| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 199,915 | T→C | 18.0% | F1807F (TTT→TTC) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 199,915 | 0 | T | C | 18.0% | 108.2 / 8.6 | 43 | F1807F (TTT→TTC) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base T (21/14); new base C (2/6); total (23/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
GATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAAC > AE015451/199696‑200142 | gaTCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGttt > 1:446791/1‑220 (MQ=38) cccTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGc > 1:42305/1‑236 (MQ=40) ccTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTgg > 2:421974/1‑218 (MQ=255) ccAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGcccc > 1:421116/1‑233 (MQ=255) gTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACcgacga > 1:438619/1‑235 (MQ=255) aCTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAg < 2:250564/188‑1 (MQ=255) ggAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAg > 1:203820/1‑202 (MQ=25) aCTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACAt > 1:251488/1‑235 (MQ=255) ttCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGAc > 2:411826/1‑171 (MQ=255) gCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTGACCGACGAGCCAGGc < 2:273173/205‑1 (MQ=14) gCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTGACCGACGAGCCAGGc > 1:273177/1‑205 (MQ=21) aCGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGAc > 1:402031/1‑218 (MQ=255) ccacTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAg < 2:471762/221‑1 (MQ=255) cTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTGACCGACGAGcc < 2:282316/184‑1 (MQ=2) cTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTGACCGACGAGcc > 1:282320/1‑184 (MQ=255) tGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGcc < 2:134719/183‑1 (MQ=255) ggCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACg < 1:380340/220‑1 (MQ=255) tcAGCCCCCCGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGc < 2:89064/145‑1 (MQ=255) aGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGc > 2:240301/1‑163 (MQ=255) aGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGc < 1:240305/163‑1 (MQ=255) accacGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTCGAAaacctga < 2:150409/118‑1 (MQ=9) accacGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTCGAAaacctga > 1:150413/1‑118 (MQ=255) cGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCaaa > 1:489785/1‑236 (MQ=255) gCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGaaa > 2:139321/1‑105 (MQ=255) gCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTg > 1:468043/1‑201 (MQ=255) aaCGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCa > 2:436693/1‑233 (MQ=255) gTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAg < 2:243813/159‑1 (MQ=14) tCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTg < 1:436697/236‑1 (MQ=255) cTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTgg > 1:256618/1‑86 (MQ=38) tATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTgg < 1:216949/235‑1 (MQ=255) tATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGGTTGAAAACCTGAACATGGAAAAGACCCCg > 2:214025/1‑96 (MQ=255) gCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGa < 1:421978/235‑1 (MQ=255) aCCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGa < 2:446787/233‑1 (MQ=255) aCCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCaa > 2:433213/1‑215 (MQ=255) ccAACCAGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACcgac < 1:255652/107‑1 (MQ=12) aCGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCa < 2:42302/163‑1 (MQ=40) ccGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTc < 1:358100/180‑1 (MQ=25) aaTGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTc > 2:189478/1‑171 (MQ=38) aaTGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTc < 1:189482/171‑1 (MQ=38) cATCGACGCGGTCAGGGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCTGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAAt < 1:483092/235‑1 (MQ=255) gTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGCACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGACGGCCACATCGTCa > 2:101254/1‑112 (MQ=255) gcggcgCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGcc < 2:417637/184‑1 (MQ=14) aTGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACACCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACCGCCGACCAGGCTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGAc > 1:289786/1‑188 (MQ=17) aaGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAAc > 2:324811/1‑233 (MQ=255) | GATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAAC > AE015451/199696‑200142 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |