Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	AE015451	1,780,067	Δ62 bp	17.4%	intergenic (+26/+19)	PP_1587 → / ← dapC	NhaP‑type Na+/H+ and K+/H+ antiporters/N‑succinyl‑L,L‑diaminopimelate aminotransferase alternative

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 1780066	55 (0.950)	11 (0.200)	11/328	NT	17.4%	intergenic (+25/+81)	PP_1587/dapC	NhaP‑type Na+/H+ and K+/H+ antiporters/N‑succinyl‑L,L‑diaminopimelate aminotransferase alternative
	?	AE015451	1780129 =	52 (0.940)					intergenic (+88/+18)	PP_1587/dapC	NhaP‑type Na+/H+ and K+/H+ antiporters/N‑succinyl‑L,L‑diaminopimelate aminotransferase alternative

CTGGAAAGCTACGACGACAGTGCTGAAGCCAAAGCCCTGGCCGCACAGATGGACTTGCTGGAGCGGCGTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGCACCCGGGTTCACCCCATCCACTTCACGCGACAGGTACGACCCCGGTACCACGGTTACATGCTG  >  AE015451/1779835‑1780299                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          cTGGAAAGCTACGACGACAGTGCTGAAGCCAAAGCCCTGGCCGCACAGATGGACTTGCTGGAGCGGCGTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGgcgcg                                                                                                                                                                                                                                         <  2:1022794/234‑1 (MQ=255)   ggAAAGCTACGACGACAGTGCTGAAGCCAAAGCCCTGGCCGCACAGATGGACTTGCTGGAGCGGCGTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGtgcccg                                                                                                                                                                                                                                     >  1:293321‑M1/1‑230 (MQ=255)                                 aGCCCTGGCCGCACAGATGGACTTGCTGGAGCGGCGTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAAt                                                                                                                                                                                                       <  1:943391/236‑1 (MQ=255)                                    ccTGGCCGCACAGATGGACTTGCTGGAGCGGCGTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAg                                                                                                                                                                                                    <  1:994030/236‑1 (MQ=255)                                     cTGGCCGCACAGATGGACTTGCTGGAGCGGCGTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAgc                                                                                                                                                                                                   <  1:761614/236‑1 (MQ=255)                                      tGGCCGCACAGATGGACTTGCTGGAGCGGCGTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAgc                                                                                                                                                                                                   <  2:500312/235‑1 (MQ=255)                                         ccGCACAGATGGACTTGCTGGAGCGGCGTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGtgcccgcgaaggggttgatcagcggttttgcaggaaggcgcgaa                                                                                                                                                                                               >  1:839457‑M1/1‑192 (MQ=255)                                            cacaGATGGACTTGCTGGAGCGGCGTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGccc                                                                                                                                                                                                                                      <  2:985574/194‑1 (MQ=255)                                             acaGATGGACTTGCTGGAGCGGCGTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCa                                                                                                                                                                                                     <  2:747141/226‑1 (MQ=255)                                              caGATGGACTTGCTGGAGCGGCGTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTcc                                                                                                                                                                                                                                 <  2:665528/197‑1 (MQ=255)                                               aGATGGACGTGCTGGAGCGGCGTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGGACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTActg                                                                                                                                                                                                                      <  2:115033/207‑1 (MQ=255)                                                gATGGACTTGCTGGAGCGGCGTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCa                                                                                                                                                                                                     >  2:1058364/1‑223 (MQ=255)                                                gATGGACTTGCTGGAGCGGCGTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCa                                                                                                                                                                                                     <  1:1058367/223‑1 (MQ=255)                                                       ttGCTGGAGCGGCGTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTActgcg                                                                                                                                                                                                                    <  2:600209/201‑1 (MQ=255)                                                       ttGCTGGAGCGGCGTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGa                                                                                                                                                                                                          <  1:218677/211‑1 (MQ=255)                                                          cTGGAGCGGCGTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAgc                                                                                                                                                                                                   <  2:693754/215‑1 (MQ=255)                                                                  gcgTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGa                                                                                                                                                                                                          >  1:771228/1‑200 (MQ=255)                                                                  gcgTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGCGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCcg                                                                                                                                                                       <  1:637796/235‑1 (MQ=255)                                                                        ccGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTActgcg                                                                                                                                                                                                                    <  2:304217/183‑1 (MQ=255)                                                                                 tGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAgc                                                                                                                                                                                                   <  2:906631/192‑1 (MQ=255)                                                                                  gCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAg                                                                                                                                                                                                                            >  2:651064/1‑166 (MQ=255)                                                                                  gCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAg                                                                                                                                                                                                                            <  1:651067/166‑1 (MQ=255)                                                                                  gCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTACCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTGCCt                                                                                                                                                                                          >  1:344011/1‑200 (MQ=255)                                                                                    ccTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAgcg                                                                                                                                                                                                  <  2:885843/190‑1 (MQ=255)                                                                                    ccTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCcg                                                                                                                                                                       <  1:1036356/217‑1 (MQ=255)                                                                                           gcgcAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTg                                                                                                                                                                           >  2:718010/1‑206 (MQ=255)                                                                                           gcgcAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTg                                                                                                                                                                           <  1:718013/206‑1 (MQ=255)                                                                                               aGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGGACTGCGCTGCGCTTGaaa                                                                                                                                                                                                        >  2:804042/1‑173 (MQ=255)                                                                                                               cAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGtgcccgcgaaggggttgatcagcggttttgcaggaaggcgcgaatgcgctccgccgcttcgatgcattcggccagcggtgcaaccagtgccatgcgcacgcgcccggcacccg                                                                                                                          <  1:755234‑M1/235‑114 (MQ=255)                                                                                                                aaCCTGCATCGTCAGCGCCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCcg                                                                                                                                                                       <  1:819727/189‑1 (MQ=255)                                                                                                                     gCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGCAGgcg                                                                                                                                     >  1:1073591/1‑218 (MQ=255)                                                                                                                          gTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTa                                                                                                                                                                                             <  2:510060/157‑1 (MQ=255)                                                                                                                          gTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGcgcg                                                                                                                                   >  1:326104/1‑215 (MQ=255)                                                                                                                          gTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGa                                                                                                               >  1:825479/1‑235 (MQ=255)                                                                                                                             aGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGACCCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGtgcccgcgaaggggttgatcagcggttttgcaggaaggcgcgaatgcgctccgccgcttcgatgcattcggccagcggtgcaaccagtgccatgcgcacgcgcccgg                                                                                                                                <  1:264964‑M1/215‑108 (MQ=255)                                                                                                                               cACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTa                                                                                                                                                                                                                         >  2:581397/1‑124 (MQ=255)                                                                                                                                aCCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTActgcgctg                                                                                                                                                                                                                 >  2:472820/1‑131 (MQ=255)                                                                                                                                aCCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTActgcgc                                                                                                                                                                                                                   >  1:867438/1‑129 (MQ=255)                                                                                                                                       gggggATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTGTGCAGGAAGGCGCGAATGCGCTccgc                                                                                                                       >  2:492288/1‑214 (MQ=255)                                                                                                                                        ggggATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATc                                                                                                                                                                                                      >  2:925486/1‑134 (MQ=255)                                                                                                                                        ggggATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATc                                                                                                                                                                                                      <  1:925489/134‑1 (MQ=255)                                                                                                                                        ggggATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCtt                                                                                                                  >  1:625018/1‑218 (MQ=255)                                                                                                                                                  tggtgCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGgc                                                                                                                                      >  2:302209/1‑188 (MQ=255)                                                                                                                                                              tACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATgcg                                                                             >  2:460921/1‑233 (MQ=255)                                                                                                                                                                 tGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGGGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAAt                                                                                                                                >  2:299144/1‑179 (MQ=255)                                                                                                                                                                           ggATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGtgcccgcgaaggggttgatcagcggttttgcaggaaggcgcgaatgcgctccgccgcttcgatgcattcggccagcggtgcaaccagtgccatgcgcacgcgcccggcacccgggttcaccccatccacttcacgcgacaggtacgaccccggtaccacggttacatgctgggt                                                             >  2:747995‑M1/1‑62 (MQ=255)                                                                                                                                                                           ggATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGCAccc                                                             >  1:830841/1‑236 (MQ=255)                                                                                                                                                                                tgagtgagGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCGGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAATGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCaa                                                                                          >  1:319300/1‑202 (MQ=255)                                                                                                                                                                                    tggggCGAGCCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCGACAGGTACTGCGCTGCGCTTGAAAGCAgcgc                                                                                                                                                                                                 <  2:77424/92‑1 (MQ=38)                                                                                                                                                                                       ggCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGtgcccgcgaaggggttgatcagcggttttgcaggaaggcgcgaatgcgctccgccgcttcgatgcattcggccagcggtgcaaccagtgccatgcgcacgcgcccggcacccgggttcaccccatccacttcacgcgacaggtacgaccccgg                                                                                  >  1:126571‑M1/1‑50 (MQ=255)                                                                                                                                                                                         cGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGtgcccgcgaaggggttgatcagcggttttgcaggaaggcgcgaatgcgctccgccgcttcgatgcattcggccagcggtgcaaccagtgccatgcgcacgcgcccggcacccgggttcaccccatccacttcacgcgacaggtacgaccccggtaccacggttacatgctgggcttcgaacaggtcg                                                >  1:958123‑M1/1‑48 (MQ=255)                                                                                                                                                                                         cGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCAc                                                                          >  1:57324/1‑209 (MQ=255)                                                                                                                                                                                         cGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTACCACAGGTACTGCGCTGCGCTTGAGATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGGTCAGCg                                                                                                                                                     >  1:727095/1‑134 (MQ=255)                                                                                                                                                                                               tGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGtgcccgcgaaggggttgatcagcggttttgcaggaaggcgcgaatgcgctccgccgcttcgatgcattcggccagcggtgcaaccagtgccatgcgcacgcgcccggcacccgggttcaccccatccacttcacgcgacaggtacgaccccggtaccacggttacatgctgggcttcgaacaggtcgcggg                                            >  2:669594‑M1/1‑42 (MQ=255)                                                                                                                                                                                                          aaGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGCACCCGGGTTCACCCCATCCa                                             >  2:373429/1‑221 (MQ=255)                                                                                                                                                                                                          aaGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGCACCCGGGTTCACCCCATCCACTTCACGCGACAgg                               <  1:1041025/235‑1 (MQ=255)                                                                                                                                                                                                                     aTCAAGGCCCTTCGCGGGCGtgcccgcgaaggggttgatcagcggttttgcaggaaggcgcgaatgcgctccgccgcttcgatgcattcggccagcggtgcaaccagtgccatgcgcacgcgcccggcacccgggttcaccccatccacttcacgcgacaggtacgaccccggtaccacggttacatgctgggcttcgaacaggtcgcgggtgaactcggcatcgtcgcccggtac                   >  1:962437‑M1/1‑20 (MQ=255)                                                                                                                                                                                                                     aTCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGAGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTg                                                                                    >  1:15493/1‑171 (MQ=255)                                                                                                                                                                                                                      tCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGCACCCGGGTTCACCCCATCCACTTCACGCGACAGGTACGACCCCGGTa                  <  1:449497/236‑1 (MQ=255)                                                                                                                                                                                                                       cAAGGCCCTTCGCGGGCGtgcccgcgaagggggtgatcagcggttttgcaggaaggcgcgaatgcgctcctccgcttcgatgcattcggccagcggtgcaaccagtgccatgcgcacgcgcccggcacccgg                                                                                                                         >  1:478977‑M1/1‑18 (MQ=255)                                                                                                                                                                                                                       cAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGCACCCGGGTTCACCCCATCCAc                                            >  2:772843/1‑209 (MQ=255)                                                                                                                                                                                                                        aaGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGCACCCGGGTTCACCCCATCCACTTCACGCGACAGGTACGACCCCGGtg                  >  2:449494/1‑233 (MQ=255)                                                                                                                                                                                                                          ggCCCTTCGCGGGCGtgcccgcgaaggggttgatcagcggttttgcaggaaggcgcgaatgcgctccgccgcttcgatgcattcggccagcggtgcaaccagtgccatgcgcacgcgcccggcacccgggttcaccccatccacttcacgcgacaggtacgaccccggtaccacggttacatgctgggcttcgaacaggtcgcgggtgaactcggcatcgtcgcccggtaccttg               <  1:747998‑M1/235‑221 (MQ=255)                                                                                                                                                                                                                                tCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCACCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTc                                                                                                                          <  2:727092/122‑1 (MQ=255)                                                                                                                                                                                                                                gcgcggGCGtgcccgcgaaggggttgatcagcggttttgcaggaaggcgcgaatgcgctccgccgcttcgatgcattcggccagcggtgcaaccagtgccatgcgcacgcgcccggcacccgggttcaccccatccacttcacgcgacaggtacgaccccggtaccacggttacatgctgggcttcgaacaggtcgcgggtgaactcggcatcgtcgcccggtaccttggc             <  1:791993‑M1/230‑223 (MQ=255)                                                                                                                                                                                                                                  gcgGGCGtgcccgcgaaggggttgatcagcggttttgcaggaaggcgcgaatgcgctccgccgcttcgatgcattcggccagcggtgcaaccagtgccatgcgcacgcgcccggcacccgggttcaccccatccacttcacgcgacaggtacgaccccggtaccacggttacatgctgggcttcgaacaggtcgcgggtg                                          >  2:540418‑M1/1‑7 (MQ=255)                                                                                                                                                                                                                                  gcgGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGCACCCGGG‑TCACCCCATCCACTTCACGCGACAGGTAc                            <  1:358023/213‑1 (MQ=255)                                                                                                                                                                                                                                  gcgGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGCACCCGGG‑TCACCCCATCCACTTCACGCGACAGGTAc                            >  2:358020/1‑213 (MQ=255)                                                                                                                                                                                                                                  gcgGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCATCGATGCATTCGGCCAGCGGTGCAACCACTGCCATGCGCACGCGCCCGGCACCCggg                                                          >  1:663220/1‑184 (MQ=255)                                                                                                                                                                                                                                   cgGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGCACCCGGGTTCACCCCATCCACTTCACGCGACAGGTACGACCCCGGTACCACGGTTACAt      <  1:460924/235‑1 (MQ=255)                                                                                                                                                                                                                                    gggCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGCACCCggg                                                          >  2:407850/1‑182 (MQ=255)                                                                                                                                                                                                                                    gggCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGAGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAAGGCGCTCCGCCGCTTCGATGCATTCGGcc                                                                                                    >  2:497944/1‑140 (MQ=255)                                                                                                                                                                                                                                      gCGtgcccgcgaaggggttgatcagcggttttgcaggaaggcgcgaatgcgctccgccgcttcgatgcattcggccagcggtgcaaccagtgccatgcgcacgcgcccggcacccgggttcaccccatccacttcacgcgacaggtacgaccccggtaccacggttacatgctgggcttcgaacaggtcgcgggtgaactcggcatcgtcgcccggtaccttggcccacg        >  1:5844‑M1/1‑3 (MQ=255)                                                                                                                                                                                                                                      gCGtgcccgcgaaggggttgatcagcggttttgcaggaaggcgcgaatgcgctccgccgcttcgatgcattcggccagcggtgcaaccagtgccatgcgcacgcgcccggcacccgggttcaccccatccacttcacgcgacaggtacgaccccggtaccacggttacatgctgggcttcgaacaggtcgcgggtgaactcggcatcgtcgcccggtaccttggcccacaggtaga  >  1:365480‑M1/1‑3 (MQ=255)                                                                                                                                                                                                                                       cGtgcccgcgaaggggttgatcagcggttttgcaggaaggcgcgaatgcgctccgccgcttcgatgcattcggccagcggtgcaaccagtgccatgcgcacgcgcccggc                                                                                                                               <  2:673042‑M1/110‑109 (MQ=255)                                                                                                                                                                                                                                       cGtgcccgcgaaggggttgatcagcggttttgcaggaaggcgcgaatgcgctccgccgcttcgatgcattcggccagcggtgcaaccagtgccatgcgcacgcgcccggc                                                                                                                               >  1:673045‑M1/1‑2 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          CTGGAAAGCTACGACGACAGTGCTGAAGCCAAAGCCCTGGCCGCACAGATGGACTTGCTGGAGCGGCGTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGCACCCGGGTTCACCCCATCCACTTCACGCGACAGGTACGACCCCGGTACCACGGTTACATGCTG  >  AE015451/1779835‑1780299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.