| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 278,742:1 | +A | 100% | intergenic (‑237/+77) | PP_0224 ← / ← sctC | Monooxygenase, DszC family/sulfur compound ABC transporter ‑ ATP‑binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 278,742 | 1 | . | A | 95.4% | 159.1 / 0.4 | 43 | intergenic (‑237/+77) | PP_0224/sctC | Monooxygenase, DszC family/sulfur compound ABC transporter ‑ ATP‑binding subunit |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base A (36/5); total (37/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTCAATGCCCTGGCGTTACGCACTGGGGTGATTGTGATCCTGACCATTCCTGACCTCACAAAATAGTTATGTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGT‑A‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCTGCAAACAGGGCCTTGGCCTCGCCTTGCTCCACGATCACGCCTTTGTCGAAGAAAATCACCCGGTTCGCCACGTCCCGG > AE015451/278527‑278963 | cTCAATGCCCTGGCGTTACGCACTGGGGTGATTGTGATCCTGACCATTCCTGACCTCACAAAATAGTTATGTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGT‑a‑a < 2:313884/218‑1 (MQ=255) aaTGCCCTGGCGTTACGCACTGGGGTGATTGTGATCCTGACCATTCCTGACCTCACAAAATAGTTATGTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGT‑a‑aac < 2:786983/217‑3 (MQ=255) cccTGGCGTTACGCACTGGGGTGATTGTGATCCTGACCATTCCTGACCTCACAAAATAGTTATGTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCaa < 1:788870/236‑1 (MQ=255) atcTCACAAAATAGTTATGTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGT‑a‑aac < 2:127147/166‑3 (MQ=255) aaaTAGTTATGTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCCAGATCTGTGCACTGTAGGAGCg > 1:25712/1‑204 (MQ=255) tATGTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCTAAAGGGCCGGAACAgg > 1:380220/1‑225 (MQ=255) tGTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACCGGTTTACTCGGAGGc > 1:528223/1‑236 (MQ=255) cTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGa > 2:767430/1‑213 (MQ=255) aGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCAcg > 1:314369/1‑176 (MQ=255) aGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTccc > 1:725613/1‑236 (MQ=255) gtccggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑ACCGGCTCCTACACACATGCCTCAAGATCTGTGCACTGTa > 2:368852/1‑155 (MQ=255) cggtggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCcgcg > 2:424196/1‑234 (MQ=255) gtgtatataCCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGc > 2:782436/4‑189 (MQ=255) gtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTa > 2:557148/1‑226 (MQ=255) aTGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTcttcctt > 2:613564/1‑223 (MQ=255) gATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGGAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGa > 2:142264/1‑187 (MQ=255) gATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGa > 2:280903/1‑187 (MQ=255) gATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGa < 1:280905/187‑1 (MQ=255) gATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCgg > 2:777567/1‑224 (MQ=255) gATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCTGCAAACAg > 1:742232/1‑236 (MQ=255) gATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCTGCAAACAg > 1:428480/1‑236 (MQ=255) gATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGG‑TTACCcg > 1:46305/1‑141 (MQ=255) aaaaTTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCTGCAAACAGGGCCTTg > 1:702964/1‑236 (MQ=255) aaaaTTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGAGGGTAA‑AACCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAgga > 2:267419/1‑153 (MQ=255) aaaatTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCTGCAAACAGGGCCTTGGcc > 2:433488/4‑213 (MQ=255) atatTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCTGCAAACAGGGCCTTGGCCTCGCCTTGCTCCACGATCACg < 2:702962/233‑1 (MQ=255) tttATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTAAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCTGCAAACAGGGCCTTGGCCTCGCCTTGCTCCACGATCACGCCTTTg > 1:117446/1‑236 (MQ=255) ggATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCTGCACACAGGGCCt > 1:44672/1‑192 (MQ=255) gATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCg > 1:279219/1‑118 (MQ=255) gATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGCGGa > 2:173706/1‑137 (MQ=255) gATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCTGCAAACAGGGCCTTGGCCTCGCCTTGCTCCACGAt > 1:705747/1‑213 (MQ=255) gATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCTGCAAACAGGGCCTTGGCCTCGCCTTGCTCCACGATCACGc > 1:521606/1‑218 (MQ=255) gATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCGCTTCCTTCGGCGCTGCAAACAGGGCCTTGGCCTCGCCtt > 1:659286/1‑203 (MQ=255) gATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGT‑AGACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCTGCAAACAGGGCCTTGGCCTCGCCTTGCTCCACGATCACGCCTTTGTCGAAGAAAATCa > 1:481078/1‑236 (MQ=255) ggTGCCGGGCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCTGCAAACAGGGCCTTGGCCTCGCCTTGCTCCACGATCACGCCTTTGTTgac > 1:442025/1‑214 (MQ=255) ccGGGCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAgg > 1:7507/1‑119 (MQ=255) ccGGGCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCTGCAAACAGGGCCTTGGCCTCGCCTTGCTCCACGATCACGCCTTTGTCGAAGAAAATCACCCGGTTCGCCa > 2:577127/1‑231 (MQ=255) ccGGGCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCTGCAAACAGGGCCTTGGCCTCGCCTTGCTCCACGATCACGCCTTTGTCGAAGAAAATCACCCGGTTCGCCACGt > 2:672514/1‑234 (MQ=255) ccGGGCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCTGCAAACAGGGCCTTGGCCTCGCCTTGCTCCACGATCACGCCTTTGTCGAAGAAAATCACCCGGTTCGCCACGTcc > 1:560303/1‑236 (MQ=255) ccGGGCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGAAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCTGCaa > 1:678487/1‑165 (MQ=255) ccGGGCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGAAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCTGCaa < 2:678485/165‑1 (MQ=255) gggCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCTGCAAACAGGGCCTTGGCCTCGCCTTGCTc > 2:600980/1‑188 (MQ=255) gggCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCTGCAAACAGGGCCTTGGCCTCGCCTTGCTCCACGATCACGCCTTTGTCGAAGAAAATCACCCGGTTCGCCACGTCCCg > 1:776821/1‑236 (MQ=255) ggCTCTTCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCTGCAAACAGGGCCTTGGCCTCGCCTTGCTCCACGATCACGCCTTTGTCGAAGAAAATCACCCGGTTCGCCACGTCCCgg < 1:424198/236‑1 (MQ=255) gccctttCGCGGGTAA‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGGTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGGACGCTCTTCCTTCGGCGCTGCAAACAGGGCCTTGGCCTCGCCTTGCTCCACGATCCCGCCTTTGt > 1:178213/6‑204 (MQ=255) tctTCGCGGGT‑AGACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCTGCAAACAGGGCCTTGGCCTCGCCTTGCTCCACGATCACGCCTTTGTCGAAGAAAATCACCCGGTTCGCCACGTCCCgg < 2:481076/233‑1 (MQ=255) | CTCAATGCCCTGGCGTTACGCACTGGGGTGATTGTGATCCTGACCATTCCTGACCTCACAAAATAGTTATGTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGT‑A‑ACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCTGCAAACAGGGCCTTGGCCTCGCCTTGCTCCACGATCACGCCTTTGTCGAAGAAAATCACCCGGTTCGCCACGTCCCGG > AE015451/278527‑278963 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |