| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 206,332 | C→T | 9.2% | T3946T (ACC→ACT) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 206,332 | 0 | C | T | 9.2% | 185.8 / 6.7 | 63 | T3946T (ACC→ACT) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base C (29/28); new base T (2/4); total (31/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
CCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGTACCGTGGGTACTGCTACCGTGACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTCGTGATGTCGATCGCAACCGTT > AE015451/206131‑206524 | ccACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGc < 1:433553/236‑1 (MQ=255) tGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGc > 1:130420/1‑230 (MQ=255) cgcAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGt > 2:313943/1‑224 (MQ=255) gcAAGGCGATGACGTCTACACCGACGCTGGCCAGATCAGCCTGGGCATCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGc < 1:163635/206‑1 (MQ=255) aGGCGATGACGGCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCTGCTGTGGATGTAGACGGTCGCACCTGCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAAcc < 1:159185/236‑1 (MQ=255) tGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGt < 1:453746/235‑1 (MQ=255) cgacgCTGGCCAGATCAGCCTGGGCATCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGc < 2:601988/206‑1 (MQ=255) cgCTGGCCAGATCAGCCTGGGCATCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTc > 2:729124/1‑232 (MQ=255) cTGGCCAGATCAGCCTGGGCATCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACcaaca > 2:633026/1‑189 (MQ=255) aGATCAGCCTGGGCATCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGa > 1:735624/1‑235 (MQ=255) gATCAGCCTGGGCATCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACg < 1:333343/236‑1 (MQ=255) gggCATCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGCGAGCACCTACACCAGCAc > 2:167431/1‑162 (MQ=255) gggCATCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCaaa > 2:766906/1‑232 (MQ=255) tCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGCCGGTGACCGAAGGCGGCGAGATCACCACCACCATCACGCt > 2:28341/1‑160 (MQ=255) cGTCGGCTGTGGATGTAGCCGGTCGCACCTTCGAGAACCTGCAGCTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGc < 1:228433/196‑1 (MQ=255) tCTGCTGTGGATGTAGACGGTCGCACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATcaaca > 1:615421/1‑184 (MQ=255) tCTGCTGTGGATGTAGACGGTCGCACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATcaaca < 2:615419/184‑1 (MQ=255) gtgGATGTAGACGGTCGCACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGc < 1:781964/236‑1 (MQ=255) gTAGACGGTCGCACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAAc < 2:572607/234‑1 (MQ=40) gTAGACGGTCGCACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACg > 1:541668/1‑235 (MQ=255) gTAGACGGTCGCACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCg > 2:12672/1‑165 (MQ=255) caGACGGTCGCACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGt < 1:451541/235‑1 (MQ=39) gACGGTCGCACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGTAc < 1:729126/236‑1 (MQ=255) ggTCGCACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGc < 2:758152/176‑1 (MQ=38) ggTCGCACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGc > 1:758154/1‑176 (MQ=39) gtaccACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAAcc < 1:556056/164‑1 (MQ=37) gTCGCACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCaacaac < 1:247375/167‑1 (MQ=39) cGCACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGTACCGt < 2:735622/233‑1 (MQ=39) cACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTaca > 2:769013/1‑122 (MQ=39) cACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACg > 1:261322/1‑130 (MQ=39) ccTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACCCCACCGACGAAGTTGTGGCCAAGCTCACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGcc < 1:140200/214‑1 (MQ=21) ccTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGc < 1:648119/194‑1 (MQ=18) cTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGTACCGTGGGTa > 2:764767/1‑233 (MQ=38) ttCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTg < 2:600500/210‑1 (MQ=35) gaACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGTACCGTGGGTACTGCTACCg < 1:458634/236‑1 (MQ=39) acccTGCAACGGGGCGGTACCGCTACGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGc < 2:158533/159‑1 (MQ=255) cTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCa > 2:745610/1‑153 (MQ=35) cTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCAc > 2:534628/1‑164 (MQ=37) cTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGCGCGACgg > 1:715654/1‑176 (MQ=25) ggcAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCtt > 2:40045/1‑155 (MQ=35) ggcAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAATGGCACCGTGGGTACTGCCACTGTCACTGCCCCGGACaa > 1:706689/1‑236 (MQ=16) ggcAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCAGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTaat > 2:501839/1‑197 (MQ=19) cGCTTCGGTTCAAGTCATCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACcaca < 2:636797/137‑1 (MQ=37) cTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGTGGCGAGATCACTTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCaacaac < 2:557259/131‑1 (MQ=255) tCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGTACCGTGGGTACTGCTACCGTGACTGCCCCGGACAAc < 2:639678/218‑1 (MQ=40) cACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGTACCGTGGGTACTGCCACCGTCACTGCCCCg > 2:766213/1‑211 (MQ=21) ccaccGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAAc < 2:55382/170‑1 (MQ=33) ccaccGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGTACCGTGGGTACTGCTACCGTGACTGCCCCGGACAACGTCTACGt < 2:353293/217‑1 (MQ=39) cgaAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGTACCGTGGGTACTGCTACCGTGACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTCGTGa > 2:504680/1‑233 (MQ=255) gaAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCAcc > 2:614417/1‑150 (MQ=17) aaGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGTACCGTGGGTACTGCTACCg < 1:187421/183‑1 (MQ=18) tggtggCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGTACCGTGGGTACTGCTACCGTGACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTCGTGATGTCGATc < 1:534671/236‑1 (MQ=40) gtggCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGTACCGTGGGTACTGCTACCGTGACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTCGTg < 1:68634/225‑1 (MQ=39) gCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGTACCGTGGGTACTGCTACCGTGACt < 1:537141/180‑1 (MQ=17) gCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGTACCGTGGGTACTGCTACCGTGACt > 2:157582/1‑180 (MQ=14) gCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGTACCGTGGGTACTGCTACCGTGACt > 2:537139/1‑180 (MQ=14) gCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGTACCGTGGGTACTGCTACCGTGACt < 1:157584/180‑1 (MQ=14) aaGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCg < 2:709797/144‑1 (MQ=34) cTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGTACCGTGGGTACTGCTACCGTGACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTCGTGATGTCGATCGCAACCGtt > 2:446773/1‑234 (MQ=40) gACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGTACCGTGGGCACGGCTACCGTGACTGCCCCGGa > 2:232461/1‑180 (MQ=25) gACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCgtgaag > 2:657683/1‑113 (MQ=255) gcgACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCAc > 1:245607/1‑128 (MQ=35) cGGTAACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAAcc > 2:501016/1‑72 (MQ=21) | CCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGTACCGTGGGTACTGCTACCGTGACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTCGTGATGTCGATCGCAACCGTT > AE015451/206131‑206524 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |