| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 3,212,278 | A→T | 27.8% | *189R (TGA→AGA) ‡ | PP_2816 ← | putative Transcriptional regulator NfxB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 3,212,278 | 0 | A | T | 27.8% | 29.4 / 9.0 | 18 | *189R (TGA→AGA) ‡ | PP_2816 | putative Transcriptional regulator NfxB |
| Reads supporting (aligned to +/- strand): ref base A (6/7); new base T (2/3); total (8/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
ATCGCAATACCTTGCTGCGCCGGCTGGAGCGCGCCCAGGACCTGCTGCCTATCCCGCTTGCCGACCATCGCATCCAGATCGCCGCTGCCCTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGACCATGCCATGCAACAGCGATACGAACATCTCGGTCAGCAACGCCGCGGTGACATCCGCTCGAAATACGCCTTGTCGCTGGCCGCTGGCAAACAACGCATCGAGCCTTTCCAGATAAAACCGCAGGTCGTGCT > AE015451/3212081‑3212502 | aTCGCAATACCTTGCTGCGCCGGCTGGAGCGCGCCCAGGACCTGCTGCCTATCCCGCTTGCCGACCATCGCATCCAGATCGCCGCTGCCCTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCcgcg > 2:509700/1‑212 (MQ=255) cgcCGGCTGGAGCGCGCCCAGGACCTGCTGCCTATCCCGCTTGCCGACCATCGCATCCAGATCGCCGCTGCCCTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGc < 1:509704/235‑1 (MQ=255) cAGGACCTGCTGCCTATCCCGCTTGCCGACCATCGCATCCAGATCGCCGCTGCCCTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGc > 1:400069/1‑186 (MQ=255) gCTTGCCGACCATCGCATCCAGATCGCCGCTGCCCTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGTCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTCcac > 2:208167/1‑199 (MQ=255) gCATCCAGATCGCCGCTGCCCTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGc < 2:148736/183‑1 (MQ=255) gccgcTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGACCATGCCATGCAACAGCGATACGAACATCTCGGTCAGCa < 1:73073/217‑1 (MQ=255) aTAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGACCATGCCATGCAACAGCGATACGAACATCTCGGTCAGCAACGCCGCGGTGACATCCGCTCGAAATACGCCTTGTcgct < 2:480142/235‑1 (MQ=255) tAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTACGCGGGCGCCCTATTGAGCGCCCGCGTGGCAATGCCCTCTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTggcg < 2:288102/148‑1 (MQ=255) gCGGTCATTCCCGTCCTTTGACGCAAGAGGTGTTCACGGCTGTTGCCATGCGCGGGCGCCCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAAc < 2:66471/96‑1 (MQ=255) aTTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGACCATGCCATGCAACAGCGATACGAACATCTCGGTCAGCAACGCCGCgg > 2:331435/1‑196 (MQ=255) aTTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCCCTATTGAGCGCCCGCGTGGCAATGCCCTGTAGaa > 1:211326/1‑88 (MQ=38) aTTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCCCTATTGAGCGCCCGCGTGGCAATGCCCTGTAGaa < 2:211322/88‑1 (MQ=38) ggTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGACCATGCCATGCAACAGCGATACGAACATCTCGGTCAGCAACGCCGCGGTGACATCCGCTCGAAATACGCCTTGTcgctggccg > 2:131126/1‑209 (MQ=255) ggCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGACCCCGTTGGCGGGCGTCGACCATGCCATGCAACAGCGaga > 1:26872/1‑136 (MQ=255) ggCTGTTGCCCTGCGCGGGCGCCCTATTGAGCGCCCGCGTGGCAATGCCCTGTAGAAAc < 2:278638/59‑1 (MQ=21) ggCTGTTGCCCTGCGCGGGCGCCCTATTGAGCGCCCGCGTGGCAATGCCCTGTAGAAAc > 1:278642/1‑59 (MQ=25) gcaggCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGACCATGCCATGCAACAGCGATACGAACATCTCGGTCAGCAACGCCGCGGTGACATCCGCTCGAAATACGCCTTGTCGCTGGCCGCTGGCAAACAACGCATCGAGCCTTTCCAGATAAAACCGCAGGTCGtgct < 1:152152/232‑1 (MQ=255) tCAATAGGGCGCCCGCGTGGCAATGCGCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCGCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGa < 2:7366/95‑1 (MQ=255) | ATCGCAATACCTTGCTGCGCCGGCTGGAGCGCGCCCAGGACCTGCTGCCTATCCCGCTTGCCGACCATCGCATCCAGATCGCCGCTGCCCTTGAGCTGGTGATCTGGAGCACGCCGCTCGATGACGCCGAGCAATAGCGTTCATTCCCTTCCTTTGACGCAATAGGTCTTCACGGCTGTTGCCCTGCGCGGGCGCTCAATAGGGCGCCCGCGTGGCAATGCCCTGTAGAAACGCGCTCTCCAGCACCGTTGCACACTCTCTTGGCGCGCGCCCCCGTTGGCGGGCGTCGACCATGCCATGCAACAGCGATACGAACATCTCGGTCAGCAACGCCGCGGTGACATCCGCTCGAAATACGCCTTGTCGCTGGCCGCTGGCAAACAACGCATCGAGCCTTTCCAGATAAAACCGCAGGTCGTGCT > AE015451/3212081‑3212502 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |