| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 215,395 | A→G | 9.3% | P6967P (CCA→CCG) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 215,395 | 0 | A | G | 9.3% | 157.3 / 3.1 | 61 | P6967P (CCA→CCG) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base A (20/35); new base G (2/4); total (22/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
GCCGGTCAGCACGTCGGTGACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAGGTGGCGAGATCACTTACACCATC > AE015451/215182‑215611 | gCCGGTCAGCACGTCGGTGACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGc < 1:803570/235‑1 (MQ=35)gCCGGGCAGCACGCCGGTGCCCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTgc < 1:203728/228‑1 (MQ=17) cGGTCAGCACGTCGGTGACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGAt < 1:68502/236‑1 (MQ=35) aCGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCCAAACCAGCGCGCCATac > 1:393095/1‑207 (MQ=11) cAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATa > 1:157728/1‑187 (MQ=33) gaaCGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAacg < 2:349319/208‑1 (MQ=38) cAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAacg < 2:361616/209‑1 (MQ=38) aGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCg < 1:558082/236‑1 (MQ=38) cTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAGGGCGATGACGttta < 2:547254/192‑4 (MQ=12) cTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAGGGCGATGACGTTTa > 1:547256/1‑192 (MQ=17) tCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGt < 1:272454/236‑1 (MQ=38) aCCATCCCCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGTGAAACCAGCGCGCCATACAcc < 2:258603/153‑1 (MQ=9) cATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGcc < 1:624900/191‑1 (MQ=38) cATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAACCCGACCTTCACCGTTCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCg < 2:143661/167‑1 (MQ=255) aTCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTggg > 2:685165/1‑203 (MQ=37) cgccgACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCg < 2:557058/209‑1 (MQ=38) ccgACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGaa > 1:718035/1‑236 (MQ=37) cgACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGaaa > 1:752841/1‑236 (MQ=37) aCCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGaa > 2:270786/1‑233 (MQ=35) gACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAg < 1:281750/149‑1 (MQ=35) aCCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAacg < 2:393093/166‑1 (MQ=255) ggTGGCCGAGAACGTCAAGCCGACCTTCACCGTTCACGTCAACCCGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCt < 2:111150/136‑1 (MQ=21) ccGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCg < 1:594632/234‑1 (MQ=17) gagaACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGc < 1:695399/236‑1 (MQ=34) agaACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGc > 2:342531/1‑230 (MQ=34) aCCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTcc > 1:361144/1‑171 (MQ=34) aCCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTcc < 2:361142/171‑1 (MQ=34) ccccGGGCACGGCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCCGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGAt < 2:243585/119‑1 (MQ=255) gTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACACCGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGAt < 1:443632/117‑1 (MQ=25) aCGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCt > 2:152802/1‑161 (MQ=33) gTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGACGTAGCCgg > 1:793131/1‑173 (MQ=21) aaCCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACcgacga < 1:673784/236‑1 (MQ=17) ccAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCg < 2:92207/104‑1 (MQ=35) ccAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTcc < 2:684710/151‑1 (MQ=33) cAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACcgacga < 2:427369/233‑1 (MQ=33) gCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACAACCGACGAagta > 1:4671/1‑235 (MQ=17) gCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGcc > 1:687426/1‑134 (MQ=34) cTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAg < 1:653845/94‑1 (MQ=37) cTGGCCCACGACCGGGGCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCa < 2:159608/122‑1 (MQ=25) aCCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATa > 1:175920/1‑67 (MQ=35) catGAGCAACAGCGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCaa < 1:262601/182‑1 (MQ=18) cTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGc < 2:483893/170‑1 (MQ=255) cTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGc > 1:483895/1‑170 (MQ=255) gAGCAACAACGCCCAGGTCACCAGCAAGGCCGGCGAAACCAGCGCGCCATACAACCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCa < 2:143701/96‑1 (MQ=16) gCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTg > 2:352/1‑104 (MQ=32) aacaacGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAacg > 1:677445/1‑82 (MQ=33) acaacGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGaa > 1:138203/1‑236 (MQ=32) acaacGACCAGGTCACCAACAAGGGCGGCGAAACCCGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGCATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATac < 2:642011/187‑1 (MQ=255) acGCCCAGGTCACCACCAAGGCCGGCGAAACCAGCGCGCCATACACCCCCGCTGCGCAAGGCGATGACGTCTACAACGACGCTGG‑CAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTg < 2:456273/145‑1 (MQ=255) gCCCCGGTCGCCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGGCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCg < 1:146037/128‑1 (MQ=12) cccAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGTGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTg < 2:763620/149‑1 (MQ=17) cccAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAGGTGGc < 1:364692/236‑1 (MQ=31) cAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAGGTGGCga < 1:793339/236‑1 (MQ=31) tCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCAAGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAGGt > 1:553146/1‑227 (MQ=14) aCCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAGGTGGCGAGAt > 2:682832/1‑233 (MQ=31) aTCAAGGCTGGCGAAACCAGCGCGCCGTGCACCCACGCTGCGCAGGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCGCGTCCGCTGTGGATg < 2:152411/106‑1 (MQ=11) cAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCGGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGCCCAAGCTGACcg > 2:612123/1‑197 (MQ=14) ccGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGt > 2:662141/1‑100 (MQ=255) ccGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGt < 1:662143/100‑1 (MQ=16) ccGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAGGTGGCGAGATCACTTACACCATc < 1:268673/236‑1 (MQ=31) ccGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAGGTGGCGAGATCACTTACACCATc < 1:691855/236‑1 (MQ=31) | GCCGGTCAGCACGTCGGTGACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAGGTGGCGAGATCACTTACACCATC > AE015451/215182‑215611 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |