| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 3,692,619 | T→A | 23.5% | Q336L (CAG→CTG) | PP_3259 ← | Acyl‑CoA dehydrogenase‑related protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 3,692,619 | 0 | T | A | 23.5% | 27.9 / 7.9 | 17 | Q336L (CAG→CTG) | PP_3259 | Acyl‑CoA dehydrogenase‑related protein |
| Reads supporting (aligned to +/- strand): ref base T (6/7); new base A (2/2); total (8/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.92e-01 | |||||||||||
GGCGCCCCGGCGGGCACAGTTGGCGTGAGTCGAGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTGGCCCCCAAGGCACGTCCGACGTGGCTTATGACCTGCTCTACGGCCTGTTCTACCTGGGCGCGGGTGCGGCGAACTTCGAA > AE015451/3692468‑3692803 | ggCGCCCCGGCGGGCACAGTTGGCGTGAGTCGAGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCaa > 1:414422/1‑230 (MQ=255) gcCCCGGCGGGCACAGTTGGCGTGAGTCGAGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCg < 2:349866/178‑1 (MQ=255) cccGGCGGGCACAGTTGGCGTGAGTCGAGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCAGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGActgctg > 1:208058/1‑236 (MQ=255) ttGGCGTGAGTCGAGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGc < 1:499789/234‑1 (MQ=255) cGAGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTGGCCCCCAAgg < 1:73459/236‑1 (MQ=255) aGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGTTCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCggg > 1:59142/1‑183 (MQ=255) aCGCGCCAGGTGCGCGCTTTGCTGCCCGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCa < 1:381856/154‑1 (MQ=255) gTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCg < 1:462926/202‑1 (MQ=255) tGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTAGGCGCtaa > 2:218420/1‑146 (MQ=255) cAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGGCGGGCAAAAGGACTGCTGCGACAg > 1:110660/1‑170 (MQ=255) gATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCCGGTCGGCGCTCAGCCGGGCa > 2:309947/1‑129 (MQ=255) tCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTGGCCCCCAAGGCACGTCCGACGTGGCTTATGACCTGCTCTACGGCCTGTTCTACCTGGGCGCGGGTGCGGCGAACTTCGaa < 1:262986/228‑1 (MQ=255) cTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCAGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGc < 1:372366/75‑1 (MQ=255) cTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCAGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGc > 2:372362/1‑75 (MQ=255) cTCATAGTTGCCACACCCCGGCTGGCAACTGAGCCAgctg > 2:61884/1‑40 (MQ=255) gTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTGGCCCCCAAGGCACGTCCGACGTGGCTTa > 1:350577/1‑165 (MQ=255) gTTGCCACACCCCGGCTGGCAACTGGGCCAGCAGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTGGCCCCCAAGGCACGTCCGACGTGGCt < 2:208054/163‑1 (MQ=255) cgCCGCACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTGGCCCCCAAGGCACGTCCGa < 1:108874/153‑1 (MQ=255) | GGCGCCCCGGCGGGCACAGTTGGCGTGAGTCGAGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTGGCCCCCAAGGCACGTCCGACGTGGCTTATGACCTGCTCTACGGCCTGTTCTACCTGGGCGCGGGTGCGGCGAACTTCGAA > AE015451/3692468‑3692803 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |