Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	AE015451	5,201,568	Δ98 bp	39.2%	intergenic (‑54/‑29)	PP_4582 ← / → PP_4583	conserved membrane protein of unknown function/putative Peptidase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 5201567	32 (0.560)	14 (0.250)	12/336	NT	39.2%	intergenic (‑53/‑127)	PP_4582/PP_4583	conserved membrane protein of unknown function/putative Peptidase
	?	AE015451	5201666 =	12 (0.210)					intergenic (‑152/‑28)	PP_4582/PP_4583	conserved membrane protein of unknown function/putative Peptidase

CGAAGCTGTCCTTGACCGGGTCGACGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGCGATCCCTGTTGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTAC  >  AE015451/5201334‑5201792                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                  cGAAGCTGTCCTTGACCGGGTCGACGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGgcgc                                                                                                                                                                                                                                 <  1:671485/236‑1 (MQ=255)            ttGACCGGGTCGACGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGgcgc                                                                                                                                                                                                                                 >  1:893885/1‑225 (MQ=255)            ttGACCGGGTCGACGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGgcgc                                                                                                                                                                                                                                 <  2:893882/225‑1 (MQ=255)                      cgacgaTGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGcc                                                                                                                                                                                                             >  1:618836/1‑235 (MQ=255)                      cgacgaTGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGc                                                                                                                                                                                                              >  2:463140/1‑234 (MQ=255)                          gaTGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGgcgc                                                                                                                                                                                                                                 <  1:1017829/211‑1 (MQ=255)                                       agcagcagcACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTaa                                                                                                                                                                                            <  1:551044/235‑1 (MQ=255)                                       agcagcagcACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTaa                                                                                                                                                                                            <  1:1048230/235‑1 (MQ=255)                                                 ccGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTaaa                                                                                                                                                                                           <  1:785063/226‑1 (MQ=255)                                                  cGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGgcgc                                                                                                                                                                                                                                 <  1:142179/187‑1 (MQ=255)                                                   gCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGgcgc                                                                                                                                                                                                                                 <  2:908170/186‑1 (MQ=255)                                                       gCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCtg                                                                                                                                                                                                                 >  2:708753/1‑198 (MQ=255)                                                        caccacGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCACCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTaaa                                                                                                                                                                                           <  2:572433/219‑1 (MQ=255)                                                         accacGCGGAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACGGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCAAcagggat                                                                                                                                                                          <  2:13115/235‑7 (MQ=255)                                                            acGCGTAGCGGGTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCacagg                                                                                                                                                                                                                              <  1:603990‑M1/180‑6 (MQ=255)                                                             cgcgTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCctct                                                                                                                                                                                                      <  1:182524/203‑1 (MQ=255)                                                               cgTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCacaggcaactccaatcaagagaccttcaatgccaaccaag                                                                                                                                                                                           <  2:711844‑M1/212‑41 (MQ=255)                                                               cgTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCacaggcaactccaatcaagagaccttcaatgccaaccaag                                                                                                                                                                                           >  1:711847‑M1/1‑172 (MQ=255)                                                                     ggTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGCGATCCCTGtt                                                                                                                                                              <  1:546614/235‑1 (MQ=255)                                                                        tCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGc                                                                                                                                                                        <  1:933616/222‑1 (MQ=255)                                                                          gcgcAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCGGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCacaggcaactccaatcaagagaccttcaatgccaaccaagccccaacccccgatt                                                                                                                                                                            >  1:173444‑M1/1‑161 (MQ=255)                                                                           cgcAGCCAGCGCCACATCCAGCGGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACGGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGgcgc                                                                                                                                                                                                                                 <  2:4549/162‑1 (MQ=255)                                                                           cgcAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTaa                                                                                                                                                                                            <  2:368359/199‑1 (MQ=255)                                                                                     gcgacaTCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGgcgc                                                                                                                                                                                                                                 <  2:1026917/149‑1 (MQ=255)                                                                                       cacaTCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAg                                                                                                                                                                                                                               <  2:154246/152‑1 (MQ=255)                                                                                       cacaTCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAg                                                                                                                                                                                                                               >  1:154248/1‑152 (MQ=255)                                                                                             cAGCCGGTGAGCTTGAAGCCCACCGGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCacaggcaactccaatcaagagaccttcaatgccaaccaagcc                                                                                                                                                                                         <  2:173442‑M1/184‑43 (MQ=255)                                                                                             cAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCgg                                                                                                                                                                                                >  1:287292/1‑177 (MQ=255)                                                                                                 cGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGCGATCCCTGTTGGAGCgg                                                                                                                                                       >  1:390649/1‑214 (MQ=255)                                                                                                    tGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGCGATCCCTGTTgg                                                                                                                                                            <  2:889776/206‑1 (MQ=255)                                                                                                      aGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCacaggcaactccaatcaagagaccttcaatgccaaccaagccccaacccccgattgcccaagccgaccaggccaccgacccgtatgcctggctgcaacagcg                                                                                                                             <  1:817682‑M1/235‑103 (MQ=255)                                                                                                            aaGCCCGCCAGCAGGCACCCTAAGGCGGCGACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCacaggcaactccaatcaagagaccttcaatgccaaccaagccccaacccccgattgcccaagccgaccaggccaccgacccgtatgcctggctgcaacagcg                                                                                                                             <  1:158907‑M1/229‑103 (MQ=255)                                                                                                                     agcagGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCacaggcaactccaatcaagagaccttcaatgccaaccaagccccaacgac                                                                                                                                                                                 <  2:689944‑M1/168‑51 (MQ=255)                                                                                                                                           cTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAcccc                                                                                                                                                                                        >  2:99730/1‑139 (MQ=255)                                                                                                                                           cTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGCGATCCCTGTTGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGcc                                                                                       >  1:272736/1‑236 (MQ=255)                                                                                                                                           cTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCAAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTcgcg                                                                                                                                                                                                 >  2:774388/1‑130 (MQ=255)                                                                                                                                           cTGCGTACAAGCACCAGGCGAAGGTGTACTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGCCCTCTTCACGGGTAAAc                                                                                                                                                                                          >  1:761596/1‑137 (MQ=255)                                                                                                                                                 aCAAGCCCCAAGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTCTTCCGGGCacagggaactccaataaagagaccttcaatgccaaccaagccccaacccc                                                                                                                                                                                 >  2:550433‑M1/1‑90 (MQ=255)                                                                                                                                                         cAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGCGATCCCTGTTGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGCCCCAAccccc                                                                              >  1:1010459/1‑231 (MQ=255)                                                                                                                                                           ggCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCacaggcaactccaatcaagagaccttcaatgccaaccaagccccaacccccgattgcccaagccgaccaggccaccgacccgtatgcctggctgcaacagcgcgacacccccgaggtactcgcttacctgcaagct                                                                                           <  1:986128‑M1/216‑137 (MQ=255)                                                                                                                                                            gCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGCGATCCCTGTTGGAGCGGGTTTAccc                                                                                                                                               <  1:813078/163‑1 (MQ=255)                                                                                                                                                             cGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGCg                                                                                                                                                                       >  2:624388/1‑138 (MQ=255)                                                                                                                                                                 ggTGTAGTCGGTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCacaggcaactccaatcaagagaccttcaatgccaaccaagccccaacccccgattgcccaagccgaccaggcca                                                                                                                                                         <  1:517189‑M1/148‑75 (MQ=255)                                                                                                                                                                   tgtAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCacaggcaactccaatcaagagaccttcaatgccaaccaagccccaacccccgattgcccaagccgaccaggccaccgacccgtatgcctggctgcaacagcgcgacacccccgaggtactcgcttacctgcaagctga                                                                                         <  1:1089875‑M1/210‑139 (MQ=255)                                                                                                                                                                   tgtAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGCGATCCCTGTTGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCGa                                                               >  1:151933/1‑236 (MQ=255)                                                                                                                                                                         cGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCTCCAGATAAGCCGCTCCTGcc                                                                                                                                                                               >  2:813075/1‑118 (MQ=255)                                                                                                                                                                         cGTCCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGACATGCCTACGATCATCACGTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTGGCGGGTAAACCCGCTCCTCCCTCGGTGCGATCCCTGTTGGAGCGGGTTTACCcgc                                                                                                                                             <  2:482260/152‑1 (MQ=255)                                                                                                                                                                                ggTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGCGATCCCTGTTGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCCGGCaa                                                                                                                         >  2:1089872/1‑165 (MQ=255)                                                                                                                                                                                 gTCATGGTGGTCGTGCAAGCCAGGCAAAGAGAGGCCTATGATAAGCGCTTTGCCGGGCACAGGCaact                                                                                                                                                                                                                         <  2:247378/68‑4 (MQ=255)                                                                                                                                                                                  tCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTGGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGCGATCCCTGTTGGAGCGGGTTTACCcgc                                                                                                                                             <  2:107224/143‑1 (MQ=255)                                                                                                                                                                                    aTGGTGTTCGGGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCtc                                                                                                                                                                                                                 >  2:176645/1‑72 (MQ=255)                                                                                                                                                                                               gCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCaca                                                                                                                                                                                                                                <  2:720857‑M1/47‑4 (MQ=255)                                                                                                                                                                                               gCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCa                                                                                                                                                                                                                                >  1:720860/1‑47 (MQ=255)                                                                                                                                                                                               gCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGCGATCCCTGTTGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTg                                   >  1:684469/1‑236 (MQ=255)                                                                                                                                                                                                       ggCAAAGAGATGCCTATGATAAAAACTTTTCCGGGCacaggcaactccaatcaagagaccttcaatgccaatcaagccccaacccccgattgcccaagccgaccaggccaccgacccgtatgcc                                                                                                                                           >  2:411580‑M1/1‑36 (MQ=255)                                                                                                                                                                                                            agagagGCCTATGATACACACTTTTCCGGGCacaggcagctccaatcaagagaccttcaatgccaaccaagccccaacccccgattgcccaagccgaccaggccaccgacccgtatgcctggc                                                                                                                                       <  2:87973‑M1/123‑93 (MQ=255)                                                                                                                                                                                                                        gATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCCCCTACCTCGGTGCGATCCCTGTTGGAg                                                                                                                                                          >  1:762557/1‑92 (MQ=255)                                                                                                                                                                                                                                cTTTTCCGGGCacaggcaactccaatcaagagaccttcaatgccaaccaagccccaacccccgattgcccaagccgaccaggccaccgacccgtatgcctggctgcaacagcgcgacacccccgaggtactcgcttacctgcaagctgaaaacgcctaccaggaagcctgcctggccgacca                                                        >  1:68660‑M1/1‑11 (MQ=255)                                                                                                                                                                                                                                cTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACTTCGGTGCGATCCCTGTTGGAGCggg                                                                                                                                                      >  1:898054/1‑88 (MQ=255)                                                                                                                                                                                                                                    tgcggGCacaggcaactccaatcaagagaccttcaatgccaaccaagccccaacccccgattgcccaagccgaccaggccaccgacccgta                                                                                                                                               <  1:1018666‑M1/89‑85 (MQ=255)                                                                                                                                                                                                                                    ggcggGCacaggcaactccaatcaagagaccttcaatgccaaccaagccccaacccccgattgcccaagccgaccaggccaccgacccgtatgcctggctgcaacagcgcgacacccccgaggtactcgcttacctgcaagctgaaaacgcctaccaggaagcctgcctggccgaccaggcaccgttgcgtgagcgcctgttcgaagaaatcaagggccgcatcctggagac  <  1:655805‑M1/230‑226 (MQ=255)                                                                                                                                                                                                                                     ccGGGCacaggcaactccaatcaagagaccttcaatgccaaccaagccccaacccccgattgcccaagccgaccaggccaccgacccgtatgcctggctgcaacagcgcgacacccccgaggtactcgcttacctgcaagctgaaaacgcctaccaggaagcctgcctggccgaccaggcaccgttgcgcgagcg                                      >  2:850568‑M1/1‑6 (MQ=255)                                                                                                                                                                                                                                      cGGGCacaggcaactccaatcaagagacc                                                                                                                                                                                                           <  2:390646‑M1/29‑25 (MQ=255)                                                                                                                                                                                                                                      cGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGCGATCCCTGTTGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGCCCCAAc                                                                                  >  2:764545/1‑150 (MQ=255)                                                                                                                                                                                                                                       gggCacaggcaactccaatcaagagaccttcaatgccaaccaagccccaacccccgattgcccaagccgaccaggccaccgacccgtatgcctggctgcaacagcgcgacacccccgaggtactcgcttacctgcaagctgaaaacgcctaccaggaagcctgcctggccgaccaggcaccgttgcgtgagcgcctgttcgaagaaatcaagggccg              <  1:463143‑M1/217‑214 (MQ=255)                                                                                                                                                                                                                                       gggCacaggcaactccaatcaagagaccttcaatgccaaccaagccccaacccccgattgcccaagccgaccaggccaccgacccgtatgcctggctgcaa                                                                                                                                  <  1:641430‑M1/101‑98 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                  CGAAGCTGTCCTTGACCGGGTCGACGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGCGATCCCTGTTGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTAC  >  AE015451/5201334‑5201792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.