Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	AE015451	197,715	C→A	12.7%	T1074N (ACC→AAC)	PP_0168 →	putative surface adhesion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	197,715	0	C	A	12.7%	93.9 / 2.4	38	T1074N (ACC→AAC)	PP_0168	putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base C (13/20);  new base A (2/3);  total (15/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

CACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAG  >  AE015451/197482‑197937                                                                                                                                                                                                                                          |                                                                                                                                                                                                                               cACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCg                                                                                                                                                                                                                              <  1:733035/236‑1 (MQ=37)               tCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCg                                                                                                                                                                                                                              <  2:457757/222‑1 (MQ=40)               tCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCg                                                                                                                                                                                                                              >  1:457759/1‑222 (MQ=40)               tCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGacac                                                                                                                                                                                                                 >  1:588890/1‑235 (MQ=255)               cccTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGacac                                                                                                                                                                                                                 <  1:465880/234‑1 (MQ=14)                                                                     cgaTTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACg                                                                                                                                                                                                                              >  2:541807/3‑168 (MQ=255)                                                                     ccATGACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGt                                                                                                                                                                                                                       <  1:487625/175‑1 (MQ=38)                                                                            cGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCa                                                                                                                                                                                     >  1:358659/1‑202 (MQ=255)                                                                               tGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAggcg                                                                                                                                                                     <  2:588888/215‑1 (MQ=255)                                                                                 aaGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGt                                                                                                                                                                                                                       >  2:487623/1‑163 (MQ=18)                                                                                         aaGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAg                                                                                                                                                                        <  2:358657/202‑1 (MQ=255)                                                                                               cAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCa                                                                                                                                       <  1:652645/229‑1 (MQ=255)                                                                                                  ggCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTTCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATc                                                                                                                                                             <  2:510396/204‑1 (MQ=255)                                                                                                    cAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCg                                                                                                                                                                                                                         >  1:585326/1‑142 (MQ=21)                                                                                                    cAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCg                                                                                                                                                                                                                         <  2:585324/142‑1 (MQ=21)                                                                                                           gATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCgg                                                                                                                     <  1:752610/235‑1 (MQ=11)                                                                                                                                       acaacaACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGa                                                                                                                                                                                                                    <  2:542009/112‑1 (MQ=40)                                                                                                                                                   cGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCACAGACCACGATCACCGACTCGGTCGACACCACTACCGCGACCCTGACCGc                                                                                                                                                                                            >  2:496919/1‑124 (MQ=255)                                                                                                                                                      ccgTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCaa                                                                                                                                                                                    <  2:387201/127‑1 (MQ=255)                                                                                                                                                      ccgTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCaa                                                                                                                                                                                    >  1:387203/3‑129 (MQ=255)                                                                                                                                                        gTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGt                                                                                                                                                                                                                       <  2:307666/92‑1 (MQ=37)                                                                                                                                                                                  aGGCGGTAATTTTGAGCAACTTACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGa                                                                                                                                                                                                                             >  1:202592/1‑60 (MQ=11)                                                                                                                                                                                          aTTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGa                                                                             >  1:462900/1‑196 (MQ=255)                                                                                                                                                                                          aTTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGa                                                                             <  2:462898/196‑1 (MQ=255)                                                                                                                                                                                            tgcGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACCACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCAc                                                                                                                                                           <  2:508060/114‑1 (MQ=255)                                                                                                                                                                                             tCGAGCACCTGACCCCGACCCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGt                                                                                                                                                                                <  2:151287/94‑1 (MQ=255)                                                                                                                                                                                               gAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACGCCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAAc                                                                                                 <  1:302322/171‑1 (MQ=255)                                                                                                                                                                                                        aCCCCGAATCCGACCCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCACGCCAACCGTAACCGAAGGCGGCGTGATCACCTACACCGTGAc                                                                                                                                               >  2:579331/1‑116 (MQ=17)                                                                                                                                                                                                        aCCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTg                                                                                                                                                                                                 >  1:132482/1‑66 (MQ=40)                                                                                                                                                                                                        aCCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTg                                                                                                                                                                                                 <  2:132480/66‑1 (MQ=40)                                                                                                                                                                                                                 ccGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACTGCGACCCTGACCGCCGCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCgg                                                                        <  1:707415/178‑1 (MQ=255)                                                                                                                                                                                                                      gCCGTCTCAGACCACGATCAACGACTCGGTCGACACCACCACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCt                                                                                                                                            <  1:303785/105‑1 (MQ=255)                                                                                                                                                                                                                          gCTCAGACCACGATCACCGACTCGGTCGACACCACCACCGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGcc                                                                                                                               <  2:373259/114‑1 (MQ=255)                                                                                                                                                                                                                             cAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGAc                                                                                                                                                                             <  2:137937/65‑1 (MQ=255)                                                                                                                                                                                                                              aGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAg  >  1:487135/1‑235 (MQ=255)                                                                                                                                                                                                                                aCCACGATCACCGACTCGGTCGacac                                                                                                                                                                                                                 <  2:78556/26‑1 (MQ=255)                                                                                                                                                                                                                                aCCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTCGAAGCgg                                                                        >  2:309771/1‑163 (MQ=255)                                                                                                                                                                                                                                       tCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTc                                              >  2:668034/1‑182 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                               CACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAG  >  AE015451/197482‑197937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑