New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 2412875	32 (0.730)	3 (0.070)	3/322	NT	8.8%	intergenic (+139/‑282)	rlmM/PP_2114	Ribosomal RNA large subunit methyltransferase M/transposase
	?	AE015451	= 4908026	32 (0.780)					intergenic (‑2/‑280)	PP_4317/PP_4318	conserved protein of unknown function/transposase

GGTGTCGATTGCCTGCAAGCAGCTGTACCACGACCGCGAGGAAGTGACGTGCCACCTGCGGCGGCTCGATTTGAAGCCACGCTGAGGCCAGCACGTCGTTCTGCTTCTGCGTCTGCGTCTGCTTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACCCGCCGTAAGGGCCATCCCTTTCAAGGTCTTCGGTAGAATTCGCTGAAATCCAGTCAGGAAGACCCGAGCGATG  >  AE015451/2412652‑2413108                                                                                                                                                                                                                                |                                                                                                                                                                                                                                          ggTGTCGATTGCCTGCAAGCAGCTGTACCACGACCGCGAGGAAGTGACGTGCCACCTGCGGCGGCTCGATTTGAAGCCACGCTGAGGCCAGCACGTCGTTCTGCTTCTGCGTCTGCGTCTGCTTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTc                                                                                                                                                                                                                                <  1:554498/235‑1 (MQ=255)        aTTGCCTGCAAGCAGCTGTACCACGACCGCGAGGAAGTGACGTGCCACCTGCGGCGGCTCGATTTGAAGCCACGCTGAGGCCAGCAAGTCGTTCTGCTTCTGCGTCTGCGTCTGCTTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACTCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCt                                                                                                                                                                                                                         <  1:428117/235‑1 (MQ=255)                  agcagcTGTACCACGACCGCGAGGAAGTGACGTGCCACCTGCGGCGGCTCGATTTGAAGCCACGCTGAGGCCAGCACGTCGTTCTGCTTCTGCGTCTGCGTCTGCTTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTg                                                                                                                                                                                                                        <  2:578050/226‑1 (MQ=255)                            ccACGACCGCGAGGAAGTGACGTGCCACCTGCGGCGGCTCGATTTGAAGCCACGCTGAGGCCAGCACGTCGTTCTGCTTCTGCGTCTGCGTCTGCTTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCt                                                                                                                                                                                                    <  1:317934/236‑1 (MQ=255)                                       aGGAAGTGACGTGCCACCTGCGGCGGCTCGATTTGAAGCCACGCTGAGGCCAGCACGTCGTTCTGCTTCTGCGTCTGCGTCTGCTTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCat                                                                                                                                                                                                               >  1:345048/1‑214 (MQ=255)                                               aCGTGCCGCCTGCGGCGGCTCGATTTGAAGCCACGCTGAGGCCAGCACGTCGTTCTGCTTCTGCGTCTGCGTCTGCTTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAgcg                                                                                                                                                                                              <  1:166363/223‑1 (MQ=255)                                                 gTGCCCCCTGCGGCGGCTCGATTTGAAGCCACGCTGAGGCCAGCACGTCGTTCTGCTTCTGCGTCTGCGTCTGCTTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAgcgc                                                                                                                                                                                                                          <  2:206611/193‑1 (MQ=255)                                                   gCCACCTGCGGCGGCTCGATTTGAAGCCACGCTGAGGCCAGCACGTCGTTCTGCTTCTGCGTCTGCGTCTGCTTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCaa                                                                                                                                                                             >  1:554928/1‑236 (MQ=255)                                                    ccACCTGCGGCGGCTCGATTTGAAGCCACGCTGAGGCCAGCACGTCGTTCTGCTTCTGCGTCTGCGTCTGCTTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCaa                                                                                                                                                                             <  1:94224/235‑1 (MQ=255)                                                         tGCGGCGGCTCGATTTGAAGCCACGCTGAGGCCAGCACGTCGTTCTGCTTCTGCGTCTGCGTCTGCTTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGa                                                                                                                                                                                                                       <  1:62113/188‑1 (MQ=255)                                                          gcggcgGCTCGATTTGAAGCCACGCTGAGGCCAGCACGTCGTTCTGCTTCTGCGTCTGCGTCTGCTTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCatgggt                                                                                                                                                                                                           >  2:488279/1‑197 (MQ=255)                                                          gcggcgGCTAGAGTTGAAGCCACGCTGAGGCCAGCACGTCGTTCTGCTTCTGCGTCTGCGTCTGCTTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAgg                                                                                                                                                                                                                     <  1:194246/189‑1 (MQ=255)                                                                     aTTTGAAGCCACGCTGAGGCCAGCACGTCGTTCTGCTTCTGCGTCTGCGTCTGCTTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACt                                                                                                                                                                                        <  1:626169/207‑1 (MQ=255)                                                                                cGCTGAGGCCAGCACGTCGTTCTGCTTCTGCGTCTGCGTCTGCTTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGa                                                                                                                                                                                                                       <  1:700515/165‑1 (MQ=255)                                                                                             aCGTCGTTCTGCTTCTGCGTCTGCGTCTGCTTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTa                                                                                                                                                                                          >  1:776281/1‑181 (MQ=255)                                                                                               gtcgtTCTGCTTCTGCGTCTGCGTCTGCTTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCATGCAGCGCGTACTCCCGGGAGCAAGCCCggg                                                                                                                                                                      >  2:420528/1‑198 (MQ=255)                                                                                                      tgcttctgcGTCTGCGTCTGCTTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAAcccc                                                                                                                                                         <  1:56335/205‑1 (MQ=255)                                                                                                      tgctgctgcGTCTGCGTCTGCGTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTGCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCg                                                                                                                                                                   <  2:178746/195‑1 (MQ=255)                                                                                                      ggcttctgcGTCTGCGTCTGCTTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAAcccc                                                                                                                                                         <  1:102225/204‑1 (MQ=255)                                                                                                              cgtctgcgtctgCTTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGt                                                                                                                  >  1:273976/1‑236 (MQ=255)                                                                                                                 ctgcgtctgcTTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCa                                                                                                                                                                              >  2:240822/1‑173 (MQ=255)                                                                                                                             ctgcttTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCg                                                                                                                                     >  1:76542/1‑202 (MQ=255)                                                                                                                                   ttGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCTGGAGCAAGCCCGGTGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCg                                                                                                                                     >  2:308125/1‑196 (MQ=255)                                                                                                                                       tGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATccatgcccgtcacctgccttcgcaaggcctgcgttcggcctcctgaagtcgcgaagatcaagatcaagatcaagatcaagatcaagataaagatcaagatcaagatcaagatc                                                                                                                          >  1:476245‑M1/1‑90 (MQ=255)                                                                                                                                             ttCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACaaa                                                                                   >  1:537906/1‑236 (MQ=255)                                                                                                                                                    cgcgcgATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAgg                                                                                                                                          <  2:245327/174‑1 (MQ=35)                                                                                                                                                                 aGGCGACGCAGATTGCGACTTCAGAAGGCCGAGCGCAGGGCTTGCGAAGGGAGGTGACGGGCATccatgcccgtcacctgcctccgcaaggcctgcgttcggcctcctgaagtcgcgaagatcaagatcaagatcaagatcaagatcaagatcaagatcaagatcaacggcaacggcaacggcaacggcaacggcaacagcaacagcaacaacagcacagtacagttttgccttgt                                                               >  1:686579‑M1/1‑64 (MQ=255)                                                                                                                                                                                    ttCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGGTACTTTTTGTCGCGTTTGa                                                                                       >  1:317250/1‑193 (MQ=255)                                                                                                                                                                                         gaggCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGt                                                                               >  1:663565/1‑196 (MQ=40)                                                                                                                                                                                         gaggCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACCCGCCGTAAGGGCCATCCCTTTCAAGGTCTTCGGTAg                                       >  1:192684/1‑236 (MQ=38)                                                                                                                                                                                         gaagCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTg                                                                              <  1:779559/194‑1 (MQ=38)                                                                                                                                                                                                              gAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATGGGAGCGCAGCGTATTTTGGCTACTTTTTGTCTCGt                                                                                           >  2:339721/1‑163 (MQ=255)                                                                                                                                                                                                              gAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTcg                                                                                              <  1:749602/160‑1 (MQ=40)                                                                                                                                                                                                              gAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTcg                                                                                              >  2:749600/1‑160 (MQ=39)                                                                                                                                                                                                                aGGGAGGTGACGGGCATggatgcccgtcacctacctccgcaaggcctgcgttcggcctcctgaagtcgcgaagatcaagatcaagatcaagatcaagatcaagatcaagatcaagatcaacggcaacggcaacggcaacggcaacggcaacagcaacagcaacaa                                                                                       <  2:603995‑M1/165‑149 (MQ=255)                                                                                                                                                                                                                       tGACGGGCATccatgcccgtcacctgcctccgcaaggcctgcgttcggcctcctgaagtcgcgaagatcaagatcaagatcaagatcaagatcaagatcaagatcaagatcaacggcaacggcaacggcaacggcaacggcaacagcaacagcaacaacagcacagtacagttttgccttgttatttccat                                                      <  2:550704‑M1/191‑182 (MQ=255)                                                                                                                                                                                                                          cGGGCATccatgcccgtcacctgcctccgcaaggcctgcgttcggcctcctgaagtcgcgaagatcaagatcaagatcaagatcaag                                                                                                                                                           >  1:580509‑M1/1‑7 (MQ=255)                                                                                                                                                                                                                          cGGGCATccatgcccgtcacctgcctccgcaaggcctgcgttcggcctcctgaagtcgcgaagatcaagatcaagatcaagatcaag                                                                                                                                                           <  2:580507‑M1/87‑81 (MQ=255)                                                                                                                                                                                                                              cATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACCCGCCGTAAGGGCCATCCCTTTCAAGGTCTTCGGTAGAATTCGCTGAAATCCAGTCAGGAAGACCCGAGCGAtg  >  1:353860/1‑236 (MQ=38)                                                                                                                                                                                                                                tccatgcccgtcacctgcctccgcaaggcctgcgttcggcctcctgaagtc                                                                                                                                                                                         <  1:441392‑M1/51‑51 (MQ=255)                                                                                                                                                                                                                                tccatgcccgtcacctgcctccgcaaggcctgcgttcggcctcctgaagtc                                                                                                                                                                                         >  2:441390‑M1/1‑1 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                                          GGTGTCGATTGCCTGCAAGCAGCTGTACCACGACCGCGAGGAAGTGACGTGCCACCTGCGGCGGCTCGATTTGAAGCCACGCTGAGGCCAGCACGTCGTTCTGCTTCTGCGTCTGCGTCTGCTTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACCCGCCGTAAGGGCCATCCCTTTCAAGGTCTTCGGTAGAATTCGCTGAAATCCAGTCAGGAAGACCCGAGCGATG  >  AE015451/2412652‑2413108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.