Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AE015451 4,635,590 T→C 59.1% K56E (AAA→GAA)  uvrY ← two‑componenent system BarA/UvrY ‑ regulatory subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE0154514,635,5900TC59.1% 24.0 / 51.3 44K56E (AAA→GAA) uvrYtwo‑componenent system BarA/UvrY ‑ regulatory subunit
Reads supporting (aligned to +/- strand):  ref base T (10/8);  new base C (13/13);  total (23/21)
Fisher's exact test for biased strand distribution p-value = 7.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.14e-01

CAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTC  >  AE015451/4635357‑4635809
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                           
cAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTc                                                                                                                                                                                                                            <  1:610668/235‑1 (MQ=255)
                                      cGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGc                                                                                                                                                                                              <  2:616236/227‑1 (MQ=255)
                                      cGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGc                                                                                                                                                                                              >  1:616238/1‑227 (MQ=255)
                                      cGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCggtc                                                                                                                                                                                      >  1:680362/1‑233 (MQ=255)
                                      cGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCAACCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCAtttt                                                                                                                                                                                                                             >  2:795357/1‑196 (MQ=255)
                                       ggCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTCCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCg                                                                                                                                                                                         >  1:398128/1‑231 (MQ=255)
                                       ggCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTCCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCa                                                                                                                                                                                    >  1:757487/1‑236 (MQ=255)
                                       ggCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTCCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCa                                                                                                                                                                                    >  1:685160/1‑236 (MQ=255)
                                                           ccTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTCCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGc                                                                                                                                                                  >  2:298469/1‑234 (MQ=255)
                                                                            aGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGt                                                                                                                                                                                  >  2:621239/1‑201 (MQ=255)
                                                                                              aGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACCCCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTCCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAg                                                                                                                                                                             >  1:27243/1‑188 (MQ=255)
                                                                                                                     caacaaGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTCCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCg                                                                                                              <  1:800605/228‑1 (MQ=255)
                                                                                                                             gtgtGGGGAACGGGTCTTCCTCGCACAACGTCACCGCCACTACCTTGATGTCCGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTg                                                                                                                                         >  2:305480/1‑193 (MQ=255)
                                                                                                                                tgGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTCCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAAtctc                                                                                                                                                         <  1:651584/174‑1 (MQ=255)
                                                                                                                                  gggAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTCCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTa                                                                                             >  2:756174/1‑232 (MQ=255)
                                                                                                                                   ggAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTCCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGa                                                                                        <  1:323992/236‑1 (MQ=255)
                                                                                                                                        gggTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTCCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGtc                                                                                   >  2:564975/1‑235 (MQ=255)
                                                                                                                                        gggTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTCCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGt                                                                                    >  1:283386/1‑235 (MQ=255)
                                                                                                                                                cTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGACTCTCCCTCACCCACCACCTGCAGGCCGTCGATGt                                                                                                                           >  2:253389/1‑188 (MQ=255)
                                                                                                                                                cTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTCCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCa                                                                                          >  2:68205/1‑221 (MQ=255)
                                                                                                                                                       acCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAAtctc                                                                                                                                                         <  2:366095/151‑1 (MQ=255)
                                                                                                                                                            cgccGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTGTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTCCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCaa                                                                                                                    <  1:130419/181‑1 (MQ=255)
                                                                                                                                                            cACCGCCACCACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTCCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCgtgt                                                                                                           >  1:253630/1‑192 (MQ=255)
                                                                                                                                                             cccGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTCCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCt                                                              <  1:170513/235‑1 (MQ=255)
                                                                                                                                                              ccGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTAcc                                                                                           >  2:642493/1‑206 (MQ=255)
                                                                                                                                                               cGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTa                                                             <  1:795359/235‑1 (MQ=255)
                                                                                                                                                                        cTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTCCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTaa                                                                                                         <  1:781396/182‑1 (MQ=255)
                                                                                                                                                                           gaggTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTCCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCg                                                                                                                                                                <  2:24611/121‑1 (MQ=255)
                                                                                                                                                                            aTGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTCCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGt                                                                                    >  1:744784/1‑199 (MQ=255)
                                                                                                                                                                             tGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCCGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGca                                                     <  1:167930/229‑1 (MQ=255)
                                                                                                                                                                                 aGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCt                                                              <  1:666495/216‑1 (MQ=255)
                                                                                                                                                                                   ggTGGCTGCGTAGCAGTTTGCGGGTGGTCTCCAGGCCGCCGATGCCGGGCATTTCCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCAAGTTTCAGTGCCGACTCGCAGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATcc                                                                                                               <  2:440556/165‑1 (MQ=255)
                                                                                                                                                                                    gTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTcag                                        >  1:791793/1‑235 (MQ=255)
                                                                                                                                                                                         tGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTCCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGc                                  <  1:68206/236‑1 (MQ=255)
                                                                                                                                                                                          gcgcAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCACTCTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGCGCCCGtt                                                                                                                                                                                 >  2:216471/1‑92 (MQ=255)
                                                                                                                                                                                           cgcAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTCCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTaa                                                                                                         <  2:467670/163‑1 (MQ=255)
                                                                                                                                                                                           cgcAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTCCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACACt                                                              >  1:793284/1‑206 (MQ=255)
                                                                                                                                                                                                     tGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTCCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCaa                                                        <  1:352278/202‑1 (MQ=255)
                                                                                                                                                                                                        gggTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGccc                                                                                                                     >  2:137993/1‑137 (MQ=255)
                                                                                                                                                                                                               cgcctggCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCacacac                                                <  1:139324/195‑1 (MQ=255)
                                                                                                                                                                                                                tCCAGGCCGCCGATGCCGGGCATTTCCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCaa                                                                                                                    >  1:804694/1‑131 (MQ=255)
                                                                                                                                                                                                                tCCAGGCCGCCGATGCCGGGCATTTCCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCaa                                                                                                                    <  2:804692/131‑1 (MQ=255)
                                                                                                                                                                                                                     gccgccGATGCCGGGCATTTTCACATCCATCAGCACAACGGCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAAtt      <  1:621241/236‑1 (MQ=255)
                                                                                                                                                                                                                         ccgATGCCGGGCATTTCCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTACCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGCCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGAcc                                                                         <  2:358297/165‑1 (MQ=255)
                                                                                                                                                                                                                            aTGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTc  >  2:721881/1‑233 (MQ=255)
                                                                                                                                                                                                                                         tCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCaa                                                                                                                    <  1:160315/106‑1 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                           
CAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGGTTCGTACCAGATCGTGATCATCGACCACTAAGACCCTAATCAAGCACACACCTCGTCAGCAGGGCGATTGGATCCCGCCACCTTAACAAAATTTTTC  >  AE015451/4635357‑4635809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑