| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 216,994 | C→T | 9.5% | S7500S (AGC→AGT) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 216,994 | 0 | C | T | 9.5% | 156.1 / 5.0 | 52 | S7500S (AGC→AGT) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base C (24/23); new base T (2/3); total (26/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.90e-01 | |||||||||||
AACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGATGTTGGCAAGTTCGAACAGCTGACACTGGACAAGACCCCGGTCAGCACCGCCGTGACCGACGAACCAGGTTCGGGAACTCCAGGCACCGGC > AE015451/216779‑217216 | aaCTCGGCGGTGGCCGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAAc < 1:253812/227‑1 (MQ=255) ggcggTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTg < 1:577826/235‑1 (MQ=255) ttCGAGAACCTCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTg > 2:679050/1‑228 (MQ=255) aaCCTCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCATGCCGGCCAACGGCACCGTGGGCACTGCTACCg < 2:426967/235‑1 (MQ=255) ccTCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCg < 2:287422/233‑1 (MQ=255) gcgcgCTGGGCGGTGCCGCGAAGGTGGATGTCCCCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGtca < 2:254238/188‑1 (MQ=255) ggCGATGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCa < 1:802005/199‑1 (MQ=255) gcgAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTa < 1:774959/236‑1 (MQ=255) aaGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGtca < 1:668273/172‑1 (MQ=255) aaGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACg < 1:730742/235‑1 (MQ=255) agTCACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGcc < 1:317565/210‑1 (MQ=255) acacCACCGACGAAGTGGTGGCCAAGCTGACAGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACAGTCATCACCGTg > 2:381365/1‑164 (MQ=255) gaAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAat > 1:535523/1‑235 (MQ=40) aGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAatcga < 1:400944/236‑1 (MQ=40) ggCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCgg < 1:679052/188‑1 (MQ=39) aaGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGt > 1:710790/1‑236 (MQ=255) aaGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGt > 1:764391/1‑236 (MQ=39) ccGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCCCCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGGGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTc < 2:264569/191‑1 (MQ=255) gcgACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAAc > 1:526202/1‑224 (MQ=255) ccTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGATGt > 1:231108/1‑236 (MQ=255) gcgAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGATGTTGGCAAGTTCGAACAGCTg < 1:502642/236‑1 (MQ=255) gagaTCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGATGTTGGCAAGTTCGAACAGCTg < 2:764389/234‑1 (MQ=255) agaTCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCaaa > 1:427447/1‑185 (MQ=38) cTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGATGTTGGCAAGTTCGAACAGCTg < 2:784199/226‑1 (MQ=255) aTCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGATGTTGGCAAGTTCGAACAGCTGACACTGGACAAGAcccc < 1:721939/236‑1 (MQ=255) cACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGATGTTGGCAAGTTCGAACGGCTGACACTGGACAAGAccc > 1:558869/1‑233 (MQ=255) cccTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGATGTTGGCAAGTTCGAACAGCTGACACTGGACag > 2:426746/1‑225 (MQ=255) cccTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGATGTTGGCAAGTTCGAACAGCTGACACTGGACAAGACCCCGGt > 1:539463/1‑235 (MQ=255) ccTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGATGTTGGCAAGTTCGAACAGCTg < 1:70330/214‑1 (MQ=255) aacaaAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTggcgg > 2:766250/1‑179 (MQ=35) aGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGATGTTGGCAAGTTCGAACAGCTGACACTGGACAAGACCCCGGTCAGCACCGCCGTg > 2:673737/1‑235 (MQ=255) aGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGCCCAACGGCACCGTGGGCACTGCTACCGTCACAGCCCCGGACAACGTCTAc > 2:219513/1‑127 (MQ=17) ccTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGATGtt < 2:535920/177‑1 (MQ=25) cTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGtt > 1:566269/1‑162 (MQ=31) ccAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGCTGTTGGCACGTTCGaa > 2:62392/1‑186 (MQ=255) acaacCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGATGTTGGCAAGTTCGAACAGCTGACACTGGACAAGACCCCGGTCAGCACCGCCGTGACCGACGAACCAGGTTCg < 1:749478/236‑1 (MQ=255) gTGCATTGACCTTCACGCTGAGTGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGATGTTGGCAAGtt < 2:561663/166‑1 (MQ=255) tGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGATGTTGGCAAGTTc > 2:159817/1‑166 (MQ=40) tGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGATGTTGGCAAGTTc < 1:159819/166‑1 (MQ=40) cATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGATGTTGGCAAGTTCGACCAGCTGACACTGGACAAGACCCCGGt > 2:210272/1‑193 (MQ=255) cATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGATGTTGGCAAGTTCGAACAGCTGACACTGGACAAGACCCCGGTCAGCACCGCCGTGACCGACGAACCAGGTTCGGGAACTCCAgg < 1:541110/235‑1 (MQ=255) aTTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGATGTTGGCAAGTTCGAACAGCTGACACTGGACAAGACCCCGGTCAGCACCGCCGTGACCGACGAACCAGGTTCGGGAACTCCAg > 2:724512/1‑233 (MQ=255) tGACCTTCACGCTGAGTGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGATGTTGGCAAGTTCGAACAGCTGACACTgg < 1:303124/177‑1 (MQ=255) tGACCTTCACGCTGAGTGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGATGTTGGCAAGTTCGAACAGCTGACACTgg > 2:303122/1‑177 (MQ=255) ccTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGATGTTGGCAAGTTCGAACAGCTGACACTGGACAAGACCCCGGTCAGCACCGCCGTGACCGACGAACCAGGTTCGGGAACTCCAGGCACCGGc > 1:30946/1‑236 (MQ=255) ttCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGATGTTGGCAAGTTCGAACAGCTg < 2:741106/165‑1 (MQ=255) ttCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGATGTTGGCAAGTTCGAACAGCTg > 1:741108/1‑165 (MQ=255) aCGCTGAGTGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTggcg > 2:520510/1‑133 (MQ=17) aCGCTGAGTGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTggcg < 1:520512/133‑1 (MQ=17) aCGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGATGTTGGCAAGTTCGAACAGCTGACACTGGACAAGACCCCGGTCAGCACCGCCGt > 2:460541/1‑194 (MQ=255) gCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGATGTTGGCAAGTTCGAAc > 2:102407/1‑155 (MQ=255) gCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACCGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGATGTTGGCAAGTTCGAACAg > 2:626464/1‑157 (MQ=255) | AACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGATGTTGGCAAGTTCGAACAGCTGACACTGGACAAGACCCCGGTCAGCACCGCCGTGACCGACGAACCAGGTTCGGGAACTCCAGGCACCGGC > AE015451/216779‑217216 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |