| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 205,795 | T→A | 7.2% | A3767A (GCT→GCA) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 205,795 | 0 | T | A | 7.2% | 206.8 / 8.8 | 69 | A3767A (GCT→GCA) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base T (33/31); new base A (2/3); total (35/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.16e-01 | |||||||||||
GCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACAGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGATCTTCA > AE015451/205581‑206030 | gcAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACaa > 1:1027077/1‑235 (MQ=35) cGCTTCGGGTCAAGTGCCCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCt < 1:173076/236‑1 (MQ=18) aaGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTAc < 1:1064534/235‑1 (MQ=37) gACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGAc < 2:545736/234‑1 (MQ=37) caccacCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACCGCCCCCGACac > 2:200155/1‑209 (MQ=21) tggtggCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACTAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAatcgatc < 1:601429/236‑1 (MQ=39) tgggggCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGAGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCg < 2:71865/191‑1 (MQ=16) gtggCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAatcgatc < 2:391379/234‑1 (MQ=39) aaGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCtgg > 1:234464/1‑214 (MQ=25) cgcgACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCg < 1:777026/175‑1 (MQ=34) cgACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCCCTGCCCCGGACCACGTCTACGTCGGTACCAACGCCCCtg > 1:6822/1‑204 (MQ=21) cGGTGACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGACACCGTGCGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGt > 1:719475/1‑178 (MQ=16) cGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGa < 2:476136/190‑1 (MQ=35) cGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGa > 1:476139/1‑190 (MQ=35) cGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCtg > 2:896325/1‑195 (MQ=34) cGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCg > 2:547801/1‑234 (MQ=37) aGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGc < 1:896328/236‑1 (MQ=37) ttACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTTGGCACCa < 1:94948/161‑1 (MQ=11) cccccTGACCAACAACGACGGCTTGCCGATCAACCACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAACCCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAatcga < 1:213722/169‑1 (MQ=11) cGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGTACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCtgt > 2:509214/1‑160 (MQ=18) cGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCgg < 2:445016/192‑1 (MQ=37) cGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTc > 2:698186/1‑206 (MQ=38) ccAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCa > 2:376405/1‑234 (MQ=37) aacaaAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAatcgat > 2:895632/1‑164 (MQ=35) cGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGAc < 1:94934/115‑1 (MQ=32) cGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGAc > 2:94932/1‑115 (MQ=32) gTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGCCAAGACGCCGGTCAGCACGt > 1:976326/1‑225 (MQ=25) gTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGcc > 1:575232/1‑214 (MQ=34) tGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTGATCAAGTCGATCGCAACCGTTGGAGGTGCGGACGTCGGCAAGTTCGAGCaa > 1:175555/1‑191 (MQ=21) gCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGTGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGt > 1:735501/1‑159 (MQ=14) cGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCa > 1:1004945/1‑236 (MQ=255) aTCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACCCCGGTCAGCACGTCGGTCACCGACGAGccg < 2:700522/234‑2 (MQ=255) cATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCa > 2:612534/1‑231 (MQ=255) cATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGACCCa > 1:648531/1‑218 (MQ=255) gACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAgg < 2:448034/234‑1 (MQ=255) ccTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGt > 1:290755/1‑215 (MQ=255) cTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGc < 2:838610/232‑1 (MQ=255) aCGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCTATCGCAACCGTTTAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGc > 1:79833/1‑166 (MQ=25) aCGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGa < 2:776374/224‑1 (MQ=255) aCGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGa > 1:514844/1‑230 (MQ=255) aCGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGt > 1:465135/1‑236 (MQ=255) aCGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACCGCACCGTGGGCACTGCTCCCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACg < 1:193852/223‑1 (MQ=255) cGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTc < 2:1004942/236‑1 (MQ=255) gCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTgg > 2:599671/1‑167 (MQ=38) gCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTgg < 1:599674/167‑1 (MQ=37) gCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGc > 2:385108/1‑226 (MQ=255) gCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGc < 1:385111/226‑1 (MQ=255) gCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGGACGCCGGGCa > 2:920750/1‑218 (MQ=255) tGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGc < 1:52928/182‑1 (MQ=35) ggCAAACCCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGt < 2:22183/198‑1 (MQ=255) aCCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAg < 2:94649/156‑1 (MQ=35) cGTCATCACCGCGCCGGCCAACGCCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACAGc < 2:475438/234‑1 (MQ=255) tcaCCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACcgac < 1:1088821/176‑1 (MQ=255) ccGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGc < 2:211489/200‑1 (MQ=255) gCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCAt > 2:885947/1‑216 (MQ=255) ccGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACcga > 2:480138/1‑167 (MQ=37) ccGGCCAACGGCACCGTGGGCACTGCAACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACcga < 1:480141/167‑1 (MQ=255) cGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTg > 1:757734/1‑200 (MQ=255) cGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACAGCCGACCAGACTTc > 2:478845/1‑232 (MQ=255) gCCAACGGCACCGTGCGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACAGCCGACCAGACTTCGGTGGc < 1:341175/236‑1 (MQ=255) cACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACCCCGGTCAGCActt > 2:519170/1‑144 (MQ=25) cGTGGGCCCTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACAGCCGACCAGACTTCGGTGGCCGAGAACGTc < 1:200157/235‑1 (MQ=255) cGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACAGCCGACCAGACTTCGGTGGCCGAGAACGTc < 1:376408/235‑1 (MQ=255) tGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACAGCCGACCAGACTTCGGTGGc < 2:375443/223‑1 (MQ=255) gggCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACAGCCGACCAGACTTCGGTGGCCGAGAACGTCaa > 2:1059275/1‑234 (MQ=255) gCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCCAGGTCACCATCACAGCCGACCAGACTTCGGTGGCCGAGAACGTc > 1:306566/1‑230 (MQ=255) gCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAAGCGACCTGGTCaa > 2:889550/1‑187 (MQ=255) cACGGCTACCGTCACAGCCCCGGACAACGTCTACGTCGGTACCAACGCGCCTGTGATCAAAACGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTc < 2:145837/130‑1 (MQ=11) aCTGCTACCGTCGCTGCCCCGGACATCGTCTACGTCGGTACCAACGATCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACg < 1:126915/159‑1 (MQ=255) tACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACAGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGATCTTCa < 1:895635/236‑1 (MQ=40) | GCAACGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACAGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGATCTTCA > AE015451/205581‑206030 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |