Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	AE015451	1,780,067	Δ62 bp	13.6%	intergenic (+26/+19)	PP_1587 → / ← dapC	NhaP‑type Na+/H+ and K+/H+ antiporters/N‑succinyl‑L,L‑diaminopimelate aminotransferase alternative

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 1780066	25 (0.970)	4 (0.160)	4/304	NT	13.6%	intergenic (+25/+81)	PP_1587/dapC	NhaP‑type Na+/H+ and K+/H+ antiporters/N‑succinyl‑L,L‑diaminopimelate aminotransferase alternative
	?	AE015451	1780129 =	27 (1.100)					intergenic (+88/+18)	PP_1587/dapC	NhaP‑type Na+/H+ and K+/H+ antiporters/N‑succinyl‑L,L‑diaminopimelate aminotransferase alternative

CGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGCACCCGGGTTCACCCCATCCACTTCACGCGACAGGTACGACCCCGGTACCACGGTTACATGCTGGGCTTCGAACAGGTCGCGGGTGAACTCGGCATCGTCGCCCGGTACCTTGGCCCACAGGTA  >  AE015451/1779931‑1780359                                                                                                                                                                                                       |                                                                                                                                                                                                                                       cGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAg                                                                                                                                                                                                    >  1:302067/1‑235 (MQ=255)                          gTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGtat                                                                                                                                                                                                             >  1:111420/1‑199 (MQ=255)                                    ttacgcttgcgtgccctgagggcgcagcggttggagctgtacaacctgcatcgtcagcacctggtgggggatgaggtggtgcggcaagtactgggtgagctggatatgagtgaggcgaacctgggttcagtcaagtagaccctatcaaggcccttcgcgggcgTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCg                                                                                                                                                                   >  2:432946‑M2/164‑233 (MQ=255)                                        ggggATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTGGATCAGCGGTTTTGCAGGACGGCGCGaacg                                                                                                                                                                                           >  1:207154/1‑203 (MQ=255)                                               aGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGtt                                                                                                                                                                                                              >  1:205557/1‑179 (MQ=255)                                                  tggtgCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCa                                                                                                                                                   >  1:179200/1‑235 (MQ=255)                                                        ggCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGc                                                                                                                                               >  2:291984/1‑233 (MQ=255)                                                             gTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATgc                                                                                                                                          <  2:302063/233‑1 (MQ=255)                                                                       aGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCaa                                                                                                                                                      >  1:293113/1‑211 (MQ=255)                                                                       aGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGc                                                                                                                             <  1:291988/236‑1 (MQ=255)                                                                                         cGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGCACCCGGGGTCACCCCATc                                                                                                           >  1:280175/1‑236 (MQ=255)                                                                                             cacctggtgggggatgaggtggtgcggcaagtactgggtgagctggatatgagtgaggcgaacctgggttcagtcaagtagaccctatcaaggcccttcgcgggcgTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCgg                                                                                                                                                                  <  1:48307‑M2/71‑1 (MQ=255)                                                                                                       gTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGc                                                                                                                                                        <  2:123820/177‑1 (MQ=255)                                                                                                        gaaaGTAGACCCTATCAAGGCCCTTCGGGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCGGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCt                                                                                                                                                                                       <  1:76073/143‑1 (MQ=255)                                                                                                                   cTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGTGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCTGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCAcg                                                                                                                                     >  1:101108/1‑184 (MQ=255)                                                                                                                       cAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTccg                                                                                                                                                                                    >  1:512545/1‑133 (MQ=255)                                                                                                                         aGGCCCTTCGCGGGCGCGCCCGGTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTCGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCtt                                                                                                                                                                              <  1:349288/137‑1 (MQ=255)                                                                                                                         aGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGGGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGgcgc                                                                                                                                                                                                >  1:411840/1‑119 (MQ=255)                                                                                                                                                  tgaggcgaacctgggttcagtcaagtagaccctatcaaggcccttcgcgggcgTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGCACCCGGGTTCACCCCATCCACTTCACGCGACAGGTACGACCCCGGTACCACGGTTACATGCTGGGCTTCGAACa                                                   <  1:437371‑M2/182‑1 (MQ=255)                                                                                                                                                      aGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGGGGGAGCGGGAGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGCACCCGGGTTCACCCCATCCACTTCACGCGACAGGTACGACCCCGGTa                                                                              <  1:186273/204‑1 (MQ=255)                                                                                                                                                       cgaacctgggttcagtcaagtagaccctatcaaggcccttcgcgggcgTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGCACCCGGGTTCACCCCATCCACTTCACGCGACAGGTACGACCCCGGTACCACGGTTACATGCTGGGCTTCGAACAGGTcgc                                             >  1:436583‑M2/49‑236 (MQ=255)                                                                                                                                                               gctgcgctTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGCACCCGGGTTCACCCCATCCACTTCACGCGACAGGTACGACCCCGGTACCACGGTTACATGCTGGGc                                                           >  2:472619/1‑214 (MQ=255)                                                                                                                                                               gctgcgctTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGCACCCGGGTTCACCCCATCCACTTCACGCGACAGGTACGACCCCGGTACCACGGTTACATGCTGGGCTTCGAACAGGTCGCGGGTGaa                                      >  1:475320/1‑235 (MQ=255)                                                                                                                                                                 tgcgctTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGCACCCGGGTTCACCCCATCCACTTCACGCGACAg                                                                                            >  2:213615/1‑179 (MQ=255)                                                                                                                                                                     ctTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTc                                                                                                                                                                    <  1:182357/103‑1 (MQ=255)                                                                                                                                                                     ctTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTc                                                                                                                                                                    >  2:182353/1‑103 (MQ=255)                                                                                                                                                                      ttGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGCACCCGGGTTCACCCCATCCACTTCACGCGACAGGTACGACCCCGGTACCACGGTTACATGCTGGGCTTCGAACAGGTCGCGGGt                                         >  1:194738/1‑225 (MQ=255)                                                                                                                                                                      ttGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTACGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGCACCCGGGTTCACCCCATCCACTTCACGCGACAGGTACGACCCCGGTACCACGGTTACATg                                                                 >  1:224552/1‑201 (MQ=255)                                                                                                                                                                           aTCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGCACCCGGGTTCACCCCATCCACTTCACGCGACAGGTAc                                                                                        >  2:328745/1‑173 (MQ=255)                                                                                                                                                                                   tGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGCACCCGGGTTCACCCCATCCACTTCACGCGACAGGTACGACCCCGGTACCACg                                                                         >  2:452736/1‑180 (MQ=255)                                                                                                                                                                                   tGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGCACCCGGGTTCACCCCATCCACTTCACGCGACAGGTACGACCCCGGTACCACg                                                                         <  1:452740/180‑1 (MQ=255)                                                                                                                                                                                           tgGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGCACCCGGGTTCACCCCATCCACTTCACGCGACAGGTACGACCCCGGTACCACGGTTACATGCTGGGCTTCGAACAGGTCGCGGGTGAACTCGGCa                               <  2:179196/214‑1 (MQ=255)                                                                                                                                                                                             ggAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGCACCCGGGTTCACCCCATCCACTTCACGCGACAGGTACGACCCCGGTACCACGGTTACATGCTGGGCTTCGAACAGGTCGCGGGTg                                        >  2:152325/1‑203 (MQ=255)                                                                                                                                                                                                 cgggcgTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGAAGCATTCGGCCAGCGGTGCAACCAGTGCCAGGCGCACGCGCCCGGCACCCGGGTTCACCCCATCCAc                                                                                                        >  2:27237‑M2/7‑135 (MQ=255)                                                                                                                                                                                                  gggcgTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCcgccgc                                                                                                                                                                                >  2:48081‑M2/6‑62 (MQ=255)                                                                                                                                                                                                  gggcgTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGCACCCGGGTTCACCCCATCCACTTCACGCGACAGGTACGACCCCGGTACCACGGTTACATGCTGGGCTTCGAACAGGTCGCGGGTGAACTCGGCATCGTCTCCCGGTACCTTGGCCCACAGGTa  >  1:168824‑M2/6‑236 (MQ=255)                                                                                                                                                                                                    gcgTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCGCCGCCGCTTCGATGCATTCGGCCAGCg                                                                                                                                                            >  2:51027‑M2/4‑80 (MQ=255)                                                                                                                                                                                                       |                                                                                                                                                                                                                                       CGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGCACCCGGGTTCACCCCATCCACTTCACGCGACAGGTACGACCCCGGTACCACGGTTACATGCTGGGCTTCGAACAGGTCGCGGGTGAACTCGGCATCGTCGCCCGGTACCTTGGCCCACAGGTA  >  AE015451/1779931‑1780359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.