Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	AE015451	4,808,175	Δ14 bp	100%	intergenic (‑284/‑258)	PP_4234 ← / → dsbD‑II	Aldehyde oxidase and xanthine dehydrogenase/thiol/disulfide interchange protein 2

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 4808174	0 (0.000)	5 (0.090)	5/326	NT	100%	intergenic (‑283/‑272)	PP_4234/dsbD‑II	Aldehyde oxidase and xanthine dehydrogenase/thiol/disulfide interchange protein 2
	?	AE015451	4808189 =	0 (0.000)					intergenic (‑298/‑257)	PP_4234/dsbD‑II	Aldehyde oxidase and xanthine dehydrogenase/thiol/disulfide interchange protein 2

GCTGACAATGTGTCGCGGTGTGTATAGGGCAATGGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCCAACAACAAAGACCCTGCAAACCCAGAACAAACTCTTACTACTCCTG  >  AE015451/4808007‑4808361                                                                                                                                                                                       |                                                                                                                                                                             agaggcaaagtcaagctgacaatgtgtcgcggtgtgtatagggcaatgggcggaattcgcgctttggcgggtaggtgtttggcgggaggttttcagcgcctgggaggtgttcgccgggggattttcagcgcctgggaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcGCGGGCGGCGCTc                                                                                                                                                                  >  1:860415‑M2/183‑195 (MQ=255)                                                  gcggaattcgcgctttggcgggtaggtgtttggcgggaggttttcagcgcctgggaggtgttcgccgggggattttcagcgcctgggaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCt                                                                                                                             <  2:699697‑M2/50‑1 (MQ=255)                                                  gcggaattcgcgctttggcgggtaggtgtttggcgggaggttttcagcgcctgggaggtgttcgccgggggattttcagcgcctgggaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCt                                                                                                                             >  1:699700‑M2/134‑183 (MQ=255)                                                       gatttgcgcgcgaccgccttggggcatgtctcgatatcgcgtcgtacaaacaaggcggtcgcgcgcgactggtgcaggagggactggccgaaacaaacgtaggagcgggtttACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCCAAAttcaa                                                                                             <  1:62589‑M1/98‑3 (MQ=255)                                                       gatttgcgcgcgaccgccttggggcatgtctcgatatcgcgtcgtacaaacaaggcggtcgcgcgcgactggtgcaggagggactggccgaaacaaacgtaggagcgggtttACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTccacc                                                                     <  1:19803‑M1/122‑3 (MQ=255)                                                                                    ctcgatatcgcgtcgtacaaacaaggcggtcgcgcgcgactggtgcaggagggactggccgaaacaaacgtaggagcgggtttACCCGCGGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCCCTCaaaa                                                                                                  <  2:125031‑M1/93‑1 (MQ=255)                                                                                              cgtcgtacaaacaaggcggtcgcgcgcgactggtgcaggagggactggccgaaacaaacgtaggagcgggtttACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCCAACAACAAAGAccc                                  >  1:125033‑M1/74‑230 (MQ=255)                                                                                                                         gactggtgcaggagggactggccgaaacaaacgtaggagcgggtttACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCCAACAACAAAGACCCTGCAAACCCAGAACAAACTCTTACTACTCCTg  >  1:415166‑M1/47‑235 (MQ=255)                                                                                                                                     agggactggccgaaacacacgtaggagcgggtttACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCCAACAACAAAGACCCTGCAAACCCAGaa                     >  1:962472‑M1/35‑204 (MQ=255)                                                                                                                                      gggactggccgaaacaaacgtaggagcgggtttACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAAt                                                  <  1:679281‑M1/141‑1 (MQ=255)                                                                                                                                      gggactggccgaaacaaacgtaggagcgggtttACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAAt                                                  >  2:679278‑M1/34‑174 (MQ=255)                                                                                                                                      gggactggccgaaacaaacgtaggagcgggtttACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCCAACAACAAAGACCCTGCAAACCCAGAACAAACTCTtact         <  2:415163‑M1/182‑1 (MQ=255)                                                                                                                                            ggccgaaacaaacgtaggagcgggtttACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAACCACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCaa                                                                              <  2:51421‑M1/113‑1 (MQ=255)                                                                                                                                            ggccgaaacaaacgtaggagcgggtttACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCACAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCCAACAACAAAGACCCTGCAAACCCAGAACAAACTCTTACTACTc     <  2:401835‑M1/186‑1 (MQ=255)                                                                                                                                            ggccgaaacaaacgtaggagcgggtttACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCCAACAACAAAGACCCTGCAAACCCAGAACAAActc              >  1:377349‑M1/28‑204 (MQ=255)                                                                                                                                            ggccgaaacaaacgtaggagcgggtttACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCCAACAACAAAGACCCTGCAAACCCAGAACAAActc              <  2:377346‑M1/177‑1 (MQ=255)                                                                                                                                                 aaacaaacgtaggagcgggtttACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATcc                                                <  1:570717‑M1/143‑1 (MQ=255)                                                                                                                                                 aaacaaacgtaggagcgggtttACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATcc                                                >  2:570714‑M1/23‑165 (MQ=255)                                                                                                                                                        gagtttcagcgcctgggagatcgagcgccgcCCGCGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCCAACAACAAAGACCCTGCAAACCCAGAACAAActct             <  1:498944‑M2/162‑1 (MQ=255)                                                                                                                                                                      gggagatcgagcgccgcGCGGGCGGCGCTCGATCTCACAGGCGCGGGAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCcaacaaca                                         >  2:336935‑M2/18‑151 (MQ=255)                                                                                                                                                                                     tcGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCCAACAACAAAGACCCTGCaaa                            <  2:377534‑M2/147‑1 (MQ=255)                                                                                                                                                                                       |                                                                                                                                                                             GCTGACAATGTGTCGCGGTGTGTATAGGGCAATGGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCACTCAAAATTCCAACGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCCAACAACAAAGACCCTGCAAACCCAGAACAAACTCTTACTACTCCTG  >  AE015451/4808007‑4808361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.