Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AE015451 1,499,480 2 bp→AC 100% intergenic (+57/‑106) trpS → / → zapE Tryptophan‑‑tRNA ligase/putative enzyme with nucleoside triphosphate hydrolase domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE0154511,499,4800CA100.0% 107.5 / ‑6.8 35intergenic (+57/‑107)trpS/zapETryptophan‑‑tRNA ligase/putative enzyme with nucleoside triphosphate hydrolase domain
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (8/26);  minor base G (0/1);  total (8/27)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
*AE0154511,499,4810AC100.0% 103.6 / NA 35intergenic (+58/‑106)trpS/zapETryptophan‑‑tRNA ligase/putative enzyme with nucleoside triphosphate hydrolase domain
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (8/27);  total (8/27)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑T‑CGGGCACG‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGG  >  AE015451/1499273‑1499695
                                                                                                                                                                                                               ||                                                                                                                                                                                                                          
tGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAcg‑c                                                                                                                                                                                               <  1:487566/236‑1 (MQ=255)
                               aGCTCCGGCCTGTGGCTCACTGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAcg‑c                                                                                                                                                                                               <  1:101707/205‑1 (MQ=38)
                                                cAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGcc                                                                                                                                                             <  1:309814/222‑1 (MQ=255)
                                                   gggCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGGGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCCC‑GCTCCCACAGAc                                                                                                                                                                                 <  2:792600/198‑1 (MQ=37)
                                                                 ccGACGGGCGGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACGGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAcg‑c                                                                                                                                                                                               <  2:95702/171‑1 (MQ=37)
                                                                           gCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCg                                                                                                                                                                                                                 >  2:82424/1‑145 (MQ=255)
                                                                            cAGTTCGCCAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcg                                                                                                                                                                                                        <  1:746642/153‑5 (MQ=255)
                                                                                               cccGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGGGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgc                                                                                                                                                                                                         <  2:507797/133‑4 (MQ=255)
                                                                                                       gCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc                                                                                         <  1:728461/235‑1 (MQ=39)
                                                                                                             aTTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCCC‑GCTCCCACAGACTGATTGCCCTTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGc                                                                                                                             >  1:220505/1‑193 (MQ=37)
                                                                                                                 agagCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTc                                                                                                                          <  2:220503/192‑1 (MQ=37)
                                                                                                                  gagCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTT‑CGCGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATc                                                                                <  2:311518/233‑1 (MQ=255)
                                                                                                                     ccTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgt                                                                                                                                <  2:688947/182‑1 (MQ=37)
                                                                                                                     ccTGAACGAAGCCGTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAcg‑c                                                                                                                                                                                               <  2:241790/119‑1 (MQ=21)
                                                                                                                        gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGAc                                                                                                                                              >  1:667224/1‑165 (MQ=25)
                                                                                                                            gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTGTTGTTGCCTCGCTAACGa                                                                                                                  >  2:703628/1‑189 (MQ=37)
                                                                                                                                         cacaGCAGGACGGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGGGCATGGCACCGGCTGTGCCGGTGTTCG‑CGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa                                                                                                   <  2:23683/191‑1 (MQ=37)
                                                                                                                                               agGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGc                                                                                     >  1:7882/1‑199 (MQ=37)
                                                                                                                                                   cTGAGGCTGCCCAACGGTGGGGGCGGGCGTGCCCGCGAAACAGGCGCCGCGGGGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAcg‑c                                                                                                                                                                                               <  2:256/89‑1 (MQ=17)
                                                                                                                                                   cTGAGGCGGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa                                                                                                              <  2:217445/170‑1 (MQ=25)
                                                                                                                                                     gcggCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCCC‑GCTCCCACAGACt                                                                                                                                                                                <  2:46456/100‑1 (MQ=11)
                                                                                                                                                      cggCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCGCCGGCTTTGCCGGTGTTCG‑CGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa                                                                                                   <  1:144636/177‑1 (MQ=37)
                                                                                                                                                      cggCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTT‑CGCGGGCACG‑CCAGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa                                                                                                              <  2:307070/166‑1 (MQ=25)
                                                                                                                                                        gCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAAt                                                                                                                <  1:709338/163‑1 (MQ=25)
                                                                                                                                                               aaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGt                                  >  2:294428/1‑234 (MQ=38)
                                                                                                                                                                   gTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGcc                                                                                                                                                             <  2:278403/107‑1 (MQ=14)
                                                                                                                                                                   gTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGcc                                                                                                                                                             >  1:278405/1‑107 (MQ=14)
                                                                                                                                                                          cGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa                                                                                                   <  1:120452/158‑1 (MQ=37)
                                                                                                                                                                                 gCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTT‑CGCGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTg               >  1:712685/1‑235 (MQ=255)
                                                                                                                                                                                  cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTa             <  1:799684/236‑1 (MQ=255)
                                                                                                                                                                                              gcgcCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCGCGCCC‑GCTCCCACAGACTGATTGCCATTAAGCgg                                                                                                                                                                <  2:231280/77‑1 (MQ=11)
                                                                                                                                                                                              gcgcCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTgg  <  1:82425/235‑1 (MQ=255)
                                                                                                                                                                                                  cgcgGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTcc                       <  1:391548/210‑1 (MQ=39)
                                                                                                                                                                                                   gcgGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGACTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAAt                                                                                                                <  2:369062/120‑1 (MQ=25)
                                                                                                                                                                                                       tGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTc                        <  2:667222/204‑1 (MQ=39)
                                                                                                                                                                                                               ||                                                                                                                                                                                                                          
TGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑T‑CGGGCACG‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGG  >  AE015451/1499273‑1499695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑