Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	AE015451	5,201,568	Δ98 bp	37.4%	intergenic (‑54/‑29)	PP_4582 ← / → PP_4583	conserved membrane protein of unknown function/putative Peptidase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 5201567	12 (0.470)	5 (0.200)	5/312	NT	37.4%	intergenic (‑53/‑127)	PP_4582/PP_4583	conserved membrane protein of unknown function/putative Peptidase
	?	AE015451	5201666 =	5 (0.200)					intergenic (‑152/‑28)	PP_4582/PP_4583	conserved membrane protein of unknown function/putative Peptidase

ACGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/5201357‑5201567 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggcACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCG  >  AE015451/5201666‑5201848                                                                                                                                                                                                                                                                                                                                                                                                            ACGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGG                                                                                                                                                                                           <  1:418976/209‑1                                       GCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTGGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGG                                                                                                                                                                                           <  1:500401/171‑1                                                                                                       CCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCG                                                                                   <  1:407704/211‑1                                                                                                                               CCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCC                                                                           <  2:443855/195‑1                                                                                                                               CCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCC                                                                                                                <  1:497331/158‑1                                                                                                                                                    TTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGCCC                                                                                                                                              <  1:29799/107‑1                                                                                                                                                                                              TATGATAAACACTTTTCCGGGCACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCG  >  2:419649/1‑205                                                                                                                                                                                                            agCCGGGCACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACA                                                                                      <  1:274320/105‑1                                                                                                                                                                                                             ggCGGGCACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCG                                                                                   <  1:477612/107‑1                                                                                                                                                                                                               CGGGCACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCC                            <  1:55821/162‑1                                                                                                                                                                                                                                                                                                                                                                                                            ACGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/5201357‑5201567 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggcACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCG  >  AE015451/5201666‑5201848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.