New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	459279 =	26 (1.010)	3 (0.120)	3/298	NT	8.7%	coding (576/912 nt)	pqqB	Coenzyme PQQ synthesis protein B
	?	AE015451	459831 =	39 (1.620)					coding (24/912 nt)	pqqB	Coenzyme PQQ synthesis protein B

CGCGGGAAGCCGTCGAGCACTTCCAGCATGCCGCCAGGGCCGTTCTGGGCCAGGTGACCCATCTCGCGGCCGGTGCGGGTGCCCACGCCACGGCGCTGCATTTCGTCGTCTTCCCACAGGGTGCCGTCGACCAGCAGGCAGTCGGCACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATAAGGCGGCGCGGCGCTGCGCAGGGGGAAGGGAGTGAACTTGAGGTTGGGGCAGGCGTCGATCACGAAGCTGCCTTCCAGCTCGATACGGTTCCACTGCAGGCCACCGTTCCAGTGGCTGAGCATGTTGAACAGCGGGAAACCGGTGGTC  >  AE015451/459070‑459501                                                                                                                                                                                                                  |                                                                                                                                                                                                                               gaacaacccaaccctcattcggaaggagtaattccatgtggaccaaacctgcatacactgacctgcgtatcggcttcgaagtcaccatgtacttcgctaaccgctaagccaggcttagggtttcacgcctcggcctgccggggcgttttcgttttttcgatccggtttctgcatagagagtggccatgtacatccaggttctcggctccGCCGCCGGTg                                                                                                                                                                                                                       <  2:160592‑M1/10‑1 (MQ=255)                  ttcggaaggagtaattccatgtggaccaaacctgcatacactgacctgcgtatcggcttcgaagtcaccatgtacttcgctaaccgctaagccaggcttagggtttcacgcctcggcctgccggggcgttttcgttttttcgatccggtttctgcatagagagtcgccatgtacatccaggttctcggctccgc                                                                                                                                                                                                                               <  2:494673‑M1/2‑1 (MQ=255)                                   cAGGGCCGTTCTGGGCCAGGTGACCCATCTCGCGGCCGGTGCGGGTGCCCACGCCACGGCGCTGCATTTCGTCGTCTTCCCACAGGGTGCCGTCGACCAGCAGGCAGTCGGCACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGaa                                                                                                                                                                     <  1:458535/235‑1 (MQ=255)                                             cggggCCAGGTGACCCATCTCGCGGCCGGTGCGGGTGCCCACGCCACGGCGCTGCATTTCGTCGTCTTCCCACAGGGTGCCGTCGACCAGCAGGCAGTCGGCACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCt                                                                                                                                                                                                              <  1:16498/182‑1 (MQ=255)                                                 ctgcatacactgacctgcgtatcggcttcgaagtcaccatgtacttcgctaaccgctaagccaggcttagggtttcacgcctcggcctgccggggcgttttcgttttttcgatccggtttctgcatagagagtggccatgtacatccaggttctcggctccGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATa                                                                                                                                                      >  1:155320‑M1/162‑236 (MQ=255)                                                      gTGACCCATCTCGCGGCCGGTGCGGGTGCCCACGCCACGGCGCTGCCTTTCGTCGTCTTCCCACAGGGTGCCGTCGACCAGCAGGCAGTCGGCACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATc                                                                                                                                                                                       <  2:192242/198‑1 (MQ=255)                                                        gACCCATCTCGCGGCCGGTGCGGGTGCCCACGCCACGGCGCTGCATTTCGTCGTCTTCCCACAGGGTGCCGTCGACCAGCAGGCAGTCGGCACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGGGTGGCGAATAAggcgg                                                                                                                                                >  1:384128/1‑235 (MQ=255)                                                            cctctcGCGGCCGGTGCGGGTGCCCACGCCACGGCGCTGCATTTCGTCGTCTTCCCACAGGGTGCCGTCGACCAGCAGGCAGTCGGCACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATAAGgcggcgcgg                                                                                                                                           <  1:434423/234‑1 (MQ=255)                                                                  gcgGCCGGTGCGGGTGCCCACGCCACGGCGCTGCATTTCGTCGTCTTCCCACAGGGTGCCGTCGACCAGCAGGCAGTCGGCACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCaaa                                                                                                                                                                                                  <  2:19005/175‑1 (MQ=255)                                                                              ggTGCCCACGCCACGGCGCTGCATTTCGTCGTCTTCCCACAGGGTGCCGTCGACCAGCAGGCAGTCGGCACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATAAggcg                                                                                                                                                 <  2:326146/212‑1 (MQ=255)                                                                                tGCCCACGCCACGGCGCTGCATTGCGTCGTCTTCCCACAGGGTGCCGTCGACCAGCAGGCAGTCGGCACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCg                                                                                                                                                                       <  1:280264/188‑1 (MQ=255)                                                                                       gccacgGCGCTGCATTTCGTCGTCTTCCCACAGGGTGCCGTCGACCAGCAGGCAGTCGGCACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATAAGGCGGCGCGGCGCTGCGCAGGGGGAAgggg                                                                                                                       >  2:186755/1‑228 (MQ=255)                                                                                            ggCGCTGCATTTCGTCGTCTTCCCACAGGGTGCCGTCGACCAGCAGGCAGTCGGCACCGTGCATCATCGCCGGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATAAGgcggcgcg                                                                                                                                            <  2:441626/203‑1 (MQ=255)                                                                                                  gCATTTCGTCGTCTTCCCACAGGGTGCCGTCGACCAGCAGGCAGTCGGCACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATAAGGCGGCGCGGCGCTg                                                                                                                                      <  1:195757/203‑1 (MQ=255)                                                                                                   cATTTCGTCGTCTTCCCACAGGGTGCCGTCGACCAGCAGGCAGTCGGCACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGt                                                                                                                                                                               >  2:352776/1‑161 (MQ=255)                                                                                                    aTTTCGTCGTCTTCCCACAGGGTGCCGTCGACCAGCAGGCAGTCGGCACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGc                                                                                                                                                                   >  1:34859/1‑172 (MQ=255)                                                                                                             tcTTCCCACAGGGTGCCGTCGACCAGCAGGCAGTCGGCACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTgg                                                                                                                                                            >  2:195753/1‑170 (MQ=255)                                                                                                                           gCCGTCGACCAGCAGGCAGTCGGCACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGGCGAAGCGGTGTGGCGAATAAGGCGGCGCGGCGCTGCGCAGGGGGAAGGGAGTGAACTTgg                                                                                                             >  1:58134/1‑202 (MQ=255)                                                                                                                              gTCGACCAGCAGGCAGTCGGCACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATAAGGcggcgcggcg                                                                                                                                         >  2:359553/1‑172 (MQ=255)                                                                                                                              cgcctcggcctgccggggcgttttcgttttttcgatccggtttctgcatagagagtggccatgtacatccaggttctcggctccGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATAAGGCGGCGCGGCGCTGCGCAgggg                                                                                                                              <  2:125422‑M1/99‑1 (MQ=255)                                                                                                                              cgcctcggcctgccggggcgttttcgttttttcgatccggtttctgcatagagagtggccatgtacatccaggttctcggctccGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATAAGGCGGCGCGGCGCTGCGCAgggg                                                                                                                              >  1:125426‑M1/85‑183 (MQ=255)                                                                                                                                     agcagGCAGTCGGCACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATAAGGCGGCGCGGCGCTGCGCAGGGGGAAGGGAggg                                                                                                                    >  2:448437/1‑184 (MQ=255)                                                                                                                                                ggCACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATAAGGCGGCGCGGCGCTGCGCAGGGGGAAGGGAGTGAACTTGAGGTTGGGGCAGGCGTCGATCACGAAg                                                                                   <  1:206771/208‑1 (MQ=255)                                                                                                                                                  cACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGc                                                                                                                                                                             <  1:117943/116‑1 (MQ=255)                                                                                                                                                   aCCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGagg                                                                                                                                                                    >  2:439809/1‑122 (MQ=255)                                                                                                                                                   aCCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATAAGGCGGCGCGGCGCTGCGCAGGGGGAAGGGAGTGAACTTGAGGTTGGGGCAGGCGTCGATCACGAAGCTGCCTTCCAGCTCGATACGGTTCCACTGCa                                                    <  1:338330/236‑1 (MQ=255)                                                                                                                                                          atcatcGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATAAGGCGGCGCGGCGCTGCGCAGGGGGAAGGGAGTGAACTTGAGGTTGGGGCAGGCGTCGATCACGAAGCTGCCt                                                                             >  2:446208/1‑204 (MQ=255)                                                                                                                                                                    gcagcTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATAAGGCGGCGCGGCGCTGCGCAGGGGGAAGGGAGTGAACTTGAGGTTGGGGCAGGCGTCGATCACGAAGCTGCCTTCCAGCTCGATACGGTTCCACTGCAGGCCACCGTTCCAGTg                                    >  1:453500/1‑235 (MQ=255)                                                                                                                                                                                          cAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATAAGGCGGCGCGGCGCTGCGCAGGGGGAAGGGAGTGAACTTGAGGTTGGGGCAGGCGTCGATCACGAAGCTGCCTGCCAg                                                                        >  1:483458/1‑177 (MQ=255)                                                                                                                                                                                           aGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATAAGGCGGCGCGGCGCTGCGCAGGGGGAAGGGAGTGCACTTGAGGTTGGGGCAGGCggc                                                                                             >  1:69070/1‑153 (MQ=255)                                                                                                                                                                                                     cGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATAAGGCGGCGCGGCGCTGCGCAGGGGGAAGGGAGTGAACTTGAGGTTGGGGCAGGCGTCGATCACGAAGCTGCCTTCCAGCTCGATACGGTTCCACTGCAGGCCACCGTTCCAGTGGCTGAGCATGTTGAACAGCGGGAAACCGGTGGTc  <  1:313749/236‑1 (MQ=255)                                                                                                                                                                                                                ttGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATAAGGCGGCGCGGCGCTGCGCAGGGGGAAGGGAGTGAACTTGAGGTTGGGGCAGGCGTCGATCACGAAGCTGCCTTc                                                                           <  2:509090/152‑1 (MQ=255)                                                                                                                                                                                                                  GCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCg                                                                                                                                                                       <  2:447264‑M1/58‑1 (MQ=255)                                                                                                                                                                                                                  GCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCg                                                                                                                                                                       >  1:447268‑M1/1‑58 (MQ=255)                                                                                                                                                                                                                  |                                                                                                                                                                                                                               CGCGGGAAGCCGTCGAGCACTTCCAGCATGCCGCCAGGGCCGTTCTGGGCCAGGTGACCCATCTCGCGGCCGGTGCGGGTGCCCACGCCACGGCGCTGCATTTCGTCGTCTTCCCACAGGGTGCCGTCGACCAGCAGGCAGTCGGCACCGTGCATCATCGCCAGCAGCTTTTCGTCTACCTGGCCCAGGCCCGGGGCGTAGAACAGCTTGCCGCCGGTGCGGGTGTCTTCGACCATCAAACCAAGGTTATCGCCCGGGTGCGGGTCGAAGCGGTGTGGCGAATAAGGCGGCGCGGCGCTGCGCAGGGGGAAGGGAGTGAACTTGAGGTTGGGGCAGGCGTCGATCACGAAGCTGCCTTCCAGCTCGATACGGTTCCACTGCAGGCCACCGTTCCAGTGGCTGAGCATGTTGAACAGCGGGAAACCGGTGGTC  >  AE015451/459070‑459501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.