Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	AE015451	278,742:1	+A	100%	intergenic (‑237/+77)	PP_0224 ← / ← sctC	Monooxygenase, DszC family/sulfur compound ABC transporter ‑ ATP‑binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	278,742	1	.	A	100.0%	141.2 / NA	39	intergenic (‑237/+77)	PP_0224/sctC	Monooxygenase, DszC family/sulfur compound ABC transporter ‑ ATP‑binding subunit
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (27/12);  total (27/12)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CACTCAATGCCCTGGCGTTACGCACTGGGGTGATTGTGATCCTGACCATTCCTGACCTCACAAAATAGTTATGTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGT‑AACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCTGCAAACAGGGCCTTGGCCTCGCCTTGCTCCACGATCACGCCTTTGTCGAAGAAAATCACCCGGTTC  >  AE015451/278525‑278951                                                                                                                                                                                                                           |                                                                                                                                                                                                                  cACTCAATGCCCTGGCGTTACGCACTGGGGTGATTGTGATCCTGACCATTCCTGACCTCACAAAATAGTTATGTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTacaca                                                                                                                                                                                                   <  1:145840/235‑1 (MQ=255)                  ttACGCAGTGGGGTGATTGTGATCCTGACCATTCCTGACCTCACAAAATAGTTATGTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTacaca                                                                                                                                                                                                   <  2:510706/218‑1 (MQ=255)                    aCGCACTGGGGTGATTGTGATCCTGACCATTCCTGACCTCACAAAATAGTTATGTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGT‑aa                                                                                                                                                                                                                 <  2:307783/201‑1 (MQ=255)                       cACTGGGGTGATTGTGATCCTGACCATTCCTGACCTCACAAAATAGTTATGTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTacaca                                                                                                                                                                                                   <  1:394663/213‑1 (MQ=255)                                               cATTCCTGACCTCACAAAATAGTTATGTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCAGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCggg                                                                                                                                                                  >  2:414283/1‑222 (MQ=255)                                                aTTCCTGACCTCACAAAATAGTTATGTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACAAATGCCTCAAGATCTGTGCCCTGTAGGAGCGGGTTTACCCGCGAAAgg                                                                                                                                                   >  1:49838/1‑236 (MQ=255)                                                  tCCTGACCTCACAAAATAGTTATGTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCtgtg                                                                                                                                                                                 >  2:414571/1‑204 (MQ=255)                                                               aaaTAGTTATGTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGc                                                                                                                                                                                              <  2:344905/178‑1 (MQ=255)                                                               aaaTAGTTATGTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGc                                                                                                                                                                                              >  1:344909/1‑178 (MQ=255)                                                               aaaTAGTTATGTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGa                                                                                                                                                                       >  1:229185/1‑201 (MQ=255)                                                                               aCAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTacaca                                                                                                                                                                                                   >  1:32079/1‑157 (MQ=255)                                                                                cAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCgg                                                                                                                                              >  1:507002/1‑209 (MQ=255)                                                                                         gcgACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCa                                                                                                                                                                               >  2:240363/1‑167 (MQ=255)                                                                                         gcgACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGT‑aaac                                                                                                                                                                                                               <  2:231632/134‑3 (MQ=255)                                                                                         gcgACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGT‑aaac                                                                                                                                                                                                               >  1:231636/1‑132 (MQ=255)                                                                                                                       gtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCtg                                                                                                                                                                                   >  2:20690/1‑133 (MQ=255)                                                                                                                                  cTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCg                                                                         >  1:222691/1‑228 (MQ=255)                                                                                                                                    aaTGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGaaa                                                                                                                                                     >  2:456897/1‑150 (MQ=255)                                                                                                                                    aaTGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTCTGCACTGTAGGAGCGGGTTTACCCGCGa                                                                                                                                                       >  1:416397/1‑148 (MQ=255)                                                                                                                                       gATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATGTGTGCGGTGTAGGAGCggg                                                                                                                                                                  >  1:176185/1‑134 (MQ=39)                                                                                                                                       gATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACAGGTTTACTCGGAGGc                                                                                                                           >  1:265962/1‑173 (MQ=255)                                                                                                                                       gATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTcttc                                                                              <  1:251454/218‑1 (MQ=255)                                                                                                                                       gATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCTGCAAACAg                                                            >  1:364858/1‑236 (MQ=255)                                                                                                                                       gATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATATGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTcttc                                                                              >  2:251450/1‑218 (MQ=255)                                                                                                                                        aTTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGTCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAAc                                                                                                           >  1:312868/1‑188 (MQ=255)                                                                                                                                        aTTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTccc                                                                                                                  >  2:3598/1‑181 (MQ=255)                                                                                                                                                                 tttttGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGTCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCTGCAAACAGGGCCTTGGCCTc                                                <  1:3598/222‑1 (MQ=255)                                                                                                                                                                        aaTATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCgg                                                                        >  2:290266/1‑191 (MQ=255)                                                                                                                                                                        aaTATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCgg                                                                        <  1:290270/191‑1 (MQ=255)                                                                                                                                                                        aaTATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCTGCAAACAGGGCCTTGGCCTCGCCTTGCTCCACGATCACGc                            >  1:456603/1‑235 (MQ=255)                                                                                                                                                                            tttATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCTGCAAACAgg                                                           <  1:414575/200‑1 (MQ=255)                                                                                                                                                                            tttAGAGCAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCt                                                                                                                                                                                    <  2:12039/79‑1 (MQ=25)                                                                                                                                                                                        ggATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGc                                                                                                                                                                                              >  2:124851/1‑57 (MQ=21)                                                                                                                                                                                        ggATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGc                                                                                                                                                                                              <  1:124855/57‑1 (MQ=21)                                                                                                                                                                                        ggATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCTGCAAACAGGGCCTTGGCCTCGCCTTGCTCCACGATCACGCCTTTGTCgaa                  >  1:106985/1‑229 (MQ=255)                                                                                                                                                                                         gATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCCAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGtc                                                                                                                                      >  2:7708/1‑111 (MQ=255)                                                                                                                                                                                         gATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCg                                                                                                                         <  1:464317/125‑1 (MQ=255)                                                                                                                                                                                         gATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCg                                                                                                                         >  2:464313/1‑125 (MQ=255)                                                                                                                                                                                         gATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGGTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCTGCAAACAg                                                            >  1:189749/1‑186 (MQ=255)                                                                                                                                                                                           tttGTTACAGAGGTGCCGGGCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTcttc                                                                              >  2:343091/1‑166 (MQ=255)                                                                                                                                                                                                 aCAGAGGTGCCGGGCTCTTCGCAGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCTGCAAACAGGGCCTTGGCCTCGCCTTGCTCCACGATCACGCCTTTGTCGAAGAAAATCACCCGGTTc  <  1:456901/236‑1 (MQ=255)                                                                                                                                                                                                             ggCTCTTCGCGGGTAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGCACTGCCGCGTACGCTCTTCCTTCGGCGCTGCAAACAGGGc                                                         >  1:267550/1‑169 (MQ=255)                                                                                                                                                                                                                           |                                                                                                                                                                                                                  CACTCAATGCCCTGGCGTTACGCACTGGGGTGATTGTGATCCTGACCATTCCTGACCTCACAAAATAGTTATGTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGT‑AACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGGCGCTGCAAACAGGGCCTTGGCCTCGCCTTGCTCCACGATCACGCCTTTGTCGAAGAAAATCACCCGGTTC  >  AE015451/278525‑278951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑