Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	AE015451	2,087,670	C→T	14.8%	intergenic (+483/+435)	PP_1865 → / ← aroH	transposase/Phospho‑2‑dehydro‑3‑deoxyheptonate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	2,087,670	0	C	T	14.8%	139.7 / 16.5	54	intergenic (+483/+435)	PP_1865/aroH	transposase/Phospho‑2‑dehydro‑3‑deoxyheptonate aldolase
Reads supporting (aligned to +/- strand):  ref base C (26/20);  new base T (4/4);  total (30/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

GCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGTCATCTGGATAAGGATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGTTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCCGGAAGGGCGGAAAGGTGAATAGGCGCCT  >  AE015451/2087439‑2087883                                                                                                                                                                                                                                        |                                                                                                                                                                                                                        gcTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGTCATCTGGATAAGGATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAgc                                                                                                                                                                                                                         >  2:952887/1‑232 (MQ=255)                                        aTCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGTCATCTGGATAAGGATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGGTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGTGGAGGGAGGTGACGGGcct                                                                                                                                                                                           <  1:643509/223‑3 (MQ=255)                                            gccggcACCGCCGATGCATTCGGCGAGGTTCTGCCCGGTCATCTGGATAAGGATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTACTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAgcg                                                                                                                                                                           <  1:562512/235‑1 (MQ=255)                                                 cACCGCCGATGCATTCGGCGAGGTTCTGCCCGGTCATCTGGATAAGGATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCggagggag                                                                                                                                                                                                      <  1:292286/203‑1 (MQ=255)                                                                         tCTGCCCGGTCATCTGGATAAGGATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGAt                                                                                                                                                                                       >  2:303665/1‑194 (MQ=255)                                                                            gCCCGGTCATCTGGATAAGGATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTg‑gg                                                                                                                                                                     >  2:1207271/1‑208 (MQ=255)                                                                                   cATCTGGATAAGGATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCgg                                                                                                                                  <  1:1179186/235‑1 (MQ=255)                                                                                     tCTGGATAAGGATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATgg                                                                                                                                                                                         >  1:684886/1‑180 (MQ=255)                                                                                     tCTGGATAAGGATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATgg                                                                                                                                                                                         <  2:684883/180‑1 (MQ=255)                                                                                     tCTGGATAAGGATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATg                                                                                                                                                                                          >  1:1053373/1‑179 (MQ=255)                                                                                     tCTGGATAAGGATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGGCTGCAgcg                                                                                                                                              >  1:849487/1‑221 (MQ=255)                                                                                        ggATAAGGATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAgcg                                                                                                                                                                           <  1:28787/191‑1 (MQ=255)                                                                                               gATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGa                                                                                                                     >  1:541889/1‑236 (MQ=255)                                                                                                  atCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAg                                                                                                                    <  2:541886/234‑1 (MQ=255)                                                                                                    cGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGTGCTTGCGGAGGGAGGTGa                                                                                                                                                                                                  >  1:132985/1‑156 (MQ=255)                                                                                                       cGGCAGGGCTGCCCTCGGCCTGGTGTACCGGGGAGAACGCCGTTGCTCTGGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTg                                                                                                                                                                                                               <  2:1147212/140‑1 (MQ=255)                                                                                                            tGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCt                                                                                                                                                    >  2:184781/1‑192 (MQ=255)                                                                                                                tGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGag                                                                                                    <  1:184781/236‑1 (MQ=255)                                                                                                                  cccTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTCCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGGAGGCATTGCGGAGGGAGATGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGcgcgtgctccc                                                                                                                                     >  1:821254/1‑196 (MQ=14)                                                                                                                  cccTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTCCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGGAGGCATTGCGGAGGGAGATGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGcgcgtgctccc                                                                                                                                     <  2:821251/203‑8 (MQ=14)                                                                                                                    atCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTATTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATgg                                                                                                                                                                                         <  1:344861/148‑1 (MQ=255)                                                                                                                     tCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGccc                                                                                                                                                                  >  1:1044378/1‑170 (MQ=255)                                                                                                                       ggCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGa                                                                                                                     >  1:1188950/1‑212 (MQ=255)                                                                                                                          cTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTCCAGGCGACGCAGATTGCGACTTCAGGAGGCCGGGTGGAGGCATTGCGGAGGGAGATGACGGGCATGGATGccc                                                                                                                                                                                   >  1:989333/1‑149 (MQ=17)                                                                                                                                tACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTg                                                                                                                                                                        >  2:927381/1‑154 (MQ=255)                                                                                                                                     ggAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGGGAGGGGACCCCGGAGCGTAGCGTAggg                                                                                        >  2:888717/1‑227 (MQ=255)                                                                                                                                        agaaCGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGccc                                                                                                                                                                  >  2:408549/1‑151 (MQ=255)                                                                                                                                          aaCGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAgcg                                                                                                                                                                                                                        >  1:1022812/1‑96 (MQ=255)                                                                                                                                          aaCGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATgg                                                                                                                                                                                         >  1:132198/1‑127 (MQ=255)                                                                                                                                          aaCGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAgc                                                                                                                                                                            <  1:1018303/140‑1 (MQ=255)                                                                                                                                          aaCGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAgc                                                                                                                                                                            >  2:1018300/1‑140 (MQ=255)                                                                                                                                           aCGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGcccaa                                                                                                                                                                >  2:942536/1‑148 (MQ=255)                                                                                                                                              ccGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTCCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGGAGGCATTGCgg                                                                                                                                                                                                            <  1:788218/104‑1 (MQ=17)                                                                                                                                               cGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGagcgt                                                                                                 >  2:700757/1‑208 (MQ=40)                                                                                                                                               cGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTCGCGTAGGGGCCGGATGATGGGAGcc                                                                       >  2:974440/1‑234 (MQ=255)                                                                                                                                               cGTTGCTCTTGCTCTTGCGCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGcgcg                                                                                                                                            <  1:976436/165‑1 (MQ=255)                                                                                                                                                gTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGAc                                                                    <  1:555439/236‑1 (MQ=40)                                                                                                                                                 ttgctcttgctcttgctcTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAg                                                                                                                                                                             <  1:632401/132‑1 (MQ=255)                                                                                                                                                  tgctcttgctcttgctctTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGTCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGACTGCAGCGCGGTCCTccc                                                                                                                                    >  1:97878/1‑170 (MQ=255)                                                                                                                                                   gctcttgctcttgctcttGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATgggg                                                                          >  2:555436/1‑226 (MQ=39)                                                                                                                                                      ccgaattcgcttctcgctTTTGATCTTGCTTCTAAGCGCGCGGTAGTCCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGtttt                                                              >  1:882625/12‑236 (MQ=11)                                                                                                                                                       cgaattcgcttctcgctTTTGATCTTGCTTCTAAGCGCGCGGTAGTCCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGa                                                                          <  2:882622/213‑1 (MQ=11)                                                                                                                                                                  cttgctTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGTGCGTGCggagc                                                                                                                                                                                                         >  2:800481/1‑86 (MQ=255)                                                                                                                                                                            cTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑g                                                                                                                                                              >  1:1277325/1‑118 (MQ=32)                                                                                                                                                                            cTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑g                                                                                                                                                              <  2:1277322/118‑1 (MQ=32)                                                                                                                                                                                  cTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAggg                                                                                                              >  1:614367/1‑160 (MQ=31)                                                                                                                                                                                    tCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGagcgtagc                                                                                              <  1:303666/174‑1 (MQ=31)                                                                                                                                                                                                            aCGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGCTTTGGTTACt                                                       >  1:865315/1‑189 (MQ=14)                                                                                                                                                                                                              gCAGATTGCGACTTCAGGAGGCCGAGTGGAGGCATTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCTGGATGATGGGa                                                                          <  1:711193/168‑1 (MQ=11)                                                                                                                                                                                                              gCAGATTGCGACTTCAGGAGGCCGAGTGGAGGCATTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCTGGATGATGGGa                                                                          >  2:711190/1‑168 (MQ=11)                                                                                                                                                                                                               cAGATTGCGACTTCAGGAGGCCGAGTGGAGGCATTGCGGAGGGAGATGACGGGCATCCATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGTTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCCGGAAGGGCGGAAAGGTGa            >  1:574159/1‑229 (MQ=255)                                                                                                                                                                                                                aGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGcc                                                                       <  2:1153418/169‑1 (MQ=32)                                                                                                                                                                                                                aGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGcc                                                                       >  1:1153421/1‑169 (MQ=31)                                                                                                                                                                                                                  aTTGCGCCTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGTTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCCGGAAGGGCGGAAAGGTGa            <  1:960227/226‑1 (MQ=17)                                                                                                                                                                                                                     gCGACTTCAGGAGGCCGAGTGGAGGCATTGCGGAGGGAGATGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGtttt                                                              <  1:1147215/173‑1 (MQ=255)                                                                                                                                                                                                                            cAGGAGGCCGGGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGTTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCCGGAAGGGCGGAAAGGTGAATAGGCGCCt  <  1:952890/226‑1 (MQ=18)                                                                                                                                                                                                                                       gcgcAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGTTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCCGGa                           >  2:768147/1‑190 (MQ=33)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                        GCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGTCATCTGGATAAGGATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGTTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCCGGAAGGGCGGAAAGGTGAATAGGCGCCT  >  AE015451/2087439‑2087883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑