Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	AE015451	1,195,377	30 bp→TCGACCCGCTGCCACAG	9.3%	coding (1516‑1545/1653 nt)	uxpA →	Lipoprotein UxpA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 1195376	56 (0.970)	5 (0.100) +TCGACCCGCTGCCACAG	5/308	NT	9.3%	coding (1515/1653 nt)	uxpA	Lipoprotein UxpA
	?	AE015451	1195407 =	52 (0.900)					coding (1546/1653 nt)	uxpA	Lipoprotein UxpA

GCAGGGCCAGGCCTGGAGCGTCAGCTACAGCCCGCAAGGCAAGCGCATCACGGGGTTGGAGCCGGTGCAGGGCCTGTGCCGCACCTTTGGCCTGCAGTTCGAAAGCCAGGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/1195265‑1195376 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGGCTCGCCCGCCAGCCTGAGGATTGGCAACTGGCGCCGCTGCAACTGCCCGAAGTGCGTTACGTGCAGGGGCACCCAGGCTGGCACCCACGGCAATTGGCCTCGT  >  AE015451/1195407‑1195512                                                                                                                 |||||||||||||||||                                                                                                           GCAGGGCCAGGCCTGGAGCGTCAGCTACAGCCCGCAAGGCAAGCGCATCACGGGGTTGGAGCCGGTGCAGGGCCTGTGCCGCACCTTTGGCCTGCAGTTCGAAAGCCAGGCCTCGACCCGCTGCCACAGTGGCTCGCCCGCCAGCCTGAGGATTGGCAACTGGCGCCGCTGCAACTGCCCGAAGTGCGTTACGTGCAGGGGCACCCAGGCTGGCACCCACGGCAATTGGCCTCGT  >  1:460518/1‑235                                                   CGGGGTTGGAGCCGGTGCAGGGCCTGTGCCGCACCTTTGGCCTGCAGTTCGAAAGCCAGGCCTCGACCCGCTGCCACAGTGGCTCGCCCGCCAGCCTGAGG                                                                                      >  2:183563/1‑101                                                                               CCGCACCTTTGGCCTGCAGTTCGAAAGCCAGGCCTCGACCCGCTGCCACAGTGGCTCGCCTGCCAGCCTGAGGATTGGCAACTGGCGCCGCTGCAACTGCCCGAAGTGC                                                  <  1:316269/109‑1                                                                               CCGCACCTTTGGCCTGCAGTTCGAAAGCCAGGCCTCGACCCGCTGCCACAGTGGCTCGCCCGCCAGCCTGAGGATTGGCAACTGGCGCCGCTGCAACTGCCCGAAGTGC                                                  >  2:316266/1‑109                                                                                                        AGCGAGGCGTGGACCCGCTGCCACAGTGGCGCGCCCGCCAGCCTGAGGATTGGCAACTGGCGCCGCTGCAACTGCCCGAAGT                                                    <  2:357208/82‑1                                                                                                                    ACCCGCTGCCACAGTGGCGCGCCCGCCAGCCTGAGGATTGGCAACT                                                                            <  2:815358/46‑1                                                                                                                 |||||||||||||||||                                                                                                           GCAGGGCCAGGCCTGGAGCGTCAGCTACAGCCCGCAAGGCAAGCGCATCACGGGGTTGGAGCCGGTGCAGGGCCTGTGCCGCACCTTTGGCCTGCAGTTCGAAAGCCAGGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/1195265‑1195376 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGGCTCGCCCGCCAGCCTGAGGATTGGCAACTGGCGCCGCTGCAACTGCCCGAAGTGCGTTACGTGCAGGGGCACCCAGGCTGGCACCCACGGCAATTGGCCTCGT  >  AE015451/1195407‑1195512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.