTCCGACAGGCTTACTGGATCAGGCCGAATTCCTTGGCCAGCGCCTTGTAGTCAGCCACTTGCTTCTTca                                 >  1:525151/1‑235 (MQ=255)
                                                                                                                                                                                                    cAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATCCCGACACAAGGCCGCTCCCACAGGAGTGGTGGTGAGTCCGAc                                                                                                >  1:534775/1‑171 (MQ=255)
                                                                                                                                                                                                               cAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATCCCGACACAAGGCCGCTCCCACAGGAGTGGTGGTGAGTCCGACAGGCTTACTGGATCAGGCCGAATTCCTTGGCCAGCGCCTTGTAGTCAGCCACTTGCTTCTTCACATAGCCGTCCAg                    >  1:595055/1‑236 (MQ=255)
                                                                                                                                                                                                               cAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGACGATGCGATCTGTACGAAGCATAGTATCGGCTGTGCCAGGGGCCGCTTCGCGCACGAACCCGacaac                                                                                                                                  >  1:70176/1‑124 (MQ=255)
                                                                                                                                                                                                                 aaTCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATCCCGACACAAGGCCGCTCCCACAGGAGTGGTGGTGAGTCCGACAGGCTTACTGGATCAGGc                                                                              <  2:1016340/176‑1 (MQ=255)
                                                                                                                                                                                                                    ccTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGATGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGCGCCAGGGGCCGCTTCGCGcc                                                                                                                                               >  2:119052/1‑108 (MQ=255)
                                                                                                                                                                                                                     cTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATCCCGACACAAGGCCGCTCCCACAGGAGTGGTGGTGAGTCCGACAGGCTTACTGGATCAGGCCGAATTCCTTGGCCAGCGCCTTGTAGTCAGCCACTTg                                         <  2:1087969/209‑1 (MQ=255)
                                                                                                                                                                                                                          ggAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAgg                                                                                                                                                             >  2:445291/1‑88 (MQ=255)
                                                                                                                                                                                                                          ggAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATCCCGACACAAGGCCGCTCCCACAGGAGTGGTGGTGAGTCCGACAGGCTTACTGGATCAGGCCGAATTCCTTGGCCAGCGCCTTGTAGTCAGCCACTTGCTTCTTCACATAGCCGTCCAGCTCTTCGcc           >  1:1259627/1‑234 (MQ=255)
                                                                                                                                                                                                                          ggAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATCCCGACACAAGGCCGCTCCCACAGGAGTGGTGGTGAGTCCGACAGGCTTACTGGATCAGGCCGAATTCCTTGGCCAGCGCCTTGTAGTCAGCCACTTGCTTCTTCACATAGCCGTCCAGCTCTTCGCCg          >  1:1248964/1‑235 (MQ=255)
                                                                                                                                                                                                                             gCGGCCTTGTGTCGGGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATCCCGACACAAGGCCGCTCCCACAGGTgtggt                                                                                                            >  1:297920/1‑134 (MQ=255)
                                                                                                                                                                                                                                 ccTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGcc                                                                                                                                               >  1:320235/1‑95 (MQ=255)
                                                                                                                                                                                                                                 ccTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATCCCGACACAAGGCCGCTCCca                                                                                                                      >  2:46825/1‑120 (MQ=255)
                                                                                                                                                                                                                                 ccTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATCCCGACACAAGGCCGCTCCCACAGGAGTGGTGGTGAGTCCGACAGGCTTACTGGATCAGGCCGAATTCCTTGGCCAGCGCCTTGTAGTCAGCCACTTGCTTCTTCACATa                             >  2:750918/1‑209 (MQ=255)
                                                                                                                                                                                                                                 ccTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATCCCGACACAAGGCCGCTCCCACAGGAGTGGTGGTGAGTCCGACAGGCTTACTGGATCAGGCCGAATTCCTTGGCCAGCGCCTTGTAGTCAGCCACTTGCTTCTTCACATAGCCGTCCAGCTCTTCGCCGGTCATGGc  >  1:82733/1‑236 (MQ=255)
                                                                                                                                                                                                                                  cTTGTGTCGGGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATCCCGACACAAGGCCGCTCCCACAGGAgtg                                                                                                              >  1:775768/1‑127 (MQ=255)
                                                                                                                                                                                                                                  cTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATCCCGACACAAGGCCGCTCCCACAGGAGTGGTGGTGAGTCCGACAGGCTTACTGGATCAGGCCGAATTCCTTGGCCAGCGCCTTGTAGTCAGCCACTTGcttct                                    <  2:520525/201‑1 (MQ=255)
                                                                                                                                                                                                                                      cGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATCCCGACACAAGGCCGCTCCCACAGGAGTGGTGGTGAGTCCGAc                                                                                                <  2:702111/137‑1 (MQ=255)
                                                                                                                                                                                                                                       gTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCTATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATCCCGACACAAGGCCGCTCCCACAGGAGTGGTGGTGAGTCCGACAGGCTTACTGGATCAGGCCGAATTCCTTGGc                                                                 <  2:738279/167‑1 (MQ=255)
                                                                                                                                                                                                                                         cgcgATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATCCCGACACAAGGCCGCTCCCACAGGAGTGGTGGTGAGTCCGACAGGCTTACTGGATCAGGCCGAATTCCTTGGCCAGCGCCTTGTAGTCAGCCACTTGCTTCTTCACATAGcc                          <  1:542000/204‑1 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                 
GGCGGAACACCAGGTACAGCAGGCCCAGCCCCATCAGGCCGAGCACCAGCAGCGGGTAAGCGCGCGGGCCCACCGGTTCATAGGAGAATGCAGCCTGATAGGGCCAGGCCATCACGGCCAGGGCGGCACACATCGCCAGCAGGGCCAAGGCGAAAATGCGTTGAAGGATCATTGGGGAATCCTCGTTGGGATTGCTCAAGTGAAATGCAAATCCTGTGGGAGCGGCCTTGCGTCGCGATCGGGCGCGAAGCTGCCGCTAAACCTGCCGATGCGATCTGTACGAAGCATCGTATCGGCTGTGCCAGGGGCCGCTTCGCGCCCGATCCCGACACAAGGCCGCTCCCACAGGAGTGGTGGTGAGTCCGACAGGCTTACTGGATCAGGCCGAATTCCTTGGCCAGCGCCTTGTAGTCAGCCACTTGCTTCTTCACATAGCCGTCCAGCTCTTCGCCGGTCATGGC  >  AE015451/1617547‑1618007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑