Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AE015451 6,132,525 T→C 20.9% E443G (GAG→GGG)  copA‑II ← copper resistance protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE0154516,132,5250TC20.9% 105.8 / 2.8 50E443G (GAG→GGG) copA‑IIcopper resistance protein A
Reads supporting (aligned to +/- strand):  ref base T (23/15);  new base C (8/3);  total (32/18)
Fisher's exact test for biased strand distribution p-value = 7.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGTTGGACTCATAGTCTGCATGTCGACCAAGGGGTTGTTAGTCTCTGAGGCTGGGTGAGACTGCATGGCGCCACCCATACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATACCGG  >  AE015451/6132301‑6132696
                                                                                                                                                                                                                                |                                                                                                                                                                           
gCGTTGGACTCATAGTCTGCATGTCGACCAAGGGGTTGTTAGTCTCTGAGGCTGGGTGAGACTGCATGGCGCCACCCATACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCAtttt                                                                                                                                                                   <  1:304221/235‑1 (MQ=255)
 cGTTGGACTCATAGTCTGCATGTCGACCAAGGGGTTGTTAGTCTCTGAGGCTGGGTGAGACTGCATGGCGCCACCCATACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTg                                                                                                                                                                  >  1:433100/1‑235 (MQ=255)
              gTCTGGATGTCGTCCAGGGGGTTGTTAGTCTCTGAGGCTGGGTGAGACTGCATGGCGCCACCCATACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCAtttt                                                                                                                                                                   <  1:809744/221‑1 (MQ=255)
                   cATGTCGACCAAGGGGTTGTTAGTCACTGAGGCTGGGTGAGACTGCATGGCGCCACCCATACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCg                                                                                                                                                   >  1:565519/1‑232 (MQ=255)
                        cGACCAAGGGGTTGTTAGTCTCTGAGGCTGGGTGAGACTGCATGGCGCCACCCATACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCAtct                                                                                                                                                                             >  2:608586/1‑201 (MQ=255)
                         gACCAAGGGGTTGTTAGTCTCTGAGGCTGGGTGAGACTGCATGGCGCCACCCATACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCTGCCGTGGTCCATCTCAGCCATTTTGCTGTGGTCCATGCCGGCCAtgctgc                                                                                                                                         >  1:599396/1‑236 (MQ=255)
                         gACCAAGGGGTTGTTAGTCTCTGAGGCTGGGTGAGACTGCATGGCGCCACCCATACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCAtgctg                                                                                                                                          <  1:608589/235‑1 (MQ=255)
                                   ttgttAGTCGCTGAGGCTGGGTGAGACTGCATGGCGCCACCCATACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCa                                                                                                                                <  1:589348/235‑1 (MQ=255)
                                        aGTCTCTGAGGCTGGGTGAGACTGCATGGCGCCACCCATACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATg                                                                                                                                                      >  1:1083743/1‑208 (MQ=255)
                                        aGTCTCTGAGGCTGGGTGAGACTGCATGGCGCCACCCATACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCAt                                                                                                                                              >  1:252924/1‑216 (MQ=255)
                                                aGGCTGGGTGAGACTGCATGGCGCCACCCATACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTg                                                                                                                  <  1:242305/236‑1 (MQ=255)
                                                          cgaCTGCATGGCGCCACCCATCCCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCAtttt                                                                                                                                                                   <  2:1083740/176‑1 (MQ=255)
                                                                 aTGGCGCCACCCATACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCAtgctg                                                                                                                                          <  2:760127/195‑1 (MQ=255)
                                                                   ggCGCCACCCATACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCAACCCAg                                                                                                                                                                          >  2:445357/1‑161 (MQ=255)
                                                                                 cTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCAtttt                                                                                                                   <  1:64514/202‑1 (MQ=255)
                                                                                  ttCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATACCGGCCATGCTGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATTCCGGCCa                                                                                                                                               >  2:518052/1‑173 (MQ=14)
                                                                                       tgctgcTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCTGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCTGCCGTGGTCCATCACAGCCAtttt                                                                                                                                                                   >  2:843511/1‑148 (MQ=255)
                                                                                       tgctgcTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATCCCGCCGTGGTCCCTCCCGGCCCTtgtg                                                                                                                                                                  >  2:163052/1‑149 (MQ=17)
                                                                                          tgctgTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCCTGGTCCATCCCAGCCATTTTGCCGTGGTCCa                                                                                                        >  2:1008829/1‑204 (MQ=255)
                                                                                           gctgTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCACCCATTTCGCTGTGGTCCacacc                                                                                                                                                    >  2:876750/1‑155 (MQ=255)
                                                                                                    cATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGGCCATGCCGGCCATGCGGCCGCGGTCCATCTCAGACATTTTGCTGTGATc                                                                                                                                                          >  2:208915/1‑144 (MQ=255)
                                                                                                         cGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCGTGGTCCATCCCAGCCAttt                                                                                                                    >  1:1082107/1‑177 (MQ=255)
                                                                                                               tGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCTTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCg                                                                                                   >  1:877517/1‑188 (MQ=255)
                                                                                                                gccgcaTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATcc                                                                             <  1:425410/209‑1 (MQ=255)
                                                                                                                            cATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGCGGTCCATCTCAGCCATCTTg                                                                                                                                                                  >  2:151550/1‑112 (MQ=255)
                                                                                                                             aTCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCGTGCCGCCGTGGTCCATCCCCGCCAt                                                                                                                                                                      >  2:999401/1‑107 (MQ=255)
                                                                                                                                       tCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCCCAGCCATTTTGCTGTTATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGt                                                                                                 >  2:45458/1‑166 (MQ=255)
                                                                                                                                                      tatCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGcc                                                                                                                                                                        <  2:232429/80‑1 (MQ=255)
                                                                                                                                                       atCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCCCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATCGTCCACCCCAGCCa                                                                                                                       >  2:8999/1‑128 (MQ=255)
                                                                                                                                                         cGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGc                                                     >  1:332514/1‑192 (MQ=255)
                                                                                                                                                              aTGCCGCCTTGTTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATACCAGCCATTTTTCTGTGATCCATTCCGGCCGTGCTGCCATGGTCCATCCCAGCCATTTTGCGGTTGTCCATGcc                                                                                                    >  2:735868/1‑140 (MQ=17)
                                                                                                                                                              aTGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCTGCCGTGGTCCATCTCAGTCATTTTGCtgtgg                                                                                                                                                            >  2:252983/1‑83 (MQ=255)
                                                                                                                                                                cccgccTTGGCCCATCCCCGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCAtttt                                                                                                                   <  2:370082/122‑1 (MQ=255)
                                                                                                                                                                  cgccTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCAtttt                                                                   <  1:911615/169‑1 (MQ=255)
                                                                                                                                                                   gccTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCGTGGTGCATCCCGGCCATTTTGCTGTGGTCCATGCCCGTCa                                                                                               >  1:1059847/1‑140 (MQ=255)
                                                                                                                                                                    ccTGGGTCTATCCTAGACATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCCACCATTTTGCTGTGATCCATGCCGGCCATGCCGCCATGCTCCATCCCAGcc                                                                                                                        <  1:1029815/114‑1 (MQ=255)
                                                                                                                                                                        ggTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCAtttttc                 >  1:91837/1‑211 (MQ=255)
                                                                                                                                                                                 cAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCgg                                                  >  1:610604/1‑171 (MQ=255)
                                                                                                                                                                                      aTCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCACACCgg  >  1:270027/1‑214 (MQ=255)
                                                                                                                                                                                      aTCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCAATGCCGGTCATGTTGCCGTGGTGCATCCCCGCCATTTTGCTGTGGTCCATGCCGGcc                                                >  1:430353/1‑168 (MQ=255)
                                                                                                                                                                                      aTCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATGTTGCTGTGGTCCATGCCGACCATGCTGCCATGGTCCATCCCGGCCATCTTGCTGTGGCCCATGc                                                                                                     >  2:745464/1‑115 (MQ=18)
                                                                                                                                                                                           gCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTCTGCTGTGGTCGATACCGGCCAt                                                                                              >  2:93727/1‑117 (MQ=18)
                                                                                                                                                                                           gCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCCCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATccc                                                                                                                            >  2:1030118/1‑87 (MQ=255)
                                                                                                                                                                                                  tCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCAtttt                   >  1:766429/1‑185 (MQ=255)
                                                                                                                                                                                                  tCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCATTTTGCtgt              <  1:812738/190‑1 (MQ=255)
                                                                                                                                                                                                    cATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGCTGCCGTGATCCATacccata                        >  2:456230/1‑174 (MQ=255)
                                                                                                                                                                                                      tGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGGTCCATGCCGGCCATGCTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCAtttt                   <  1:142149/181‑1 (MQ=255)
                                                                                                                                                                                                               tGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCTCTTTGccg               >  1:597545/1‑174 (MQ=255)
                                                                                                                                                                                                                  cgccgTGGTCCATGCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGt                                            <  2:599393/144‑1 (MQ=255)
                                                                                                                                                                                                                          tCCATCCCATCCTTTTTGCTGTGGGCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGt                                      <  2:496337/142‑1 (MQ=11)
                                                                                                                                                                                                                          cccATCCCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCTTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTg                                          <  1:466803/137‑1 (MQ=255)
                                                                                                                                                                                                                                |                                                                                                                                                                           
GCGTTGGACTCATAGTCTGCATGTCGACCAAGGGGTTGTTAGTCTCTGAGGCTGGGTGAGACTGCATGGCGCCACCCATACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATACCGG  >  AE015451/6132301‑6132696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: