Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	AE015451	1,530,160	C→T	10.2%	R395C (CGT→TGT)  noncoding (49/244 nt)	ftsZ → PP_mr19 →	Cell division protein FtsZ Xoo8 ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	1,530,160	0	C	T	10.2%	210.0 / 11.6	68	R395C (CGT→TGT)  noncoding (49/244 nt)	ftsZ PP_mr19	Cell division protein FtsZ Xoo8 ncRNA
Reads supporting (aligned to +/- strand):  ref base C (24/37);  new base T (3/4);  total (27/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGCACGCATCGAGAAGCCTGTGAAGGTGGTCGACAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGAC  >  AE015451/1529927‑1530388                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                     gcgcACGCATCGAGAAGCCTGTGAAGGTGGTCGACAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGcgtcgt                                                                                                                                                                                                                                    >  1:5312/1‑236 (MQ=255)           cGAGAAGCCTGTGAAGGTGGTCGACAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGAt                                                                                                                                                                                                                           <  1:3045/235‑1 (MQ=255)              gaAGCCTGTGAAGGTGGTCGACAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGAttca                                                                                                                                                                                                                        <  1:528558/235‑3 (MQ=255)                        aaGGTGGTCGACAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTAt                                                                                                                                                                                                              <  1:663389/235‑1 (MQ=255)                        aaGGTGGTCGACAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTAt                                                                                                                                                                                                              <  1:318799/235‑1 (MQ=255)                              gtCGACAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGAt                                                                                                                                                                                                                           <  1:647212/216‑1 (MQ=255)                                 gACAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGtata                                                                                                                                                                                                    >  1:626015/1‑236 (MQ=255)                                      tACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGt                                                                                                                                                                                               <  1:276668/236‑1 (MQ=255)                                       ccccTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTAt                                                                                                                                                                                                              <  1:228794/219‑1 (MQ=255)                                           ggCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTg                                                                                                                                                                                          <  1:231429/235‑1 (MQ=255)                                                aCTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGt                                                                                                                                                                                                       >  1:320370/1‑218 (MQ=255)                                                aCTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTa                                                                                                                                                                                       >  2:425504/1‑234 (MQ=255)                                                     ccAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGTCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCGAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAgg                                                                                                                                                                                                                                >  1:432910/1‑188 (MQ=255)                                                      cagcagGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGGAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGGGATTGGTATTCATCaa                                                                                                                                                                               >  1:179424/1‑236 (MQ=255)                                                       agcagGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCaaa                                                                                                                                                                              >  1:86577/1‑236 (MQ=255)                                                          agGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTAt                                                                                                                                                                                                              <  1:195523/201‑1 (MQ=255)                                                                 cGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAAtt                                                                                                                                                                                                                 <  1:540898/191‑1 (MQ=255)                                                                 cGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAAtt                                                                                                                                                                                                                 >  2:540896/1‑191 (MQ=255)                                                                    aGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTg                                                                                                                                                                   >  2:679475/1‑234 (MQ=255)                                                                         ccAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTatcat                                                                                                                                                            >  1:232095/1‑236 (MQ=255)                                                                               cTGCGCCGGTGCGCCAGGCGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTg                                                                                                                                                                                          <  2:553644/200‑1 (MQ=255)                                                                                   gcCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCtt                                                                                                                                                  >  1:509970/1‑236 (MQ=255)                                                                                          cgcCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGAt                                                                                                                                           >  1:195879/1‑236 (MQ=255)                                                                                           gcCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAgg                                                                                                                                                                            <  2:692529/202‑1 (MQ=255)                                                                                            ccAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTAt                                                                                                                                                                                                              <  2:98640/167‑1 (MQ=255)                                                                                                     gccagcGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGc                                                                                                                                 <  1:574119/235‑1 (MQ=255)                                                                                                          cGGTCAACTACCGTGACCTGGAGCGCACGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAAttt                                                                                                                            <  1:772611/235‑1 (MQ=255)                                                                                                                 cTACCGGGCCCGGGCGCGCCCCACCGTCATGCGCCACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGCCTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGcc                                                                                                                                                    <  2:92944/204‑1 (MQ=255)                                                                                                                         aCCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCaa                                                                                                            <  1:181575/236‑1 (MQ=255)                                                                                                                               aGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATcc                                                                                                                                                          >  1:205953/1‑184 (MQ=255)                                                                                                                                  gcCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAAttt                                                                                                                            <  1:304930/211‑1 (MQ=255)                                                                                                                                        ccGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAaaca                                                                                             <  1:679477/236‑1 (MQ=255)                                                                                                                                            cATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGc                                                                                         <  1:69649/236‑1 (MQ=255)                                                                                                                                            cATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGATAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGc                                                                                         <  1:262974/236‑1 (MQ=255)                                                                                                                                                cgcAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAaacaac                                                                                           <  1:138900/230‑1 (MQ=255)                                                                                                                                                cgcAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCAccc                                                                                     <  1:766829/236‑1 (MQ=255)                                                                                                                                                   aaCCAGGCGCATGCAGGTGCTGCCGCAGACGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGc                                                                                                                                 <  1:715311/189‑1 (MQ=255)                                                                                                                                                        ggCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTgg                                                                                                                                                                        >  1:438117/1‑145 (MQ=255)                                                                                                                                                        ggCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCaa                                                                                                                               >  2:789923/1‑186 (MQ=255)                                                                                                                                                              tGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAg                                                                                                                                                      <  2:721518/157‑1 (MQ=255)                                                                                                                                                              tGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCGGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAaaca                                                                                             <  2:5312/214‑1 (MQ=255)                                                                                                                                                               gCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCAccc                                                                                     <  1:27131/221‑1 (MQ=255)                                                                                                                                                                   gTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATg                                                                                                     <  1:329843/201‑1 (MQ=255)                                                                                                                                                                                 gCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCa                                                                                                             >  2:26315/1‑179 (MQ=255)                                                                                                                                                                                   tAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCa                                                                                                       <  2:342763/183‑1 (MQ=255)                                                                                                                                                                                           aCCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCGGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCAccc                                                                                     <  2:199153/193‑1 (MQ=255)                                                                                                                                                                                              cTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGga                                       >  1:680671/1‑236 (MQ=255)                                                                                                                                                                                                      gACCTGGACTACCTCTATATCCCGGCATTCCTGCGTCGTCACGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCaa                                                                                                            <  1:784799/159‑1 (MQ=255)                                                                                                                                                                                                          tGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTAc                             >  2:415601/1‑234 (MQ=255)                                                                                                                                                                                                           ggACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAaaca                                                                                             <  2:345444/169‑1 (MQ=255)                                                                                                                                                                                                           ggACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAaaca                                                                                             >  1:345446/1‑169 (MQ=255)                                                                                                                                                                                                            gACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGATCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCcac                                                               >  2:112108/1‑198 (MQ=255)                                                                                                                                                                                                             aCTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGAc                        <  1:208717/236‑1 (MQ=255)                                                                                                                                                                                                              cTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTAt                                                                                                                                                                                                              <  2:254631/53‑1 (MQ=255)                                                                                                                                                                                                              cTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGc                                                                  <  2:86576/193‑1 (MQ=255)                                                                                                                                                                                                                aCCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTAc                             <  2:635407/228‑1 (MQ=255)                                                                                                                                                                                                                aCCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTAc                             >  1:635409/1‑228 (MQ=255)                                                                                                                                                                                                                aCCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGAccc                      >  1:751729/1‑235 (MQ=255)                                                                                                                                                                                                                    cGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCAGCCGTg                                                                   >  2:120902/1‑186 (MQ=255)                                                                                                                                                                                                                      atatCCCGGCATTCCTGCGTCGTCAGGCTGCTTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGc                <  1:147307/235‑1 (MQ=255)                                                                                                                                                                                                                         tCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTAt                                                                                                                                                                                                              <  2:747666/42‑1 (MQ=255)                                                                                                                                                                                                                         tCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTAt                                                                                                                                                                                                              >  1:747668/1‑42 (MQ=255)                                                                                                                                                                                                                         tCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTg             >  1:529917/1‑235 (MQ=255)                                                                                                                                                                                                                         tCCCGGCATTACTGCTTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGAATCCATCAAGGGTTGTGTCTGTTATCATCCCCAGCCTTTCTTGATACCTGTTCGCAATTTGCGCTGagg                                                                                                                   >  2:782903/1‑131 (MQ=255)                                                                                                                                                                                                                           ccGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAAttt                                                                                                                            <  2:666122/122‑1 (MQ=255)                                                                                                                                                                                                                              gCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCAcc         >  2:414067/1‑234 (MQ=255)                                                                                                                                                                                                                               cATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGt      <  1:415603/236‑1 (MQ=255)                                                                                                                                                                                                                               cATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGt      <  1:425506/236‑1 (MQ=255)                                                                                                                                                                                                                                      gCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGac  >  2:692907/1‑233 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                     GCGCACGCATCGAGAAGCCTGTGAAGGTGGTCGACAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGAC  >  AE015451/1529927‑1530388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑