Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AE015451 5,674,753 G→C 100% intergenic (+115/+25) rhlE‑II → / ← yceI ATP‑dependent RNA helicase/polyisoprene‑binding protein, acidic stress response factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE0154515,674,7530GC100.0% 155.3 / NA 45intergenic (+115/+25)rhlE‑II/yceIATP‑dependent RNA helicase/polyisoprene‑binding protein, acidic stress response factor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (25/20);  total (25/20)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

CGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGC‑GCGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTGACACCGTGCAT  >  AE015451/5674555‑5674972
                                                                                                                                                                                                       |                                                                                                                                                                                                                           
cGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCtt                                                                                                                                                                                         <  1:690594/236‑1 (MQ=255)
    tCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTg                                                                                                                                                                                                            <  1:209916/213‑1 (MQ=255)
              tGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGaca                                                                                                                                                                            <  1:440759/235‑1 (MQ=255)
                  cAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGat                                                                                                                                                                       <  1:100124/236‑1 (MQ=255)
                               cgCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGat                                                                                                                                                                       <  2:501312/222‑1 (MQ=255)
                                                                          cAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCg                                                                                                                                                                                                                       >  2:1313/1‑132 (MQ=255)
                                                                               gCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGCTTTCAGCTGGCAGAACTTACTTCTGCTTCCCACCTTCGACACTGATATCCCGGTCCAGGGTc                                                                                                                                                       >  2:172388/1‑191 (MQ=38)
                                                                                       aaGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGaca                                                                                                                                                                            <  2:705739/162‑1 (MQ=255)
                                                                                            ccGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGCTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGACGt                                                                                                                     >  1:138252/1‑212 (MQ=255)
                                                                                             cGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCt                                                                                                                                  <  1:36007/198‑1 (MQ=255)
                                                                                               aGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGat                                                                                                                                                                       <  1:745303/159‑1 (MQ=255)
                                                                                                                     tCACGACACAAGCCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACGCTGATATCCAGGTCCAGGGTGTGCGAAGTAGCACCCGGGCCTTTGATGCCGAGGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAgcgcggt                                                                           >  1:763321/1‑227 (MQ=255)
                                                                                                                        cGACACACGGCCGCTCCTGCAGAGAATTTCGTGATCTCTAGAGGAGCGGCCGTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGcc                                                                                                                          <  2:249115/179‑1 (MQ=255)
                                                                                                                        cGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGCAGCCAGCGCGCTCGCCAccc                                                                   >  2:13662/1‑234 (MQ=255)
                                                                                                                            acaAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATggg                                                             <  1:435376/236‑1 (MQ=255)
                                                                                                                             caAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGc                                                                                                                                    >  2:406997/1‑164 (MQ=255)
                                                                                                                             caAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGaa                                                                                                                       >  2:276498/1‑177 (MQ=255)
                                                                                                                             caAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGtggtggtgg                                                                                             >  2:322372/1‑203 (MQ=255)
                                                                                                                                      tCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTc                                                           >  1:426633/1‑228 (MQ=255)
                                                                                                                                        cTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGa                                                                                       >  2:389828/1‑198 (MQ=255)
                                                                                                                                        cTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCt                                                    >  2:577668/1‑233 (MQ=255)
                                                                                                                                         tGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGTGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCtt                                                                                                                                 >  2:757211/1‑155 (MQ=255)
                                                                                                                                           cAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCt                                                                           <  2:620508/207‑1 (MQ=255)
                                                                                                                                             gagaATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtg                                                                                                    >  1:626239/1‑180 (MQ=255)
                                                                                                                                                    tCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGTGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCt                                                                           <  2:137473/198‑1 (MQ=255)
                                                                                                                                                           ctctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtc                                                                                                 >  2:544563/1‑168 (MQ=255)
                                                                                                                                                                      cGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAgcgc                                                                              <  1:276500/177‑1 (MQ=255)
                                                                                                                                                                        gCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACCCCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACg                           <  2:135319/226‑1 (MQ=255)
                                                                                                                                                                         ccTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCCGAAATTACTTCTGCTTCACACCTTCGACACTGCTATCCAGGTCCAGGGTCtc                                                                                                                                                     >  2:110315/1‑102 (MQ=38)
                                                                                                                                                                           ttGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGtttt                                                                                                                                                                                                                  >  1:160201/1‑40 (MQ=255)
                                                                                                                                                                           ttGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGa                                          <  2:346713/208‑1 (MQ=255)
                                                                                                                                                                           ttGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGa                                          >  1:346715/1‑208 (MQ=255)
                                                                                                                                                                           ttGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGaa                             >  1:209935/1‑221 (MQ=255)
                                                                                                                                                                           ttGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTgg               >  1:455910/1‑235 (MQ=255)
                                                                                                                                                                           ttGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTg                >  1:73326/1‑234 (MQ=255)
                                                                                                                                                                           ttGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGt              >  1:684746/1‑236 (MQ=255)
                                                                                                                                                                           ttGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGt              >  1:191209/1‑236 (MQ=255)
                                                                                                                                                                           ttGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGGTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCa                                                                      >  2:345894/1‑180 (MQ=255)
                                                                                                                                                                           ttGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGGTTGCAGCTGGCAGAAATTACTTCTGCTTCACACCTGCGCCACTGAAAGCCAGGTCCAGGGTCTGCGAAGTa                                                                                                                                              >  2:738794/1‑108 (MQ=255)
                                                                                                                                                                           gtgtgtgGTGATAGGGCCGCAAAGCGGCCCCCTCGGGTTTCAGCTGGCAGAAAGTACGTCTGCGTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGcc                                                                                                                          <  2:12753/127‑1 (MQ=255)
                                                                                                                                                                            tgtgTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTg             <  1:108127/236‑1 (MQ=255)
                                                                                                                                                                                 cGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTGacac         >  1:89441/1‑235 (MQ=255)
                                                                                                                                                                                  gTGATAGGGCCGCAAAGCGGCCCCCTCGGTGTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTGa            <  1:583146/231‑1 (MQ=255)
                                                                                                                                                                                        gggCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTGACACCGTGCAt  <  1:18003/235‑1 (MQ=255)
                                                                                                                                                                                             gCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCAcc                                                                    <  1:406999/164‑1 (MQ=255)
                                                                                                                                                                                                       |                                                                                                                                                                                                                           
CGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGC‑GCGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTGACACCGTGCAT  >  AE015451/5674555‑5674972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑