Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AE015451 196,006 T→C 9.6% N504N (AAT→AAC)  PP_0168 → putative surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451196,0060TC 9.6% 136.6 / 5.5 52N504N (AAT→AAC) PP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base T (19/28);  new base C (2/3);  total (21/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.64e-01

CAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACTGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACGGTCAGCGTCACCATCGAGAACGCCACGGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCG  >  AE015451/195776‑196238
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                        
cAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGc                                                                                                                                                                                                                                     >  1:755690/1‑236 (MQ=39)
         gTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGcc                                                                                                                                                                                                                            <  1:617434/236‑1 (MQ=37)
           cGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCg                                                                                                                                                                                                                           >  1:483461/1‑235 (MQ=38)
            gttttCCCGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGcc                                                                                                                                                                                                                            <  2:483459/231‑1 (MQ=37)
                tCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACt                                                                                                                                                                                                                     >  1:532236/1‑236 (MQ=40)
                  cAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGATCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGtgacag                                                                                                                                                                                                                    <  1:442630/235‑6 (MQ=37)
                     aCCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACTGTGAc                                                                                                                                                                                                                >  1:282923/1‑236 (MQ=40)
                           gCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACTGTGACCCTg                                                                                                                                                                                                            <  2:755688/234‑1 (MQ=255)
                                 gACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACTGTGACCCTGTCCAAc                                                                                                                                                                                                      <  2:282921/234‑1 (MQ=39)
                                       tACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACTGTGACCCTGTCCAACGGCCaaa                                                                                                                                                                                               >  1:331918/1‑235 (MQ=39)
                                                  cTCGACCGTCAGCGTCACCATCGAGAGCGCCACCGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACTGTGACCCTGTCCAACGGCCAAAc                                                                                                                                                                                              <  1:82500/225‑1 (MQ=25)
                                                    cGACCGTCAGCGTCACCATCGAGAGCGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGCCCCCGGTGACAGTGAccc                                                                                                                                                                                                              >  2:737319/1‑207 (MQ=12)
                                                                  cccATCGAGAGCGCCACCGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACTGTGACCCTGTCCAACGGCCAAACGATTACCGTTGa                                                                                                                                                                                  <  1:268514/220‑1 (MQ=37)
                                                                     aTCGAGAGCGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACTGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAgg                                                                                                                                                                 >  1:733659/1‑235 (MQ=255)
                                                                                   cggcggcAACTTCGAACAGCTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGa                                                                                                                                                                                                                                >  1:472133/2‑158 (MQ=255)
                                                                                     gcggcAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACTGTGACCCTGTCCAAc                                                                                                                                                                                                      <  2:570429/182‑1 (MQ=38)
                                                                                       ggcAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACTGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCgg                                                                                                                                                                             <  2:532234/205‑1 (MQ=40)
                                                                                                    aacTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACTGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCg                                                                                                                                                           <  1:803636/208‑1 (MQ=39)
                                                                                                     gCTGACCCCGCACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGcc                                                                                                                                                                                                                            <  1:14704/144‑1 (MQ=21)
                                                                                                                ccccGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACTGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCgg                                                                                                                                                                             <  2:315540/179‑1 (MQ=25)
                                                                                                                   cGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACTGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAg                                                                                                                                                                                >  1:367839/1‑174 (MQ=39)
                                                                                                                   cGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACTGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCgg                                                                                                                                                                             >  1:685437/1‑177 (MQ=255)
                                                                                                                   cGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACTGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCgg                                                                                                                                                                             <  2:685435/177‑1 (MQ=255)
                                                                                                                   cGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGt                                                                                                                  >  1:286490/1‑236 (MQ=2)
                                                                                                                          ggCTCAGCCCTCTATCAACGACTCGGTCGACGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACCCCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACTGTGACCCTGTCCAAc                                                                                                                                                                                                      <  2:314655/145‑1 (MQ=11)
                                                                                                                                       aTCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACTGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGAc                                                                                                                                              <  2:331916/188‑1 (MQ=39)
                                                                                                                                                  ggTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGcc                                                                                                                                                                                                                            >  2:124329/1‑99 (MQ=37)
                                                                                                                                                  ggTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGcc                                                                                                                                                                                                                            <  1:124331/99‑1 (MQ=37)
                                                                                                                                                     cGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACTGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTac                                                                                                                           <  2:367837/193‑1 (MQ=37)
                                                                                                                                                            accaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACTGTGACCCTGTCCAACGGCCAAACGATTa                                                                                                                                                                                         >  1:429670/1‑124 (MQ=17)
                                                                                                                                                                     aCCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACTGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGtc                                                                                                                                                                          >  1:137956/1‑129 (MQ=255)
                                                                                                                                                                           aCGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACTGTGACCCTGTCCAAc                                                                                                                                                                                                      >  1:784460/1‑96 (MQ=37)
                                                                                                                                                                             ggtgAGCACGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACTGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCa                                                                                                                                      <  2:503028/155‑1 (MQ=255)
                                                                                                                                                                              gCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACTGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAAt                                                                                                                                    >  1:439799/1‑159 (MQ=255)
                                                                                                                                                                              gCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACTGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAAt                                                                                                                                    <  2:439797/159‑1 (MQ=40)
                                                                                                                                                                                  gCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACTGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACGGTCAGCGTCACCATCGAGAACGcc                                                                                    <  2:733657/203‑1 (MQ=255)
                                                                                                                                                                                    ccgaccgTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACTGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCa                                                                                                                                      <  1:108421/145‑1 (MQ=255)
                                                                                                                                                                                          gTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACTGTGACCCTGTCCAACGGc                                                                                                                                                                                                   <  1:672291/84‑1 (MQ=38)
                                                                                                                                                                                                    tcggcgTGATCGCCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACtg                                                                                                                                                                                                                    <  1:151419/56‑1 (MQ=255)
                                                                                                                                                                                                              cACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACTGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACGGTCAGCGTCACCATCGAGAACGCCACGGGCGGTAAttt                                                                      <  2:286488/189‑1 (MQ=255)
                                                                                                                                                                                                                 cTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACTGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACGGTCAGCGTCACCATCGAGAACGcc                                                                                    <  1:271097/172‑1 (MQ=255)
                                                                                                                                                                                                                   acacCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACGGTCAGCGTCACCATCGAGAACGCCACGGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGAc                     <  2:355315/233‑1 (MQ=255)
                                                                                                                                                                                                                       cGTGACCCCGAGCAACCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACGGTCAGCGTCACCATCGAGAACGCCACGGGCGGTAATTTCGAGc                                                                 <  2:260107/185‑1 (MQ=255)
                                                                                                                                                                                                                            cccTGAGCAATCCTGCCCAGACGCCGGTAACTGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGAc                                                                                                             <  2:719717/136‑1 (MQ=255)
                                                                                                                                                                                                                            cccTGAGCAATCCTGCCCAGACGCCGGTAACTGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGAc                                                                                                             >  1:719719/1‑136 (MQ=255)
                                                                                                                                                                                                                              cTGAGCAATCCTGCCCAGACGCCGGTAACTGTGACCCTGt                                                                                                                                                                                                           >  2:215791/1‑40 (MQ=40)
                                                                                                                                                                                                                              cTGAGCAATCCTGCCCAGACGCCGGTAACTGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACGGTCAGCGTCACCATCGAGAACGCCACGGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAAc                        <  1:249284/219‑1 (MQ=255)
                                                                                                                                                                                                                              cTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACGGTCAGCGTCACCATCGAGAACGCCACGGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCgg                                         >  2:773427/1‑202 (MQ=255)
                                                                                                                                                                                                                              cTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACGGTCAGCGTCACCATCGAGAACGCCACGGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCgg                                         <  1:773429/202‑1 (MQ=255)
                                                                                                                                                                                                                                    aaTCCTGCCCAGACGCCGGTAACTGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCgg                                                                                                                                                                             <  1:94057/64‑1 (MQ=255)
                                                                                                                                                                                                                                    aaTCCTGCCCAGACGCCGGTAACTGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACGGTCAGCGTCACCATCGAGAACGCCACGGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACcg  >  1:674946/1‑235 (MQ=255)
                                                                                                                                                                                                                                    aaTCCTGCCCAGACGCCGGTAACTGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACGGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGa                                                                   >  1:732068/1‑170 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                        
CAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACTGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACGGTCAGCGTCACCATCGAGAACGCCACGGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCG  >  AE015451/195776‑196238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑