| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 499,203 | A→G | 100% | intergenic (+47/‑249) | uhpA → / → PP_0411 | DNA‑binding response regulator UhpA/Polyamine ABC transporter, ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 499,203 | 0 | A | G | 100.0% | 85.6 / NA | 24 | intergenic (+47/‑249) | uhpA/PP_0411 | DNA‑binding response regulator UhpA/Polyamine ABC transporter, ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (7/17); total (7/17) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCGGAGCGGGAATTTTCGGTGTTCGAGCGCCTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCCATCCGGATGATGGCATTCTCTCAGTACCCCCGATTTTCGGGCGCT > AE015451/498974‑499398 | tcGGAGCGGGAATTTTCGGTGTTCGAGCGCCTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATg < 1:133885/236‑1 (MQ=255)tcGGAGCGGGAATTTTCGGTGTTCGAGCGCCTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATg > 1:500987/1‑236 (MQ=255) cGGAGCGGGAATTTTCGGTGTTCGAGCGCCTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGc < 1:176110/236‑1 (MQ=255) tgtTCGAGCGCCTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggcctggc > 1:142400/1‑235 (MQ=255) cgcCTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGgcctggcctg < 2:306625/230‑1 (MQ=255) aCGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGacc > 2:507501/1‑197 (MQ=255) cATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGc < 1:424796/236‑1 (MQ=255) gCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCctct < 1:57351/224‑1 (MQ=255) ttGGCGCTGTCGAGCAAGACCATCAGCACCCACTAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggcct < 1:287343/182‑1 (MQ=255) cATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCt < 1:183860/234‑1 (MQ=255) cTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTc < 2:142396/191‑1 (MQ=255) gCTGGCGGAGCTGTTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGc < 1:30030/159‑1 (MQ=255) gAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGgcctggcctg < 2:342068/105‑1 (MQ=255) aTGGCGCACAAGCTGATGTGGTTGCGGCATACGGGTGCCCGAGACTTGCCGAAGCCTATGTGGGCGCGTGCATGCCCGCGACCGGgcctggcctg < 2:114569/95‑1 (MQ=255) cGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCAt < 2:56147/49‑1 (MQ=255) aTACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCCATCCGGATGATGGCATTCTCTCAGTACCCCCGATTTTCGGGCGCt > 1:380297/1‑235 (MQ=255) ggTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCtt > 1:463548/1‑183 (MQ=255) cccGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCctct < 2:239481/85‑1 (MQ=255) cccGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCctct > 1:239485/1‑85 (MQ=255) cGAAACTTGCCGAAGCCTATGTGGGGGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCCATCCGGATGATGGCATTCTCTCAGTACCCCCGATTTTCGGGCGCt < 2:380293/224‑1 (MQ=255) aaCTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc > 1:515857/1‑37 (MQ=255) aaCTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc < 2:515853/37‑1 (MQ=255) gtGCCGAAGCCTTTGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACaaa < 2:462783/166‑1 (MQ=255) gtgGGAGCGGGCATGCCCGGGGACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCGAACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCCATCCGgatgat < 1:455111/170‑1 (MQ=255) | TCGGAGCGGGAATTTTCGGTGTTCGAGCGCCTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCCATCCGGATGATGGCATTCTCTCAGTACCCCCGATTTTCGGGCGCT > AE015451/498974‑499398 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |