| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 199,477 | T→C | 6.6% | A1661A (GCT→GCC) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 199,477 | 0 | T | C | 6.6% | 227.7 / 2.5 | 69 | A1661A (GCT→GCC) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base T (28/36); new base C (2/3); total (30/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCAC > AE015451/199253‑199701 | aaGTTCGAAAACCTGAACCTGGCCAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCa < 2:504462/235‑1 (MQ=255) acctgaacctgGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGt < 2:925770/234‑1 (MQ=255) cccGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAg > 1:40451/1‑233 (MQ=17) cGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGCCGCGCAAGGCGAt < 1:661305/236‑1 (MQ=255) cGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCg < 2:424541/234‑1 (MQ=255) ggTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGCCATCGTCACGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAgcg < 1:114837/194‑1 (MQ=255) ggcACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGCCATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAgg < 1:298282/233‑1 (MQ=255) gTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAgg < 1:768889/235‑1 (MQ=255) tCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTgg < 2:405753/181‑1 (MQ=40) gacgaGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTgg < 1:179658/176‑1 (MQ=39) gacgaGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCa < 1:526332/184‑1 (MQ=255) gacgaGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCa > 2:526329/1‑184 (MQ=39) gacgaGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCt < 1:842321/234‑1 (MQ=255) gacgaGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCAACCTTCACCGTGCACGTCAACACGCCGTTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTACAcc > 1:163372/1‑198 (MQ=255) ggCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTggcg > 1:540490/1‑149 (MQ=39) ggCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAgg < 2:451727/185‑1 (MQ=40) ggCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAgg > 1:451730/1‑185 (MQ=255) ggCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGAc > 1:172765/1‑204 (MQ=255) ggCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAGACCAGCGCGCCGTACACCCacg > 2:779194/1‑175 (MQ=255) cATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTg < 1:693682/235‑1 (MQ=255) gTCACCATCACGGCTGCCCAGACTTCGGTAGCCGAGACCGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTg < 1:287391/235‑1 (MQ=255) aTCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACg > 2:439659/1‑172 (MQ=16) aCCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGcc > 2:813940/1‑180 (MQ=255) agagacagcGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAAc < 1:291595/227‑1 (MQ=255) cAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCGGTAGACGCCACTGGtgcgg > 2:658734/1‑221 (MQ=255) agaACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACGCCCACGACGCGCAAGGAGATGACGGCTATCAGGACGCTGGCCAGATCCGc > 2:250454/1‑170 (MQ=255) aaCGCCAAGCCGTCCGTCACAGTGCACGTCAACCCGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGCCGCGCAAGGCGATGACGTCTATCAGGACGCTGGcc < 1:1076592/160‑1 (MQ=14) gTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTcggcggag > 2:291592/1‑184 (MQ=255) gTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCt < 2:644371/221‑1 (MQ=255) tCAAGCCGCCCTTCACCGGGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGc < 2:350310/190‑1 (MQ=255) gccgccCTTCCCCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTggg < 2:332728/165‑1 (MQ=255) gCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGtgc > 2:772219/1‑225 (MQ=255) gCCGACCTCCCCCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCgaga < 1:168971/206‑1 (MQ=255) tCACCTTGCACGACAACCAGCCGCTAGACCACGACCTGCTCCAGCACCTGCACAACAGCACCAAGGTCACCATCAAGGCTGGAGAGACCAGCGCGCCGTACAcc < 2:512480/104‑1 (MQ=2) tCACCGTGCACGGCAACCAGCCGCTGGCCCACGA‑CTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTcggcg < 1:93448/169‑1 (MQ=255) aCGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTc > 1:663395/1‑236 (MQ=255) gTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGA‑CAGCGCGCCGTACACCCACGAcgcg > 2:784660/1‑101 (MQ=255) gTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGA‑CAGCGCGCCGTACACCCACGAcgcg < 1:784663/101‑1 (MQ=255) aaCCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCGcgac > 1:888748/1‑234 (MQ=255) ccAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCa < 2:172763/134‑1 (MQ=37) gCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAgcg > 2:422565/1‑74 (MQ=34) gCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAgtg > 1:1038683/1‑235 (MQ=255) cGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTcgacg > 2:1058908/1‑228 (MQ=255) cGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAgtggtg < 1:772222/236‑1 (MQ=255) ggCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCa > 1:509162/1‑236 (MQ=255) cTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGAc < 2:1038680/233‑1 (MQ=255) cGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCAccc > 1:93471/1‑236 (MQ=255) ccTGAGCAACAACGCCCAGGTCGCCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGtgc < 2:113155/141‑1 (MQ=37) cTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTAt > 2:734195/1‑84 (MQ=255) cTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTcggca > 1:547673/1‑122 (MQ=255) cTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTcggca < 2:547670/123‑2 (MQ=255) cTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTcggca > 1:600939/1‑122 (MQ=255) gCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGAcc > 1:917690/1‑236 (MQ=255) cGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTAGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCCGACTGCCACCCCTTCGGTGACCGAAg < 2:663392/233‑1 (MQ=255) cGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTgccgct > 2:688123/1‑163 (MQ=255) gCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCgg > 1:897261/1‑223 (MQ=255) ccAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGt < 1:170354/235‑1 (MQ=255) ccAGGTCACCATCAAGGCTGGCGAGACCAGCGCACCGTACACTCACGACGCGCAAGGCGATGACGTCTATCAGGACGCGGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGt < 1:391404/235‑1 (MQ=255) ccAGGTCACCATCAAGGACGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGt < 1:305770/235‑1 (MQ=255) aGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTgag > 1:44291/1‑236 (MQ=255) gTCACCATCAAGGCCGGCGAAACCAGCGCACCGTACACTCACGACGCGCAGGGCGATGACGTCTATCAGGATGCGGGCCAGATCAGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCaa < 2:857172/197‑1 (MQ=11) tCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGGGGACGCCACTGGtgctg > 2:124264/1‑123 (MQ=25) aCCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGCCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCa < 1:527893/235‑1 (MQ=255) ccATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCAc < 1:970986/235‑1 (MQ=255) ccATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCAc < 1:400430/235‑1 (MQ=255) cATCAAGGCCGGCGAAACCAGCGCACCGTACACTCACGACGCGCAGGGCGATGACGTCTATCAGGATGCGGGCCAGATCAGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCAc < 2:740063/234‑1 (MQ=11) aTCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTg < 1:517182/226‑1 (MQ=255) aTCAAGGCCGGCGAAACCAGCGCACCGTACACTCACGACGCGCAGGGCGATGACGTCTATCAGGACGCGGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGtct > 2:391401/1‑155 (MQ=11) ggCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCAc < 1:330244/228‑1 (MQ=255) | AAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCTTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCTGCATTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCAC > AE015451/199253‑199701 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |