Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	AE015451	867,950	45 bp→TTCGGTA	9.9%	intergenic (+48/+85)	PP_0750 → / ← mqo‑I	conserved protein of unknown function/malate:quinone oxidoreductase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 867949	31 (1.200)	3 (0.120) +TTCGGTA	3/304	NT	9.9%	intergenic (+47/+130)	PP_0750/mqo‑I	conserved protein of unknown function/malate:quinone oxidoreductase
	?	AE015451	867995 =	26 (1.010)					intergenic (+93/+84)	PP_0750/mqo‑I	conserved protein of unknown function/malate:quinone oxidoreductase

TAGGCTTCTTCGTCGAAGCTGGCCTGGACCTGGACGGCAAGGACGAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTCCAGGGCCGCGTCGTTATCGGCACTGATCTTGTGGTACAGCGCAGCATCGGTTGCCAGGGTCTTCTCGCGAGCCGGGAAGATTTCCTTGAG  >  AE015451/867772‑868210                                                                                                                                                                                                                                |                                                                                                                                                                                                                        tAGGCTTCTTCGTCGAAGCTGGCCTGGACCTGGACGGCAAGGACGAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAaga                                                                                                                                                                                                             <  1:351442/236‑1 (MQ=255)      tccttcGTCGAAGCTGGCCTGGACCTGGCCGGCAAGGACGCGCACGGCAAGCCCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAag                                                                                                                                                                                                              <  1:49881/228‑1 (MQ=255)           cgtcgAAGCTGGCCTGGACCTGGACGGCAAGGACGAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCAt                                                                                                                                                                                                                        <  2:379043/215‑1 (MQ=255)                                         ggACGAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGGGGGAGCGGGCATGCCcgcg                                                                                                                                                                                                                 >  2:182485/1‑192 (MQ=255)                                                                                                        cGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGtt                                                                                                                                                        >  1:251704/1‑186 (MQ=255)                                                                                                          agagCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCg                                                                                                                                                                                                        <  1:506756/136‑1 (MQ=255)                                                                                                           gaTCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCATGCCcgc                                                                                                                                                                                                                  >  1:221762/1‑125 (MQ=255)                                                                                                           gaTCATCGATAAAGCCCGTGGCTCATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCATGCCcgc                                                                                                                                                                                                                  <  2:221758/125‑1 (MQ=255)                                                                                                                        gCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCATg                                                                                                                                                                                                                       <  1:89411/107‑1 (MQ=255)                                                                                                                            gTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTCCAGGGCcgc                                                                                   >  1:497448/1‑235 (MQ=255)                                                                                                                             tGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAgg                                                                                                                                                                                 <  2:383674/140‑1 (MQ=255)                                                                                                                              ggCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGcc                                                                                                                                                                    >  2:374936/1‑152 (MQ=255)                                                                                                                                                 gatcatcgataaagcccgtggctgatctgtagccagtgccgccctcttcgcgggcatgcccgctcccacaggttcggtaATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGcc                                                                                                                                                                                    >  2:469112‑M2/80‑117 (MQ=255)                                                                                                                                                 gatcatcgataaagcccgtggctgatctgtagccagtgccgccctcttcgcgggcatgcccgctcccacaggttcggtaATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGcc                                                                                                                                                                                    <  1:469116‑M2/38‑1 (MQ=255)                                                                                                                                                  cGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTCCCGAACCTGTGGGAGCGGGCATGCCCGCGAaga                                                                                                                                                                                                             <  1:64339/91‑1 (MQ=255)                                                                                                                                                  cGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAACAACGCCCCGAGGGGc                                                                                                                                                           >  2:198038/1‑141 (MQ=255)                                                                                                                                                         ttCGCGGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGcc                                                                                                                                                                    <  2:251700/125‑1 (MQ=255)                                                                                                                                                            gcgGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTCCAGGGCCGCGTCGTTATCGGCACTGATCTTGTGGTACAgc                                                    >  2:223026/1‑234 (MQ=255)                                                                                                                                                            gcgGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCG‑TTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGctctct                                                                                                    <  2:251267/185‑1 (MQ=255)                                                                                                                                                            gcgGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCG‑TTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTCCAGGGCCGCGTCGTTATCGGCACTGATCTTGTGGTa                                                        >  2:270908/1‑229 (MQ=255)                                                                                                                                                               ggCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGctctct                                                                                                    <  1:5211/183‑1 (MQ=255)                                                                                                                                                                   cgcCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTaa                                                                                                                                  <  1:81314/149‑1 (MQ=255)                                                                                                                                                                            ccACAGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTCCAGGGCCGCGTCGTTATCGGCACTGATCTTGTGGTACAGCGCAGCATCGGTTGCCAg                                   <  1:182489/235‑1 (MQ=255)                                                                                                                                                                             cacaGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTCCAGGGCCGCGTCGTTATCGGCACTGATCTTGTGGTACAGCGCAGCATCGGTTGCCAgg                                  >  1:290269/1‑235 (MQ=255)                                                                                                                                                                              acaGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGctctct                                                                                                    <  1:256542/168‑1 (MQ=255)                                                                                                                                                                                       gcgcCGCACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTCCAGGGCCGCGTCGTTATCg                                                                         >  2:468475/1‑186 (MQ=255)                                                                                                                                                                                             cACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGctctct                                                                                                    <  1:86210/153‑1 (MQ=255)                                                                                                                                                                                             cACCGGCGGGCATTAGCGAACCTGGGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAgg                                                                                                                                                              <  2:26192/95‑1 (MQ=255)                                                                                                                                                                                                    gggCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTCCAGGGCCGCGTCGTTATCGGCACTGATCTTGTGGTACAGCGCAGCATCGGTTGCCAGGGTCTTCTCGCGAGCCGGGCAGAtt          >  1:504302/1‑236 (MQ=255)                                                                                                                                                                                                            ccgctcccacaggttcggtaATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTCCAGGGCCGCGTCGTTATCGGCACTGATCTTGTGGTACAGCGCAGCATCGGTTGCCAGGGTCTTCTCGCGAGCCGGGAAGATTTCCTTGAg  >  1:452481‑M2/21‑236 (MQ=255)                                                                                                                                                                                                                    gtgGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTCCAGGGCCGCGTCGTTATCGGCACTGATCTTGTGGTa                                                        <  1:189980/174‑1 (MQ=255)                                                                                                                                                                                                                           ggggCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTgg                                                                                                                     <  2:19923/105‑1 (MQ=255)                                                                                                                                                                                                                                aTGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCgg                                                                                                              >  2:213818/1‑108 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                        TAGGCTTCTTCGTCGAAGCTGGCCTGGACCTGGACGGCAAGGACGAGCACGGCAAGACCTTCGTCGAGCAGATTGCCGACCAGCGCAATGCCCCTGAGTACATCGAGATCATCGATAAAGCCCGTGGCTGATCTGTAGCCAGTGCCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAGTCGCGCCGCACCTGCGGGCATTACCGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGGCGGTACAGGCTGATCAAGCGCCAGGCACAAAAAACGCCCCGAGGGGCGTTTTTGCGTTTCAGACCGGCTTAAGCGTAGTGCTTGGCCGCCGGGCTGCTCTCTGCCAGGTCCAGGGCCGCGTCGTTATCGGCACTGATCTTGTGGTACAGCGCAGCATCGGTTGCCAGGGTCTTCTCGCGAGCCGGGAAGATTTCCTTGAG  >  AE015451/867772‑868210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.