| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 200,338 | G→C | 7.0% | V1948V (GTG→GTC) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 200,338 | 0 | G | C | 7.0% | 216.3 / 6.9 | 68 | V1948V (GTG→GTC) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base G (26/37); new base C (3/2); total (29/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01 | |||||||||||
GCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCGGT > AE015451/200106‑200547 | gCAACAGTGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCGTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCCACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCAGTCAcc < 1:1048288/236‑1 (MQ=14) cgcgccctcaacggttgcgatcgacatcacgacagggtcgttagcgccgacgtagacgttgtccggagcagtcacggtggcagtgcccacggtgccgttggccggcacagtgatgacggttttgccgtcgctcagcgtgaaggtcaatgcactgtggttgttgatcggcagaccgtctttgttggtcagcgtgatggtgtaagtgatctcaccgCCTTCGGTCACCGAAGGCGGTg > 1:1080262‑M1/215‑236 (MQ=255) tCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATc < 1:168383/236‑1 (MQ=255) aCCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCAc < 1:621934/236‑1 (MQ=255) ccATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCAc < 1:578039/235‑1 (MQ=255) tGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATc < 1:940820/235‑1 (MQ=255) gAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCAccc > 1:407884/1‑235 (MQ=255) tACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGTCAGTAGACGCCACTGGTGATGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCAc < 1:1059220/206‑1 (MQ=255) cacCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTc < 1:779197/236‑1 (MQ=37) ccACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGt < 2:441481/193‑1 (MQ=255) cgcAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATcaa > 1:594235/1‑235 (MQ=40) gcAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCAccc < 1:438985/206‑1 (MQ=40) gCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACg < 1:209249/216‑1 (MQ=39) gCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCAc < 1:605921/236‑1 (MQ=255) gACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCg < 1:350084/219‑1 (MQ=39) tATCCGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCt < 1:61396/209‑1 (MQ=255) tCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACACTCGACGAAGTAGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCAACAACATCAc > 1:14346/1‑186 (MQ=255) aGGACGCTGGCCAGATCAGCCTGGGCATCAGCTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTc < 1:1083032/236‑1 (MQ=255) cggtggcagtgcccacggtgccgttggccggcacagtgatgacggttttgccgtcgctcagcgtgaaggtcaatgcactgtggttgttgatcggcagaccgtctttgttggtcagcgtgatggtgtaggtgatctcgccgCCTTCGGTGACCGAAgg < 2:593264‑M1/17‑1 (MQ=255) tGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATcaacaa > 2:725069/1‑210 (MQ=255) gCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATcaa < 1:896280/205‑1 (MQ=255) gCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATcaa > 2:896277/1‑205 (MQ=40) ccAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGAcc > 1:78004/1‑235 (MQ=40) aGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACg < 2:866430/189‑1 (MQ=255) aGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGaa > 2:1083029/1‑198 (MQ=255) tCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACgg < 1:982254/235‑1 (MQ=40) gggCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCAGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAAcc > 2:801388/1‑197 (MQ=14) gggCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCAGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAAcc < 1:801391/197‑1 (MQ=14) aTCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACcgtcgt > 1:367797/1‑236 (MQ=255) cTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTCACCGAAGGCGGTg > 2:14537/1‑134 (MQ=255) tCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCt > 1:229785/1‑197 (MQ=40) cggTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGGCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCAc < 1:1024124/134‑1 (MQ=255) aGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGt < 1:935576/194‑1 (MQ=39) aGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGcc > 1:635535/1‑235 (MQ=40) gTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAAcc < 1:688126/168‑1 (MQ=39) tgctgcATTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATc < 1:887554/130‑1 (MQ=17) ttCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCT‑CACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACTCGGAGc > 2:3669/1‑168 (MQ=255) gagaACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAg > 2:775181/1‑177 (MQ=35) gaACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGtt > 2:372765/1‑224 (MQ=39) gAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCAcc < 1:906162/236‑1 (MQ=255) cTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCCACTGCAACCGCACCa < 2:635532/234‑1 (MQ=14) cTGGGCGGTGCTGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCAcc < 2:426070/233‑1 (MQ=25) tGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCACCa < 2:594232/233‑1 (MQ=255) gcactgtggttgttgatcggcagaccatctttgttggtcagggtgatggtgtaggtgatctcaccaCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGCTGGTCTGCCGATCAACAACCAc < 1:294872‑M1/78‑1 (MQ=255) ggttattgatcggcaagccgtctttgttggtcagggtaatggtgtaagtgatctcaccgCCTTCGGTGACCGAAGGCGGTGAGATCACTTATACCATTACCCTGACCAACAAAGACg < 2:912280‑M1/58‑1 (MQ=255) cGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACTCGGAGCTGTCCTTCAAGCTGACCGACGGCACCACtgtcgt > 2:174132/1‑177 (MQ=2) cGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCACCAGACAACGTCTATg > 1:927612/1‑236 (MQ=37) cTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGt < 2:888745/144‑1 (MQ=32) tCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCACCAGACAACGTCTATGt < 2:407881/233‑1 (MQ=38) cAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCACCAGACAACGCCTAt > 1:107323/1‑225 (MQ=37) aCCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACgcgc < 1:658737/235‑1 (MQ=38) cacCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGt < 1:509190/181‑1 (MQ=37) cacCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGt > 2:509187/1‑181 (MQ=35) ggttggtcagggtaatggtgtaagtgatctcaccgCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACTACTGTCGTCGTGGCAGCCAACAGca > 1:451587‑M1/36‑171 (MQ=255) ccTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCggt < 2:927609/231‑1 (MQ=38) cgacgaAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGCTCGGCCACTGCAACCGCACCAg > 2:1067893/1‑197 (MQ=14) cgacgaAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACGGGtt > 2:798824/1‑176 (MQ=17) aaGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCAcc < 2:78002/190‑1 (MQ=39) gtggCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGc > 2:820581/1‑227 (MQ=39) gtggCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCACCAGACAACGTCCATGTCGGCACCAACGCGCCg > 2:532125/1‑216 (MQ=39) gtggCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCAGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGCTCTGCCCATCAACAACCACTc > 2:64252/1‑101 (MQ=255) ggCCAAGCTCACTCCCACCCCTTCGGTGACCGAAGGCGGTGAGCTCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCg < 1:124266/230‑1 (MQ=255) aggtgatctcaccgCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCACCAGACAACGCCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATc < 1:454162‑M1/210‑1 (MQ=255) aCCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCGGt < 2:405995/221‑1 (MQ=25) CCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGAt > 2:1042113‑M1/1‑68 (MQ=255) CCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGAt < 1:1042116‑M1/68‑1 (MQ=255) TTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATg > 1:728956‑M1/1‑203 (MQ=255) CGGTGACCGAAGGCGGTGAGATCACCTACAGCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGa > 2:31616‑M1/1‑79 (MQ=255) | GCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCGGT > AE015451/200106‑200547 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |