| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 213,079 | C→T | 8.4% | G6195G (GGC→GGT) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 213,079 | 0 | C | T | 8.4% | 234.0 / 5.1 | 80 | G6195G (GGC→GGT) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base C (38/35); new base T (2/5); total (40/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
TTCGAGAACCTGGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTCGTGGCCAAGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGC > AE015451/212849‑213311 | ttCGAGAACCTGGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACCCCACCGACGAAGTCGTGGCCAAGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGc < 1:232979/236‑1 (MQ=37)ttCGAGAACCTGGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTCGTGGCCAAGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACt > 2:974506/1‑234 (MQ=39)ttCGAGAACCTGGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTCGTGGCCAAGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGc < 1:418662/236‑1 (MQ=37) agaACCTGGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTCGTGGCCAAGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGc < 1:1008640/227‑1 (MQ=38) cTGGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTCGTGGCCAAGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCg < 1:525304/232‑1 (MQ=38) cGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTCGTGGCCAAGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACa > 1:170825/1‑236 (MQ=40) ccGCGAAGGTGGATGTCACCGACACCACCGACGAAGTCGTGGCCAAGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTc < 1:974509/236‑1 (MQ=255) cgAAGGTGGATGTCACCGACACCACCGACGAAGTCGTGGCCAAGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTAc > 1:325236/1‑236 (MQ=255) aaGGTGGATGTCACCGACACCACCGACGAAGTCGTGGCCAAGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACg < 1:373299/235‑1 (MQ=255) aTGTCACCGACACCACCGACGAAGTCGTGGCCAAGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGc < 1:1073667/199‑1 (MQ=25) cccGACACCACCGACGAAGTCGTGGCCCAGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACg < 1:251066/222‑1 (MQ=255) acacCACCGACGAAGTCGTGGCCAAGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACt > 2:368656/1‑200 (MQ=14) caccacCGACGAAGTCGTGGCCAAGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTg > 1:749401/1‑236 (MQ=255) accaccGACGAAGACGTGGCAAAGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGa < 1:308851/230‑1 (MQ=255) cgacgaAGTCGTGGCCAAGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTg < 1:919200/235‑1 (MQ=255) aaGTCGTGGCCAAGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATg > 1:726372/1‑233 (MQ=255) gTGGCCAAGTTGACCGCGACCCCGTCGGTGACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGAt > 2:931965/1‑233 (MQ=17) gTGGCCAAGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGc < 1:641854/236‑1 (MQ=255) tGGCCAAGCTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACt < 1:225623/170‑1 (MQ=14) aaGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCCCCGTCCCTCCCCCGGACAATGt > 1:1074472/1‑191 (MQ=255) gACCGCCACCCCGTCGGTCACCGAGGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAAt > 2:290415/1‑184 (MQ=255) cccGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAg < 1:205057/234‑1 (MQ=40) aCCGCCACCCCGTCGGTCACCGAAGACGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCg < 1:751711/166‑1 (MQ=25) cGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACCCCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCg < 2:214680/164‑1 (MQ=25) cGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGGCCCTGTCGTGATGTc < 1:334014/216‑1 (MQ=39) cACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTg > 2:961158/1‑167 (MQ=39) cACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTg < 1:961161/167‑1 (MQ=39) cACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACACTGTCCACGTCg > 2:213788/1‑176 (MQ=255) gAAGGCGGCGAGATTCCCTACACCAGCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAAt < 2:301411/162‑1 (MQ=37) gcgAGATTACCTACACCATCACGCTGACGAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGCCCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTg < 1:1047413/236‑1 (MQ=255) agaTTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGAc < 1:724553/235‑1 (MQ=38) agaTTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGa > 2:539936/1‑234 (MQ=40) aTTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTg > 2:652053/1‑231 (MQ=40) aTTACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGc < 1:1085684/226‑1 (MQ=39) cacCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGa < 1:840682/236‑1 (MQ=255) cATTACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCg < 1:773949/122‑1 (MQ=18) cccTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCaa > 1:563753/3‑151 (MQ=2) cccTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGCCACCGTGGGCACTGCCACCGTCTCTGCCCCGGACAATGTCTACGTCGGTACCat < 2:563750/149‑2 (MQ=11) cGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCaa > 1:1031828/1‑212 (MQ=39) cGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGACACTGCCCCGGACAATGTCTACGTCGGTACCAACGCCCCTg > 2:98562/1‑159 (MQ=25) gCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAATGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGCCAAGACGCCGGTc > 1:170659/1‑235 (MQ=255) gCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGAcac > 2:641805/1‑216 (MQ=38) aacaaAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAg > 2:934403/1‑195 (MQ=40) aacaaAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGc > 2:167164/1‑231 (MQ=255) aGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGc > 1:574989/1‑99 (MQ=33) aGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCg < 1:980528/104‑1 (MQ=33) gCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGAcac > 2:834393/1‑199 (MQ=38) cGATTAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAg < 1:432331/162‑1 (MQ=255) cGATTAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAg > 2:432328/1‑162 (MQ=255) cGATCAACATCCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCt < 1:1048423/134‑1 (MQ=21) aacCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTAcccc > 1:407248/1‑236 (MQ=16) tGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTggcc > 2:314572/1‑182 (MQ=255) tGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACg < 1:421167/236‑1 (MQ=255) ttCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGATCGAGCAACTGACGCTgg > 2:215798/1‑172 (MQ=255) cACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCg < 2:147680/155‑1 (MQ=255) aCGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCg > 2:862823/1‑192 (MQ=255) aCGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGt > 1:144544/1‑236 (MQ=255) gCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGc < 2:638234/226‑1 (MQ=255) gCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGc > 1:638237/1‑226 (MQ=255) gCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGCTGGCAAGCTCGAGCACCTGACGCTGGa > 2:309859/1‑168 (MQ=255) gACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGACGGCGCGGACGTTGGCAAGTTCGACCAACTGACTCTCGCCAAgccccc > 2:94472/1‑165 (MQ=11) aCGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGCACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACt < 2:941463/222‑1 (MQ=255) aaaaCCGTCATCACCGTGCCGGCTAATGGCACCGTGGGCACTGCCACTGTCACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTgg < 2:405591/220‑1 (MQ=255) aaaaCCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCa > 1:912810/1‑235 (MQ=255) aaCCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCg < 2:325233/190‑1 (MQ=255) ccGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCa < 1:950603/78‑1 (MQ=39) tcatcaCCGTGCCGGCTAATGGCACCGTGGGTACTGCCACCGTCACTGCCCCGGATAACGTCTACGTCGGTAGCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTgg < 1:967286/147‑1 (MQ=11) ccGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTGACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACTTCGGTCAcc < 2:191405/170‑1 (MQ=255) tGCCGGCAAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCt < 1:13372/234‑1 (MQ=255) ccGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGCACCCCAGGCAACGAAGGCGACTTGGTCAAGGGCACCATCACGGCCGACCAGACCTCGGt > 1:128319/1‑236 (MQ=255) cGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACACTGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCg > 2:162599/1‑173 (MQ=255) gCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAAcc < 1:533955/90‑1 (MQ=255) aaCGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCCACGACCCTGTCGTGCTGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCACGCTCGAGCAACTGACGCTGGACaa > 2:206486/1‑134 (MQ=255) aaCGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCg > 1:901422/1‑235 (MQ=255) cggTGGGCACTGCCACCGTCCCTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGc < 1:107521/157‑1 (MQ=255) ccGTGGGTACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGACCGCAACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACGc > 2:145960/1‑120 (MQ=255) ggTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGc > 2:162721/2‑181 (MQ=255) gTGGGTACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTAGCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTc < 2:454528/234‑1 (MQ=255) tGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGACAGCACGTCGGTGACcgacga > 2:497021/1‑154 (MQ=255) gggCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGCGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGc > 1:1028760/1‑236 (MQ=255) ggCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGAcc < 1:167166/213‑1 (MQ=255) | TTCGAGAACCTGGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTCGTGGCCAAGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACAATGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGC > AE015451/212849‑213311 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |