Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AE015451 4,587,695 G→T 8.3% R40R (CGC→CGA) ‡ PP_4063 ← putative Long‑chain‑fatty‑acid‑CoA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE0154514,587,6950GT 8.3% 152.6 / 4.1 45R40R (CGC→CGA) ‡PP_4063putative Long‑chain‑fatty‑acid‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base G (16/25);  new base T (2/2);  total (18/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.62e-01

GCAGCCGGACTGGCGCAACACGTATTCCAGTTCGCCCACACGGTAGGCCGGGTTGATGTTGACCAGAATGGCACCGACCTTGGCACTGGCCAGCTGCAAGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGGTTGTTTTGGGCACGGCTGAAGCAAGCGGGAATACTCTGGCGCAGATTTACGTTTACGTAAA  >  AE015451/4587461‑4587893
                                                                                                                                                                                                                                          |                                                                                                                                                                                                      
gCAGCCGGACTGGCGCAACACGTATTCCAGTGCGCCCACACGGTAGGCCGGGTTGATGTTGACCAGAATGGCACCGACCTTGGCACTGGCCAGCTGCAAGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCtggtgg                                                                                                                                                                                                        <  1:202906/235‑1 (MQ=255)
               cAACACGTATTCCAGTTCGCCCACACGGTAGGCCGGGTTGATGTTGACCAGAATGGCACCGACCTTGGCACTGGCCAGCTGCAAGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCt                                                                                                                                                                                        <  1:625905/236‑1 (MQ=255)
                    cggaTTCCAGTTCGCCCACACGGTGGGCCGGGTTGATGTTGACCAGAATGGCACCGACCTTGGCACTGGCCAGCTGCAAGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGc                                                                                                                                                                                     <  2:729847/231‑1 (MQ=255)
                           cAGTTCGCCCACACGGTAGGCCGGGTTGATGTTGACCAGAATGGCACCGACCTTGGCACTGGCCAGCTGCAAGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCg                                                                                                                                                                                <  2:673501/232‑1 (MQ=255)
                                       acGGTAGGCCGGGTTGATGTTGACCAGAATGGCACCGACCTTGGCACTGGCCAGCTGCAAGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACgg                                                                                                                                                                <  1:273997/236‑1 (MQ=255)
                                                                               ttGGCACTGGCCAGCTGCAAGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCCGGGCCCCGCTGTCGCAACCGCGGGCCACgggg                                                                                                                                                              >  2:100048/1‑196 (MQ=255)
                                                                               ttGGCACTGGCCAGCTGCAAGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGAt                                                                                                                                          >  2:625659/1‑218 (MQ=255)
                                                                                  gCACTGGCCAGCTGCAAGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACgg                                                                                                                                                                <  1:770421/193‑1 (MQ=255)
                                                                                   cACTGGCCAGCTGCAAGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCaa                                                                                                                           >  2:659493/1‑229 (MQ=255)
                                                                                   cACTGGCCAGCTGCAAGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCaa                                                                                                                           <  1:659495/229‑1 (MQ=255)
                                                                                    aCTGGCCAGCTGCAAGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACCCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGggtcggt                                                                                                                   >  1:419218/1‑236 (MQ=255)
                                                                                       ggCAAGCTGCAAGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTgg                                                                                                                                               <  1:151112/205‑1 (MQ=255)
                                                                                           aGCTGCAAGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGt                                                                                                                                 >  2:609671/1‑215 (MQ=255)
                                                                                             cTGCAAGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAg                                                                                                                                                     <  1:682425/193‑1 (MQ=255)
                                                                                                           acTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGTCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCAGTCGCAACACCGGGCCACGGTGGCATCGAAGGCCTGGCCGATg                                                                                                                                         <  2:496607/191‑1 (MQ=255)
                                                                                                           acTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCATGGCCGATGGTCTGGGTCAgaa                                                                                                                            >  1:71902/1‑202 (MQ=255)
                                                                                                             tGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTc                                                                                            <  1:269876/234‑1 (MQ=255)
                                                                                                                  gcAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCt                                                                                                                                                                                        >  2:550718/1‑137 (MQ=255)
                                                                                                                  gcAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGggtcg                                                                                                                     >  2:138709/1‑204 (MQ=255)
                                                                                                                     gTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTGCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGGTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGaca                                                                                                                                                                                                 <  2:261461/125‑1 (MQ=255)
                                                                                                                         ggCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTc                                                                                            <  1:226679/222‑1 (MQ=255)
                                                                                                                            gACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGggtcggtc                                                                                                                  >  2:277624/1‑197 (MQ=255)
                                                                                                                                aGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGATCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGCCACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGCCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGAc                                                                                     >  1:441556/1‑222 (MQ=255)
                                                                                                                                         cacGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGgt                                                                             <  1:27671/221‑1 (MQ=255)
                                                                                                                                            ggTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCa                                                                                                                                                           >  1:471381/1‑140 (MQ=255)
                                                                                                                                               cGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGGTTGttt                                                         >  1:282474/1‑235 (MQ=255)
                                                                                                                                                  cGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGGTTGTTTTgg                                                      <  1:138711/235‑1 (MQ=255)
                                                                                                                                                    ttgtgCACGCCCAGTGCGGGCAGGGCGCGGGCGGAGATTTGGACCTGGTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTc                                                                                                                                                                                       <  2:15176/103‑1 (MQ=255)
                                                                                                                                                             cccAGGGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACGGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTc                                                                                            <  1:70188/186‑1 (MQ=255)
                                                                                                                                                                  tGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTc                                                                                            <  2:486454/181‑1 (MQ=255)
                                                                                                                                                                    cGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGGTTGTTTTGGGCACGGCTGAAGCAAGCgg                                   >  1:401745/1‑236 (MQ=255)
                                                                                                                                                                            gcgcGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCTATGGTCTGGGTCAGCAAGGGTCGGTCCTGGc                                                                                                             <  2:423263/154‑1 (MQ=255)
                                                                                                                                                                               cgGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGAGTCATGGGTGTCCCTTCTTGTGGTTGTTTTGGGCACGGCTGAAGCAAGCGGGAATACTCTg                         <  1:439510/235‑1 (MQ=255)
                                                                                                                                                                                 ggCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACCCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGACGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGGTTGTTTTGGGCACGGCTGatgca                                        <  2:419216/218‑1 (MQ=255)
                                                                                                                                                                                        atTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGt                                                                                                                                                                                  <  1:688243/73‑1 (MQ=255)
                                                                                                                                                                                        atTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGt                                                                                                                                                                                  >  2:688241/1‑73 (MQ=255)
                                                                                                                                                                                               ccTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGTCCCGCCACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGa                                                                                                           <  2:487356/137‑1 (MQ=255)
                                                                                                                                                                                               ccTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGTCCCGCCACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGa                                                                                                           >  1:487358/1‑137 (MQ=255)
                                                                                                                                                                                                cTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGGTTGTTTTGGGCACGGCTGAAGCAAGCGGGAATACTCTGGCGCAGAtttacgtttac       <  1:751206/236‑1 (MQ=255)
                                                                                                                                                                                                 tGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGGTTGTTTTGGGCACGGCTGAAGCAAGCGGGAATActca                          >  2:615752/1‑215 (MQ=255)
                                                                                                                                                                                                     cGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGgt                                                                             <  2:611709/161‑1 (MQ=255)
                                                                                                                                                                                                                cgccAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTgg                                                               >  2:416014/1‑164 (MQ=255)
                                                                                                                                                                                                                  ccAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTc                                                                                            <  1:77122/133‑1 (MQ=255)
                                                                                                                                                                                                                         tAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGGTTGTTTTgg                                                      <  2:806671/164‑1 (MQ=255)
                                                                                                                                                                                                                                   ccTGGTGTCCCGCCACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGGTTGTTTTGGGCACGGCTGAAGCAAGCGGGAATACTCTGGCGCAGATTTACGTTTACGTaaa  >  2:371354/1‑206 (MQ=255)
                                                                                                                                                                                                                                   ccTGGTGTCCCGCCACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGGTTGTTTTGGGCACGGCTGAAGCAAGCGGGAATACTCTGGCGCAGATTTACGTTTACGTaaa  <  1:371356/206‑1 (MQ=255)
                                                                                                                                                                                                                                         ggCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGGTTGTTTTGGGCACGGCTGAAGCAAGCGGGAATACTCTGGCGCAGAttta              <  2:522242/188‑1 (MQ=255)
                                                                                                                                                                                                                                         ggCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGGTTGTTTTGGGCACGGCTGAAGCAAGCGGGAATACTCTGGCGCAGAttta              >  1:522244/1‑188 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                      
GCAGCCGGACTGGCGCAACACGTATTCCAGTTCGCCCACACGGTAGGCCGGGTTGATGTTGACCAGAATGGCACCGACCTTGGCACTGGCCAGCTGCAAGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCATCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGGTTGTTTTGGGCACGGCTGAAGCAAGCGGGAATACTCTGGCGCAGATTTACGTTTACGTAAA  >  AE015451/4587461‑4587893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑