Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AE015451 197,866 G→C 21.1% A1124A (GCG→GCC)  PP_0168 → putative surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451197,8660GC21.1% 120.6 / 20.6 47A1124A (GCG→GCC) PP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base G (23/14);  new base C (6/4);  total (29/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGACCCCAGCCTCGACTGTCATCAATGACAGCATCGATACCGTCACCGTAAGTATTGTCAGCAATGGCAATGTGACCGAAGACCAGCAGCCTTCCTTTACTGTGAAAGTAA  >  AE015451/197656‑198101
                                                                                                                                                                                                                  |                                                                                                                                                                                                                                           
cacaGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGa                                                                                                                                                                                                                      >  1:852617/1‑234 (MQ=255)
             ttCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGc                                                                                                                                                                                                       >  1:704242/1‑236 (MQ=255)
                                       gCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACg                                                                                                                                                                                               >  1:453835/1‑218 (MQ=255)
                                                                    tCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACg                                                                                                                                                >  1:505956/1‑236 (MQ=255)
                                                                     cGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACAGTCAGCGTCACCATCGAGAACGCCAccg                                                                                                                                               >  1:287648/1‑234 (MQ=11)
                                                                           cacTACCGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGc                                                                                                                                                                                                                           <  2:262919/154‑1 (MQ=255)
                                                                                     aCCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTCGAAGCGGGTAAAACCCAGGGCAGGGTCGATGTCCAGACCCCGGcc                                                                                                                                                                                                      >  1:147253/1‑165 (MQ=255)
                                                                                        cTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGCGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCgagaa                                                                                                                                                       >  1:302875/1‑208 (MQ=11)
                                                                                        cTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACAt                                                                                                                              >  2:913159/1‑234 (MQ=255)
                                                                                             cGCCTCGCCAAGCGAGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACgg                                                                                                                                                                                  <  2:286413/177‑1 (MQ=255)
                                                                                                                              cACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGa                                                                                                                                  <  2:505953/192‑1 (MQ=255)
                                                                                                                               aCCTACCCCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGAc                                                                                                             <  1:230092/212‑1 (MQ=255)
                                                                                                                                     acCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCa                                                                                                                                                                                                     <  2:903530/118‑1 (MQ=40)
                                                                                                                                          gACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGGCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTc                                                                                                                                                                               >  2:106605/1‑135 (MQ=255)
                                                                                                                                            tccTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGCAACTTcc                                                                                                                                   >  2:328866/2‑176 (MQ=255)
                                                                                                                                            cccTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGACCCCAGCCTCGACTGt                                                                                              >  2:485988/1‑214 (MQ=255)
                                                                                                                                            cccTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCgg                                                                                                                                                                                                                                            <  2:830319/72‑1 (MQ=39)
                                                                                                                                            cccTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCa                                                                                                                                                                       <  2:953645/141‑1 (MQ=255)
                                                                                                                                            cccTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCa                                                                                                                                                                       >  1:953648/1‑141 (MQ=255)
                                                                                                                                            cccTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGaa                                                                                                                                 >  2:230089/1‑179 (MQ=39)
                                                                                                                                            cccTGAGCAACCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTCCCGTTGAAGCGGGTAAAACCCAGCGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGACCCCAGCCTCGACTGTCATCAATGACAGCATCGATAc                                                                         <  1:161168/235‑1 (MQ=255)
                                                                                                                                              cTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGACCCCAGCCTCGACTGTCATCAATGACAGCAt                                                                               >  1:826251/1‑227 (MQ=255)
                                                                                                                                                    aaTCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGACCCCAGCCTCGAc                                                                                                 >  2:357548/1‑203 (MQ=255)
                                                                                                                                                         tGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGACCCCAGCCTc                                                                                                    >  2:836183/1‑195 (MQ=255)
                                                                                                                                                         tGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGACCCCAGCCTc                                                                                                    <  1:836186/195‑1 (MQ=255)
                                                                                                                                                           cccAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGACCCCAGCCTCGACTGTCATCAATGACAGCATCGATACCGTCACCGTAAGTAt                                                          <  1:681203/235‑1 (MQ=255)
                                                                                                                                                            ccAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCgg                                                                                                                                                                                                                                            <  1:679705/56‑1 (MQ=255)
                                                                                                                                                                   ccGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACCACAACCGCTccgccc                                                                                                                                                                          >  2:288911/1‑110 (MQ=255)
                                                                                                                                                                   ccGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCCGCGTCACCATc                                                                                                                                                            >  2:374800/1‑129 (MQ=38)
                                                                                                                                                                   ccGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGACCCCAGCCTCGACTGTCATCAATGACAGCATCGATAc                                                                         >  2:858610/1‑212 (MQ=255)
                                                                                                                                                                     ggTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCg                                                                                                               <  2:852614/172‑1 (MQ=255)
                                                                                                                                                                     ggTAACCGTGACCTTGTCCAACGGCCAAACCATAACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGACCCCAGCCTCGACTGTCATCAATGACAGCATCGATACCGTCACCGTAAg                                                             <  2:403747/222‑1 (MQ=255)
                                                                                                                                                                       tAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGACCCCAGCCTCGACTGTCATCAATGACAGCATCGATAc                                                                         <  2:704239/208‑1 (MQ=255)
                                                                                                                                                                        aaCCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAGACCCAGGACAGCGTCGATTGCCACACCCCTGCCAATGAAGTCTACAACAACGGCGCAAcc                                                                                                                                                                           >  2:847040/1‑109 (MQ=14)
                                                                                                                                                                               aCCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGACCCCAGCCTCGACTGTCATCAAt                                                                                       >  2:744215/1‑186 (MQ=255)
                                                                                                                                                                                       cAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGACCCCAGCCTCGACTGTCATCAATGACAGCATCGATACCGTCACCGTAAGTATTGTCAGCAATGGCAATGTGACCGAAGAc                              <  1:485991/235‑1 (MQ=255)
                                                                                                                                                                                             ccAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCCCGACCGTCAGCGTCACCATCGAGATCGCCACGGGCGGCAAGTTCGa                                                                                                                                  >  1:99835/1‑129 (MQ=255)
                                                                                                                                                                                              cAAACCATTACCGTTGAAGCCGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGACCCCAGCCTCGACTGTCATCAATGACAGCATCGATACCGTCACCGTAAGTa                                                           <  2:707222/199‑1 (MQ=255)
                                                                                                                                                                                                  ccATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGCAACTTCGAACAACTGACCCCGAATCCGACCCCAGCCTCGACTGCCATCAATGACAGCATCg                                                                             >  2:979204/1‑177 (MQ=11)
                                                                                                                                                                                                       aCCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGACCCCAGCCTCGa                                                                                                  <  2:109450/151‑1 (MQ=255)
                                                                                                                                                                                                         cGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGACCCCAGCCTCGa                                                                                                  <  1:95083/149‑1 (MQ=255)
                                                                                                                                                                                                         cGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGACCCCAGCCTCGa                                                                                                  >  2:95081/1‑149 (MQ=255)
                                                                                                                                                                                                         cGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGACCCCAGCCTCGACTGTCATCAATGACAGCATCGATACCGTCACCGTAAGTATTGTCAgct                                                  >  2:767616/1‑196 (MQ=255)
                                                                                                                                                                                                         cGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGACCCCAGCCTCGACTGTCATCAATGACAGCATCGATACCGTCACCGTAAGTATTGTCAGCAATGGCAATGTGACCGAAGACcagca                         <  2:926484/222‑1 (MQ=255)
                                                                                                                                                                                                          gTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCCCCTGCGGCCACTTcccacag                                                                                                                              >  1:324149/1‑114 (MQ=1)
                                                                                                                                                                                                              aaGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGACCCCAGCCTCGACTGTCATCAATGACAGCATCGATACCGTCACCGTAAGTATTGTCAGCAATGGCAATGTGACCGAAGACCAGCAGCCTTCCTTTACTGTGaa       >  1:550489/1‑235 (MQ=255)
                                                                                                                                                                                                              aaGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGACCCCAGCCTCGACTGTCATCAATGACAGCATCGATACCGTCACCGTAAGTATTg                                                        >  2:693898/1‑186 (MQ=255)
                                                                                                                                                                                                                gCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGACCCCAGCCTCGACTGTCATCAATGACAGCATCGATAcc                                                                        <  2:750381/168‑1 (MQ=255)
                                                                                                                                                                                                                gCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGACCCCAGCCTCGACTGTCATCAATGACAGCATCGATAcc                                                                        >  1:750384/1‑168 (MQ=255)
                                                                                                                                                                                                                  gggTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGACCCCAGCCTCGACTGTCATCAATGACAGCATCGATACCGTCACCGTAAGTATTGTCAGCAATGGCAATGTGACCGAAGACCAGCAGCCTTCCTTTACTGTGAAAGTaa  <  1:630970/236‑1 (MQ=255)
                                                                                                                                                                                                                  |                                                                                                                                                                                                                                           
CACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGACCCCAGCCTCGACTGTCATCAATGACAGCATCGATACCGTCACCGTAAGTATTGTCAGCAATGGCAATGTGACCGAAGACCAGCAGCCTTCCTTTACTGTGAAAGTAA  >  AE015451/197656‑198101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑