| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 6,050,487 | C→G | 6.0% | P450R (CCG→CGG) | spoT → | bifunctional (p)ppGpp synthase/hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 6,050,487 | 0 | C | G | 6.0% | 234.9 / 7.4 | 67 | P450R (CCG→CGG) | spoT | bifunctional (p)ppGpp synthase/hydrolase |
| Reads supporting (aligned to +/- strand): ref base C (25/38); new base G (2/2); total (27/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.27e-02 | |||||||||||
AGCGTGAAGATCGACCTGTTCCCGGACGAGGTCTACGTGTTCACGCCCAAAGGCCGGATCATGGAGTTGCCCAAAGGCTCCACGGCCGTCGACTTCGCCTACGCGGTCCACACCGACGTCGGCAACAGTTGCATCGCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCTCGGAGTCCATCAGCCTGGGCGAGCGCCTGCTGAACAAGGTACTCACTGGCTTCGACAGCAGCCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAG > AE015451/6050273‑6050720 | agCGTGAAGATCGACCTGTTCCCGGACGAGGTCTACGTGTTCACGCCCAAAGGCCGGATCATGGAGTTGCCCAAAGGCTCCACGGCCGTCGACTTCGCCTACGCGGTCCACACCGACGTCGGCAACAGTTGCATCGCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAAcc < 1:214639/232‑1 (MQ=255) gTGAAGATCGACCTGTTCCCGGACGAGGTCTACGTGTTCACGCCCAAAGGCCGGATCATGGAGTTGCCCAAAGGCTCCACGGCCGTCGACTTCGCCTACGCGGTCCACACCGACGTCGGCAACAGTTGCATCGCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATg > 1:1042024/1‑236 (MQ=255) aCCTGTTCCCGGACTAGGTCTACGTGTTCACGCCCAAAGGCCGGATCATGGAGTTGCCCAAAGGCTCCACGGCCGTCGACTTCGCCTACGCGGTCCACACCGACGTCGGCAACAGTTGCATCGCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACt < 1:559440/234‑1 (MQ=255) ccGGACGAGGTCTACGTGTTCACGCCCAAAGGCCGGATCATGGAGTTGCCCAAAGGCTCCACGGCCGTCGACTTCGCCTACGCGGTCCACACCGACGTCGGCAACAGTTGCATCGCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGtct > 2:865147/1‑235 (MQ=255) tACGTGTTCACGCCCAAAGGCCGGATCATGGAGTTGCCCAAAGGCTCCACGGCCGTCGACTTCGCCTACGCGGTCCACACCGACGTCGGCAACAGTTGCATCGCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCACACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGgcacg > 1:89142/1‑236 (MQ=255) gCCCAAAGGCCGGATCATGGAGTTGCCCAAAGGCTCCACGGCCGTCGACTTCGCCTACGCGGTCCACACCGACGTCGGCAACAGTTGCATCGCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAgg > 1:20381/1‑221 (MQ=255) gCCCAAAGGCCGGATCATGGAGTTGCCCAAAGGCTCCACGGCCGTCGACTTCGCCTACGCGGTCCACACCGACGTCGGCAACAGTTGCATCGCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATc > 1:717280/1‑235 (MQ=255) aTCATGGAGTTGCCCAAAGGCTCCACGGCCGTCGACTTCGCCTACGCGGTCCACACCGACGTCGGCAACAGTTGCATCGCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCa > 1:915295/1‑235 (MQ=255) aTGGAGTTGCCCAAAGTCTCCACGGCCGTCGACTTCGCCTACGCGGTCCACACCGACGTCGGCAACAGTTGCATCGCTTGCCGCATCACCCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCg < 2:348387/174‑1 (MQ=255) tGGAGTTGCCCAAAGGCTCCACGGCCGTCGACTTCGCCTACGCGGTCCACACCGACGTCGGCAACAGTTGCATCGCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTgga > 2:126498/1‑192 (MQ=255) ggAGTTGCCCAAAGGCTCCACGGCCGTCGACTTCGCCTACGCGGTCCACACCGACGTCGGCAACAGTTGCATCGCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAg < 1:491650/202‑1 (MQ=255) cAAAGGCTCCACGGCCGTCGACTTCGCCTACGCGGTCCACACCGACGTCGGCAACAGTTGCATCGCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGCAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGgcacgc > 1:351011/1‑199 (MQ=255) aaaGGCTCCACGGCCGTCGACTTCGCCTACGCGGTCCACACCGACGTCGGCAACAGTTGCATCGCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCTc > 1:22549/1‑236 (MQ=255) aaGGCTCCACGGCCGTCGACTTCGCCTACGCGGTCCACACCGACGTCGGCAACAGTTGCATCGCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCTc < 1:625878/235‑1 (MQ=255) aGGCTCCACGGCCGTCGACTTCGCCTACGCGGTCCACACCGACGTCGGCAACAGTTGCATCGCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCt < 1:90613/233‑1 (MQ=255) gcGGCCGTCGCCTTCGCCTACGCGGTCCACACCGACGTCGGCAACAGTTGCATCGCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACa < 1:315814/217‑1 (MQ=255) cGGCCGTCGACTTCGCCTACGCGGTCCACACCGACGTCGGCAACAGTTGCATCGCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAgcgc > 1:585450/1‑221 (MQ=255) cGGCCGTCGACTTCGCCTACGCGGTCCACACCGACGTCGGCAACAGTTGCATCGCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCTCGGAGTCCAt > 1:1064771/1‑235 (MQ=255) ttCGCCTACGCGGTCCACACCGACGTCGGCAACAGTTGCATCGCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCTCGGAGTCCATCAGCCTGGGCg < 1:523121/235‑1 (MQ=255) tCGCCTACGCGGTCCACACCGACGTCGGCAACAGTTGCATCGCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCGCGGGCGCTCGGCCAAACCCGGCATg < 2:665770/145‑1 (MQ=255) gCCCACGCGGTCCACACCGACGTCGGCAACAGTTGCATCGCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCTCGGAGTCCATCAGCCTGGGCg < 1:189844/232‑1 (MQ=255) ccTACGCGGTCCACACCGACGTCGGCAACAGTTGCATCGCTTGCCGCATCACCCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTtgtg < 2:749087/155‑1 (MQ=255) ccTACGCGGTCCACACCGACGTCGGCAACAGTTGCATCGCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCTCGGAGTCCATCAGCCTGGGCGAgcgc < 1:244856/236‑1 (MQ=255) cTACGCGGTCCACACCGACGTCGGCAACAGTTGCATCGCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCaa > 1:1077035/1‑165 (MQ=255) caCGCGGTCCACACCGACGTCGGCAACAGTTGCATCGCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCTCGGAGTCCATCAGCCTGGGCg < 1:102550/228‑1 (MQ=255) ggTCCACACCGACGTCGGCAACAGTTGCATCGCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGGGAAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCTc < 2:1034330/204‑1 (MQ=255) gTCCACACCGACGTCGGCAACAGTTGCATCGCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCTCGGAGTCCATCAGCCTGGGCGAGCGCctgctg < 2:931355/234‑1 (MQ=255) tCCACACCGACGTCGGCAACAGTTGCATCGCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCTCGGAg < 2:505071/206‑1 (MQ=255) cacaCCGACGTCGGCAACAGTTGCATCGCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCTCGGAGTCCATCAGCCTGGGCGAg < 2:429725/222‑1 (MQ=255) cGGCAACAGTTGCATCGCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCTCGGAGTCCATCAGCCTGGGCGAGCGCCTGCTGAACAAGGTACTCACTg > 1:1060817/1‑236 (MQ=255) cGGCAACAGTTGCATCGCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCCCAACTTTgggg > 2:417723/1‑134 (MQ=255) cGGCAACAGTTGCATCGCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCCCGACAGTGGAAATCGTCAGCGCCCCg > 2:338453/1‑97 (MQ=255) aCAGTTGCATCGCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCTCGGAGTCCATCAGCCTGGGCg < 2:667684/204‑1 (MQ=255) ttGCATCGCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGcacgcacg > 2:418454/1‑145 (MQ=255) cATCGCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGaa > 2:648538/1‑144 (MQ=255) aTCGCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCTCGGAGTCCATCAGCCTGGGCg < 1:78410/196‑1 (MQ=255) gCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCTCGGAGTCCATCAGCCTGGGCGAGCGCCTGCTGAACa < 1:706166/208‑1 (MQ=255) gCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCTCGGAGTCCATCAGCCTGGGCGAGCGCCTGCTGAACAAGGTACTCACTGGCTTCGa < 2:903620/223‑1 (MQ=255) aaCCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCTCGGAGTCCATCAGCCTGGGCGAGCGCCTGCTGAACAAGGTACTCACTGGCTTCGACAGCAGCCTGGAGAAAATcc < 1:865150/235‑1 (MQ=255) aCCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCTCGGAGTCCATCAGCCTGGGCg < 2:820913/180‑1 (MQ=255) ccgccgCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCTCGGAGTCCATCAGCCTGGGCGAGCGCCTGCTGAACAAGGTACTCACTGGCTTCGa < 2:982474/213‑1 (MQ=255) cgccgcCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCTCGGAGTCCATCAGCCTGGGCGAGCGCCTGCTGAACAAGGTACTCACTGGCTTCGACAGCAGCCTGGAGAAAATCCCCCa < 1:746316/236‑1 (MQ=255) cTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACCCGCTCAAGc > 2:797421/1‑139 (MQ=255) tCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCTCGGAGTCCATCAGCCTGGGCGAGCGCCTGCTGAACAAGGTACTCACTGGCTTCGACAGCAGCCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTa < 1:1021960/235‑1 (MQ=255) gCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCTCGGAGTCCATCAGCCTGGGCGAGCGCCTGCTGAACAAGGTACTCACTGGCTTCGACAGCAGCCTGGAGAAAATCCCCCAGGAACGc < 2:1064768/217‑1 (MQ=255) cTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCTCGGAGTCCATCAGCCTGGGCGAGCGCCTGCTGAACAAGGTACTCACTGGCTTCGACAGCAGCCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCgc > 1:264448/1‑235 (MQ=255) aaaGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACtgtgtg < 1:260596/71‑1 (MQ=255) aaaGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACt > 2:1063267/1‑66 (MQ=255) aaaGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACt < 1:1063270/66‑1 (MQ=255) aaaGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTtgtg > 2:260593/1‑71 (MQ=255) aaaGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAg > 2:397081/1‑119 (MQ=255) aaGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCTCGGAGTCCATCAGCCTGGGCGAGCGCCTGCTGAACAAGGTACTCACTGGCTTCGACAGCAGCCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTAcc < 1:1072454/236‑1 (MQ=255) aGCGGCTGGACAGGGGAAATCGTCAGCGCCGCGGGCGCGCGGCCAAACCCGGCATGGCTCAACTGTGTGGTCTCGGGCGAGGCACGCACGGATATCCGCCa < 2:18300/101‑1 (MQ=255) aGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCTCGGAGTCCATCAGCCTGGGCGAGCGCCTGCTGAACAAGGTACTCACTGGCTTCGAcagc < 2:717277/183‑1 (MQ=255) cTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCTCGGAGTCCATc > 1:865715/1‑130 (MQ=255) tCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCTCGGAGTCCATCAGCCTGGGCGAGCGCCTGCTGAACAAGGTACTCACTGGCTTCGACAGCAGCCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGa < 1:567621/236‑1 (MQ=255) gACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCTCGGAGTCCATCAGCCTGGGCGAGCGCCTGCTGAACAAGGTACTCACTGGCTTCGACAGCAGCCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAg > 1:753040/1‑235 (MQ=255) aCAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCTc < 2:790559/116‑1 (MQ=255) tCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCTCGGAGTCCATCAGCCTGGGCGAGCGCCTGCTGAACAAGGTACTCACTGGCTTCGACAGCAGCCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGa < 1:8197/223‑1 (MQ=255) gTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCTCGGAGTCCATCAGCCTGGGCGAGCGCCTGCTGAACAAGGTACTCACTGGCTTCGACAGCAGCCTGGAGaaaa < 2:521271/175‑1 (MQ=255) cgaGCGCCCGGGGCGCTCGGCCAAACCCGGCATGGCTCAACt < 2:943462/40‑1 (MQ=255) cgaGCGCCCGGGGCGCTCGGCCAAACCCGGCATGGCTCAACt > 1:943465/3‑42 (MQ=255) cgaGCGCCCGGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCAcgc > 2:686726/3‑82 (MQ=255) cgaGCGCCCGGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCAcgc < 1:686729/80‑1 (MQ=255) aGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCTCGGAGTCCATCAGCCTGGGCGAGCGCCTGCTGAACAAGGTACTCACTGGCTTCGACAGCAGCCTGGAGAAAATCCCCCAGGAACg < 2:1060814/185‑1 (MQ=255) cgcCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGGGAGgca > 2:490452/1‑58 (MQ=255) cgcCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCTCGGAGTCCATCAGCCTGGGCGAGCGCCTGCTGAACAAGGTACTCACTGGCTTCGACAGCAGCCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCt < 1:345949/213‑1 (MQ=255) cccGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCTCGGAGTCCATCAGCCTGGGCGAGCGCCTGCTGAACAAGGTACTCACTGGCTTCGACAGCAGCCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAg < 1:14019/236‑1 (MQ=255) | AGCGTGAAGATCGACCTGTTCCCGGACGAGGTCTACGTGTTCACGCCCAAAGGCCGGATCATGGAGTTGCCCAAAGGCTCCACGGCCGTCGACTTCGCCTACGCGGTCCACACCGACGTCGGCAACAGTTGCATCGCTTGCCGCATCAACCGCCGCCTGGCGCCGCTGTCCGAACCGCTACAAAGCGGCTCGACAGTGGAAATCGTCAGCGCCCCGGGCGCTCGGCCAAACCCGGCATGGCTCAACTTTGTGGTCTCGGGCAAGGCACGCACGAATATCCGCCACGCGCTCAAGCAACAGCGCCGCTCGGAGTCCATCAGCCTGGGCGAGCGCCTGCTGAACAAGGTACTCACTGGCTTCGACAGCAGCCTGGAGAAAATCCCCCAGGAACGCATCCAGTCTATTCTCGCCGAGTACCGCCTGGAGCTCATAGAAGACCTGCTCGAAG > AE015451/6050273‑6050720 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |