| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| JC | AE015451 | 4,980,585 | +GGC | 100% | intergenic (‑24/+44) | PP_4387 ← / ← flgE | conserved exported protein of unknown function/Flagellar hook protein FlgE |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | AE015451 | = 4980585 | 0 (0.000) | 27 (0.480) +GGC |
23/336 | NT | 100% | intergenic (‑24/+44) | PP_4387/flgE | conserved exported protein of unknown function/Flagellar hook protein FlgE |
| ? | AE015451 | 4980586 = | 0 (0.000) | intergenic (‑25/+43) | PP_4387/flgE | conserved exported protein of unknown function/Flagellar hook protein FlgE | |||||
GCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCGCCGATCACCGG > AE015451/4980382‑4980814 | gCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaa > 1:818941‑M1/1‑204 (MQ=255) gttgtAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctt > 1:714310‑M1/1‑192 (MQ=255) gTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgc > 2:743530‑M1/1‑177 (MQ=255) cACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgat < 1:870160‑M1/235‑65 (MQ=255) tGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaac < 1:28085‑M1/187‑28 (MQ=255) gCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtgg < 1:743533‑M1/236‑91 (MQ=255) acacAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggt < 2:746785‑M1/193‑53 (MQ=255) gCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaa > 2:1073458‑M1/1‑132 (MQ=255) tGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgct > 2:533211‑M1/1‑124 (MQ=255) ggggcagcgggggcagGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatgaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttac > 2:586564‑M1/1‑92 (MQ=255) ggggcagcgggggcagGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttacca < 1:11618‑M1/236‑145 (MQ=255) ggggcagcgggggcagGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctgg > 1:756419‑M1/1‑92 (MQ=255) agAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtcgttgctctgcg > 1:1054528‑M1/1‑74 (MQ=255) cagcaccagcaAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttgc > 1:109245‑M1/1‑69 (MQ=255) agcaccagcaAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctcgccggtcaggtcgacgttg < 1:774189‑M1/235‑168 (MQ=255) gggAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctgctagttgctctgcgccttgatcaggtttaccagctcgccggtcaggtcgacgttggagtcctccaggg < 1:586567‑M1/236‑181 (MQ=255) ccAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctcgccggtcaggtcgacgttggagtcctccagggcaccaccgga > 1:745016‑M1/1‑46 (MQ=255) tAACGACAGCCGCTTCATTCGCGTGAACCTTCACAGTCTTGGggccgctttgcgg < 2:228747‑M1/55‑14 (MQ=255) aCGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctcgccggtcaggtcgacgttggaggcctccagggcaccaa > 1:130088‑M1/1‑40 (MQ=255) gcccgccgcTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctcgccggtcaggtcgacgttggagtcctccagggcaccaccggaaatctggc < 2:255126‑M1/232‑199 (MQ=255) gCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccgattacaaacaccaaag < 2:559783‑M1/68‑35 (MQ=255) gCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaag > 1:559786‑M1/1‑34 (MQ=255) cATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggcccccattacaaacaccaaagccaatacaatcatcaggtcatctgcatgatggtctgcatgatggtgctttcagtggacatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctcgc > 2:71086‑M1/1‑27 (MQ=255) cATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctcgccggtcaggtcgacgttggagtcctccagggcacca > 1:975373‑M1/1‑27 (MQ=255) cATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctct > 2:140436‑M1/1‑27 (MQ=255) cTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcctctgaatgatggtctgca > 2:134642‑M1/1‑14 (MQ=255) aTTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctcgccggtcaggtcgacgttg < 2:456313‑M1/175‑168 (MQ=255) aTTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctcgccggtcaggtcgacgttg > 1:456316‑M1/1‑8 (MQ=255) tCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctcgccggtcaggtcgacgttggagtcctccagggcaccaccggaaatctggccgagggtaccggtgtccggagcgccgatcac < 1:1073461‑M1/235‑230 (MQ=255) tGGggccgctttgcggccccaattacaaacaccaaagcca > 2:1022292‑M1/1‑3 (MQ=255) tGGggccgctttgcggccccaattacaaacaccaaagcca < 1:1022295‑M1/40‑38 (MQ=255) | GCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCGCCGATCACCGG > AE015451/4980382‑4980814 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |