| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 199,360 | T→C | 9.4% | A1622A (GCT→GCC) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 199,360 | 0 | T | C | 9.4% | 266.0 / 6.5 | 90 | A1622A (GCT→GCC) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base T (37/44); new base C (4/5); total (41/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTG > AE015451/199130‑199580 | gtcgtcGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCa < 2:1003174/236‑1 (MQ=35) gtcgtGGCAGCCAACAGCACCACCGGTTCGGCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGa > 1:644374/1‑235 (MQ=35) caccGGTTCGGCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTc < 1:554466/235‑1 (MQ=35) gTTCGGCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAAc > 1:50252/1‑227 (MQ=17) cTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCaa < 1:220497/223‑1 (MQ=35) aGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAAc > 1:332731/1‑214 (MQ=35) aGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGGCAAGc > 2:634900/1‑221 (MQ=37) cccGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCa < 1:469433/236‑1 (MQ=255) ggACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGc < 1:1061256/198‑1 (MQ=18) gACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAg > 2:1035148/1‑186 (MQ=38) gACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTg > 1:1059248/1‑225 (MQ=40) gACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAg > 2:854159/1‑186 (MQ=18) cAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGCCAACCAGc > 1:1069871/1‑236 (MQ=255) aCGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGcc < 1:50006/235‑1 (MQ=255) cTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTg < 1:504641/235‑1 (MQ=255) cTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTg > 1:1087521/1‑235 (MQ=255) tCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTc < 1:250418/194‑1 (MQ=37) cGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTc < 2:405274/193‑1 (MQ=37) ggCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACg < 1:712006/235‑1 (MQ=255) ggCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACg < 1:621608/235‑1 (MQ=255) accaaccaGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGc > 1:818334/1‑193 (MQ=21) accaaccaGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGAc > 2:684465/1‑165 (MQ=38) accaaccaGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCt > 1:925773/1‑236 (MQ=255) accaaccaGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCCGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTc > 2:170352/1‑173 (MQ=38) ccaaccaGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCAc < 1:519137/209‑1 (MQ=255) ccaaccaGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCAc > 2:519134/1‑209 (MQ=40) ccaaccaGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCaa > 2:970983/1‑214 (MQ=255) caaccaGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCg > 2:381326/1‑203 (MQ=40) caaccaGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTgg > 1:424544/1‑236 (MQ=255) aaccaGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGt < 1:731633/236‑1 (MQ=255) aaccaGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTc < 1:854162/186‑1 (MQ=18) caGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGGCg < 1:1074385/235‑1 (MQ=39) aGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCGTCACCGTGc > 2:294159/1‑201 (MQ=255) ggtgggCAATGCCAGCGACGCGGTCAGCGGCGCAGATGCAGGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTc < 2:50251/178‑1 (MQ=255) tCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACg > 2:478605/1‑216 (MQ=39) tCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACg < 1:478608/216‑1 (MQ=40) gCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGCCATCGTCAAGGTCACCATCACGGCTGACCAGACTTCgg > 2:305767/1‑154 (MQ=25) gCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGca < 2:1087518/232‑1 (MQ=38) ccATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTg > 2:1037700/1‑221 (MQ=21) aCGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCt > 2:330241/1‑208 (MQ=38) aCGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCt > 2:1011824/1‑208 (MQ=38) cgGTCAGCGGCGCAGATGCATGGAAGTTCTAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCATGTCAACCAGCCGCTGGCCCACGACCTGGTCGTg < 2:1028075/212‑1 (MQ=9) cggcgCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGTACGCCAGGCAACGAAGGCGATATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCa < 1:1027599/236‑1 (MQ=255) cggcgCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCa < 1:381329/236‑1 (MQ=40) tGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGcc < 2:1066872/174‑1 (MQ=40) cATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGcaacaatg > 2:381415/1‑208 (MQ=18) aaGTTCGAAAACCTGAACCTGGCCAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCa < 2:504462/235‑1 (MQ=255) cGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACGCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGc > 2:179656/1‑161 (MQ=37) aaaCCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGcaaca < 2:468268/194‑1 (MQ=35) aaaCCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGcaaca > 1:468271/1‑194 (MQ=35) acctgaacctgGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGt < 2:925770/234‑1 (MQ=255) aGACCCCGGTCACCACCGAGGTCACCGACGAGCCAGGAACTCCAGGCAACGAAGGTGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGATc < 2:577252/161‑1 (MQ=255) cccGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAg > 1:40451/1‑233 (MQ=17) cccGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGCCACCATCACGGCTGACCAg > 2:79395/1‑85 (MQ=18) cGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGCCGCGCAAGGCGAt < 1:661305/236‑1 (MQ=255) cGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCg < 2:424541/234‑1 (MQ=255) ggTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTg < 1:485289/157‑1 (MQ=35) tCAGCACCGAGGTCACCGACGAGCCAGGTACGCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCa < 2:969897/126‑1 (MQ=255) cGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGCCAAGGTCACCATCACGGCTGACCAGACTGCGGTAGCCGAGCACGCCAAGCCGACCATCACCGTg > 2:1046225/1‑112 (MQ=14) ggTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGCCATCGTCACGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAgcg < 1:114837/194‑1 (MQ=255) ggcACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGCCATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAgg < 1:298282/233‑1 (MQ=255) gTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAgg < 1:768889/235‑1 (MQ=255) tCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTgg < 2:405753/181‑1 (MQ=40) aCCGACGTGCCAAGCAGTCCAGGGGACGAAGGGGAGTTCGTAAAGGACAGCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGa < 1:876585/94‑1 (MQ=14) gacgaGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTgg < 1:179658/176‑1 (MQ=39) gacgaGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCa < 1:526332/184‑1 (MQ=255) gacgaGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCa > 2:526329/1‑184 (MQ=39) gacgaGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCt < 1:842321/234‑1 (MQ=255) gacgaGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCAACCTTCACCGTGCACGTCAACACGCCGTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTACAcc > 1:163372/1‑198 (MQ=255) ccAGGCACTCCAGGCAACGAAGGCGACATCGTCAACGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCa < 2:432584/163‑1 (MQ=39) cAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCa < 2:818331/162‑1 (MQ=39) tacgccAGGCAACGAAGGCGATATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAg > 2:1023679/5‑135 (MQ=255) cacgccAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAg < 1:1023682/131‑1 (MQ=255) tCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACg < 1:691911/113‑1 (MQ=40) gccAGGCAACGAAGGCGATATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACg > 2:691908/2‑113 (MQ=255) ggCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGcaaca > 2:1055736/1‑133 (MQ=38) gagAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGccc < 1:170334/133‑1 (MQ=39) ggCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTggcg > 1:540490/1‑149 (MQ=39) ggCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAgg < 2:451727/185‑1 (MQ=40) ggCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAgg > 1:451730/1‑185 (MQ=255) ggCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGAc > 1:172765/1‑204 (MQ=255) ggCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAGACCAGCGCGCCGTACACCCacg > 2:779194/1‑175 (MQ=255) cATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCt < 2:388808/62‑1 (MQ=255) cATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCt > 1:388811/1‑62 (MQ=255) cATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTg < 1:693682/235‑1 (MQ=255) gTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGca < 2:565253/112‑1 (MQ=255) gTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGca > 1:565256/1‑112 (MQ=255) gTCACCATCACGGCTGCCCAGACTTCGGTAGCCGAGACCGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTg < 1:287391/235‑1 (MQ=255) aTCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACg > 2:439659/1‑172 (MQ=16) aCGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGc < 1:55083/122‑1 (MQ=40) | GTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTG > AE015451/199130‑199580 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |