Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	AE015451	1,921,549	T→A	9.5%	intergenic (+24/+37)	PP_1720 → / ← PP_1721	Alcohol dehydrogenase, zinc‑containing/putative phosphoserine phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	1,921,549	0	T	A	9.5%	127.4 / 4.7	42	intergenic (+24/+37)	PP_1720/PP_1721	Alcohol dehydrogenase, zinc‑containing/putative phosphoserine phosphatase
Reads supporting (aligned to +/- strand):  ref base T (15/23);  new base A (2/2);  total (17/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01

CAACCGTGATGACGGCTTCAAGGCCGAGCTGTTGCGCGAACTGCAGCAGCAAGTATGGCCGCTGTTCGCCGAGGGGCGGTTGTCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACGGCAAGTCATTCCTCGAATCGGAATAGAAACTCGCGCCCTCCAGGTTTTCCTGCTCCTGATCCAGCCATTCCAGCAACCGCGTG  >  AE015451/1921327‑1921769                                                                                                                                                                                                                               |                                                                                                                                                                                                                             cAACCGTGATGACGGCTTCAAGGCCGAGCTGTTGCGCGAACTGCAGCAGCAAGTATGGCCGCTGTTCGCCGAGGGGCGGTTGTCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAggg                                                                                                                                                                                                                 >  1:30150/1‑236 (MQ=255)  aaCCGTGATGACGGCTTCAAGGCCGAGCTGTTGCGCGAACTGCAGCAGCAAGTATGGCCGCTGTTCGCCGAGGGGCGGTTGTCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCcgc                                                                                                                                                                                                                       >  2:276900/1‑229 (MQ=255)       tgatgaCGGCTTCAAGGCCGAGCTGTTGCGCGAACTGCAGCAGCAAGTATGGCCGCTGTTCGCCGAGGGGCGGTTGTCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCg                                                                                                                                                                                                             >  2:566925/1‑234 (MQ=255)           gaCGGCTTCAAGGCCGAGCTGTTGCGCGAACTGCAGCAGCAAGTATGGCCGCTGTTCGCCGAGGGGCGGTTGTCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGcac                                                                                                                                                                                                         <  2:685673/234‑1 (MQ=255)                      ggCCGAGCTGTTGCGCGAACTGCAGCAGCAAGTATGGCCGCTGTTCGCCGAGGGGCGGTTGTCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCcgcg                                                                                                                                                                                                                      >  2:542696/1‑210 (MQ=255)                             cTGTTGCGCGAACTGCAGCAGCAAGTATGGCCGCTGTTCGCCGAGGGGCGGTTGTCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTc                                                                                                                                                                                        >  2:791198/1‑233 (MQ=255)                                 tGCGCGAACTGCAGCAGCAAGTATGGCCGCTGTTCGCCGAGGGGCGGTTGTCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTcag                                                                                                                                                                                      <  2:504565/231‑1 (MQ=255)                                       aaCTGCAGCAGCAAGTGTGGCCGCTGTTCGCCGAGGGGCGGTTGTCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGca                                                                                                                                                                                                          <  1:735027/205‑1 (MQ=255)                                               cagcaAGTATGGCCGCTGTTCGCCGAGGGGCGGTTGTCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCg                                                                                                                                                                      <  2:701875/233‑1 (MQ=255)                                                      tATGGCCGCTGTTCGCCGAGGGGCGGTTGTCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCAtt                                                                                                                                                            <  1:552044/236‑1 (MQ=255)                                                        tGGCCGCTGTTCGCCGAGGGGCGGTTGTCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCcgc                                                                                                                                                                                                                       <  2:437157/175‑1 (MQ=255)                                                                 ttCGCCGAGGGGCGGTTGTCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCa                                                                                                                                                  <  1:276902/235‑1 (MQ=255)                                                                  tCGCCGAGGGGCGGTTGTCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGGCCCTTCGCGGGTTTACCCGCGAAGGGGCCg                                                                                                                                                                                                             >  2:375638/1‑175 (MQ=255)                                                                         ggggCGGTTGTCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAAcc                                                                                                                                                                                           <  1:566927/186‑1 (MQ=255)                                                                                  gTCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTc                                                                                                                                                                       <  2:293653/197‑1 (MQ=255)                                                                                                     acCTATCCGGTGGAGTTTGCCCAGGTGGCGTATGCCGAGCTGGAGACCAACCAGGGGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTc                                                                                                                                                                       <  2:10391/178‑1 (MQ=255)                                                                                                       cTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGTTTATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCcgcg                                                                                                                                                                                                                      >  2:633143/1‑129 (MQ=255)                                                                                                              gTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATcc                                                                                                     <  1:627474/235‑1 (MQ=255)                                                                                                               tGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTAccc                                                                                                                                                                                                                         <  1:107612/118‑1 (MQ=255)                                                                                                                             gcggcgTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACg                                                                                     <  1:542698/236‑1 (MQ=255)                                                                                                                                gcgTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCAGcccc                                                                                                                                                                                                        <  2:647262/118‑4 (MQ=255)                                                                                                                                 cggaTGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGt                                                                                                          <  2:465717/208‑1 (MQ=255)                                                                                                                                            cTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTcag                                                                                                                                                                                      <  2:502924/124‑1 (MQ=255)                                                                                                                                                       ccaGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTcacc                                                                                                                    <  2:372326/179‑1 (MQ=255)                                                                                                                                                             gtCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACGGCAAGTCATTCCTCGAATCGGAATAGAAACTc                                                     >  1:509986/1‑236 (MQ=255)                                                                                                                                                              gcGGGCCAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCCTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACGGCAAGTCATTCCTCg                                                                      <  1:110530/217‑1 (MQ=255)                                                                                                                                                                    aaGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATACAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAacg                                                                                                                                     >  2:524623/1‑148 (MQ=255)                                                                                                                                                                             agggtgatCGACCCGAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGt                                                                                                          <  2:30150/165‑1 (MQ=255)                                                                                                                                                                                            aGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACGGCAAGTCATTCCTCGAATCGGAATAGAAACTCGCGCCCTCCAGGTTTTCCTGCTCCTGATCCa                      <  1:791200/236‑1 (MQ=255)                                                                                                                                                                                                tGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTAAACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACGGCAAGTCATTCCTc                                                                       >  1:115745/1‑183 (MQ=255)                                                                                                                                                                                                  gtgtAAGCGGGGCTGGCCCCTTCGCGGGTAAACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACGGCAAGTCATTCCTCGa                                                                     <  2:115744/183‑1 (MQ=255)                                                                                                                                                                                                     tAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGCAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCCCGCGCAACACTGCGTCCGGGGTCACCCCATGCggg                                                                                                           >  2:238835/1‑142 (MQ=255)                                                                                                                                                                                                     tAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACGGCAAGTCATTCCTCGAATCGGAATAGAAACTCGCGCCCTCCAGGTTTTCCTGCTCCTGATCCAGCCATTcc              >  1:706907/1‑235 (MQ=255)                                                                                                                                                                                                     tAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACGGCAAGTCATTCCTCGAATCGGAATAGAAACTCGCGCACTCCAgg                                         >  1:491708/1‑208 (MQ=255)                                                                                                                                                                                                      aaGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCCACAACAACGGCAAGTCATTCCTCGAATCGGAATAGAAACTCGCGCCCTCCAGGTTTTCCTGCTCCTGATCCCGCCATTCCAg            >  1:19787/1‑236 (MQ=255)                                                                                                                                                                                                          ggggCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCggg                                                                                                           >  2:696220/1‑137 (MQ=255)                                                                                                                                                                                                               tGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACGGCAAGTCATTCCTCGAATCGGAATAGAAACTCGCGCCCTCCAGGTTTTCCTGCTCCTGATCCAGCCATTCCAGCAACcgcg    >  1:519137/1‑235 (MQ=255)                                                                                                                                                                                                                ggCCCCTTCGCGGGTAAACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACGGCAAGTCATTCCTCGAATCGGAATAGAAACTCGCGCCCTCCAGGTTTTCCTGCTCCTGATCCAGCCATTCCAGCAACCGCGTg  >  1:407404/1‑236 (MQ=255)                                                                                                                                                                                                                    ccTTCGCGGGTTTACCCTCGAAGGGCCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCgg                                                                                                                           <  2:673601/111‑1 (MQ=255)                                                                                                                                                                                                                     cTTCGGGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGt                                                                                                          <  2:54680/127‑1 (MQ=255)                                                                                                                                                                                                                      tgcgcgGGTAAACCCGCGAAGGTGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCa                                                                                                                                                  <  2:15229/84‑1 (MQ=255)                                                                                                                                                                                                                         gcgGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACGGCAAGTCATTCCTCGAATCGGAATAGAAACTCGCGCCCTcc                                            <  2:680244/185‑1 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                             CAACCGTGATGACGGCTTCAAGGCCGAGCTGTTGCGCGAACTGCAGCAGCAAGTATGGCCGCTGTTCGCCGAGGGGCGGTTGTCGCCGCAGCTGGTCGACACCTATCCGGTGGAGTTTGCCCAGGCGGCGTATGCCGAGCTGGAGACCAACCAGGTGTCGGGCAAGCTGGTGATGGTGATCGACCCTAGCCTGGTGTAAGCGGGGCTGGCCCCTTCGCGGGTTTACCCGCGAAGGGGCCGGCACAGCCCATAAAAACCTTCAGCTCCAGCTCAGAGTCGGCCACCCATTGATCTCGGCATGCTCGCGCAACACTGCGTCCGGGTTCACCACATGCGGGTGATCCACTCTCAACAACAACGGCAAGTCATTCCTCGAATCGGAATAGAAACTCGCGCCCTCCAGGTTTTCCTGCTCCTGATCCAGCCATTCCAGCAACCGCGTG  >  AE015451/1921327‑1921769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑