New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 4945197	28 (1.090)	5 (0.200)	5/304	NT	20.9%	intergenic (+557/+241)	PP_5673/flhB	putative D‑alanine‑‑D‑alanine ligase/flagellin export apparatus, substrate specificity protein
	?	AE015451	4945389 =	11 (0.450)					intergenic (+749/+49)	PP_5673/flhB	putative D‑alanine‑‑D‑alanine ligase/flagellin export apparatus, substrate specificity protein

CATCACCAGCAGTAACAGCGGCACACCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGCGGCTGGCATTGCTAGAAGCTTTCCAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAG  >  AE015451/4944965‑4945362                                                                                                                                                                                                                                         |                                                                                                                                                                      cATCACCAGCAGTAACAGCGGCACACCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGCGGCTGGCATTGCTAGAAGCTTTCCAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGccgccc                                                                                                                                                                     <  1:501440/235‑1 (MQ=255)         gcagTAACAGCGGCACACCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGCGGCTGGCATTGCTAGAAGCTTTCCAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGgcgc                                                                                                                                                            <  2:419744/236‑1 (MQ=255)                    ggCACACCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGCGGCTGGCATTGCTAGAAGCTTTCCAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCaa                                                                                                                                                 <  1:219164/236‑1 (MQ=255)                                     gcGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGCGGCTGGCATTGCTAGAAGCTTTCCAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAgct                                                                                                                                              <  1:467175/221‑1 (MQ=255)                                      cGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGCGGCTGGCATTGCTAGAAGCTTTCCAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCgccgcc                                                                                                                                                                      >  2:339833/1‑197 (MQ=255)                                                    cAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGCGGCTGGCATTGCTAGAAGCTTTCCAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGtccc                                                                                                                 <  1:285687/236‑4 (MQ=255)                                                                      gCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGCGGCTGGCATTGCTAGAAGCTTTCCAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTAcgcg                                                                                               <  1:503056/236‑1 (MQ=255)                                                                         gATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGCGGCTGGCATTGCTAGAAGCTTTCCAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCgcgggcggcgctcgatctcacaggcaacgcaaaccta                                                                                                                                  <  1:180127‑M1/198‑38 (MQ=255)                                                                         gATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGCGGCTGGCATTGCTAGAAGCTTTCCAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCgcgggcggcgctcgatctcacaggcaacgcaaaccta                                                                                                                                  >  2:180123‑M1/1‑161 (MQ=255)                                                                                  aGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGCGGCTGGCATTGCTAGAAGCTTTCCAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAg                                                                                                                                                <  1:304870/175‑1 (MQ=255)                                                                                  aGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGCGGCTGGCATTGCTAGAAGCTTTCCAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAg                                                                                                                                                >  2:304866/1‑175 (MQ=255)                                                                                  aGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGCGGCTGGCATTGCTAGAAGCTTTCCAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTAcgcgc                                                                                              >  1:273250/1‑225 (MQ=255)                                                                                  aGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGCGGCTGGCATTGCTAGAAGCTTTCCAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTAcgcgc                                                                                              <  2:273246/225‑1 (MQ=255)                                                                                    tGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGCGGCTGGCATTGCTAGAAGCTTTCCAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTc                                                                                                                            <  1:123061/193‑1 (MQ=255)                                                                                    tGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGCGGCTGGCATTGCTAGAAGCTTTCCAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTc                                                                                                                            >  2:123057/1‑193 (MQ=255)                                                                                    tGCGAGCCCCCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGCGGCTGGCATTGCTAGAAGCTTTCCAACGTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTcgc                                                                                  <  1:54159/235‑1 (MQ=255)                                                                                       gATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGCGGCTGGCATTGCTAGAAGCTTTCCAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGGTCGAGCGCCGCCCGCGCGGCGCATCGCGAGCaa                                                                                                                                                 >  2:371451/1‑169 (MQ=255)                                                                                                  aGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGCGGCTGGCATTGCTAGAAGCTTTCCAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATAtt                                                                   <  1:336837/236‑1 (MQ=255)                                                                                                                    cTTTGACGCCCAGGGGGCGCCTGCGGCTGGCATTGCTAGAAGCTTTCCAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGccgcccg                                                                                                                                                                    >  2:271130/1‑121 (MQ=255)                                                                                                                          cGCCCAGGGGGCGCCTGCGGCTGGCATTGCTAGAAGCTTTCCAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCg                                                                                         <  1:496374/190‑1 (MQ=255)                                                                                                                          cGCCCAGGGGGCGCCTGCGGCTGGCATTGCTAGAAGCTTTCCAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCCCCGCgcgggcggcgctcgatctcacaggcaacgcaaacctatcgccgaacagatcaactgtcgcggcgcaggtcc                                                                                                >  1:133310‑M1/1‑112 (MQ=255)                                                                                                                              cAGGGGGCGCCTGCGGCTGGCATTGCTAGAAGCTTTCCAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTcgcgcgc                                       <  1:375904/236‑1 (MQ=255)                                                                                                                                   ggCGCCTGCGGCTGGCATTGCTAGAAGCTTTCCAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCgg                                  <  1:252914/236‑1 (MQ=255)                                                                                                                                       ccTGCGGCTGGCATTGCTAGAAGCTTTCCAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTAAACCTCGATAtt                                                                   <  1:276283/199‑1 (MQ=255)                                                                                                                                        cggCGGCTGGCATTGCTAGAAGCTTTCCAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTcgcgcgc                                       <  2:359406/224‑1 (MQ=255)                                                                                                                                             gCTGGCATTGCTAGAAGCTTTCCAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACaggagg                        <  1:339837/236‑1 (MQ=255)                                                                                                                                                         ggAAGCTTTCCAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTcgc                                                                                  <  2:316516/165‑1 (MQ=255)                                                                                                                                                         aGAAGCTTTCCAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGa             <  1:418345/235‑1 (MQ=255)                                                                                                                                                                   cAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCgcgggcggcgctcgatctcacaggcaacgcaaacctatcgccgaacagatcaactgtcgcggcgcaggtccggcgggatcggcaggtcgtccttcagtggctctgggcgcttgcccttgccggcgcggtattggcgaatctggaacacatacgccagcacctgcg  >  1:432002‑M1/1‑71 (MQ=255)                                                                                                                                                                            gATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCgcgggcggcgctcgatctcacaggcaacgcaaacctatcgccgaacagatcaactgtcgcggcgcaggtccggcgggatcggcaggtcgtccttcagtggctctgggcgcttgcccttgccggcgcg                                        >  2:167226‑M1/1‑62 (MQ=255)                                                                                                                                                                            gATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGcac                              <  1:371455/199‑1 (MQ=255)                                                                                                                                                                                                  tCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGccgccc                                                                                                                                                                     >  1:392638/1‑42 (MQ=255)                                                                                                                                                                                                  tCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGccgccc                                                                                                                                                                     <  2:392634/42‑1 (MQ=255)                                                                                                                                                                                                                ttGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAgct                                                                                                                                              <  2:63594/51‑1 (MQ=40)                                                                                                                                                                                                                          tgAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTAcgcgc                                                                                              >  2:176753/1‑89 (MQ=255)                                                                                                                                                                                                                          tgAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTAcgcgc                                                                                              <  1:176757/89‑1 (MQ=255)                                                                                                                                                                                                                              aTCGAGCGCCGCCCGCGCGGCGCAGCGCGGGCAAGCTCGCCCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATAtt                                                                   <  2:14536/112‑1 (MQ=255)                                                                                                                                                                                                                               tCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTcgcgcgtaaatc                                  <  2:248687/144‑7 (MQ=255)                                                                                                                                                                                                                                      ccgcccgcGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTcgcgc                                         <  2:157007/130‑1 (MQ=255)                                                                                                                                                                                                                                       cgcgcgggcggcgctcgatctcacaggcaacgcaaacctatcgccgaacagatcaactgtcgcggcgcaggtccggcgggatcggcaggtcgtccttcagtggctctgggcgcttgcccttgccg                                             <  2:46420‑M1/125‑123 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                      CATCACCAGCAGTAACAGCGGCACACCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGCGGCTGGCATTGCTAGAAGCTTTCCAACTTTGTGATCTGCTGTGAATCGGGGTGTTCGCCGTGAGGTTTTTGTCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAG  >  AE015451/4944965‑4945362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.