| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 215,377 | C→T | 6.0% | A6961A (GCC→GCT) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 215,377 | 0 | C | T | 6.0% | 229.2 / 4.0 | 81 | A6961A (GCC→GCT) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base C (33/43); new base T (3/2); total (36/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
CAAGTTCGAACAGCTTACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAGGTGGCGAGATCACTTACACCATC > AE015451/215152‑215611 | cAAGTTCGAACAGCTTACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCa < 1:20140/236‑1 (MQ=31) aGACGCCGGTCAGCACGTCGGTGACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCag > 1:371907/1‑235 (MQ=17) ccGGTCAGCACGTCGGTGACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCa < 1:800795/205‑1 (MQ=31) ccGGTCAGCACGTCGGTGACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGc > 2:458130/1‑234 (MQ=34) cGGTCAGCACGTCGGTGACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGAt < 1:970891/236‑1 (MQ=37) tCAGCACGTCGGTGACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGa > 2:992960/1‑196 (MQ=31) cGTCGGTGACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTa < 2:889272/235‑1 (MQ=39) gacgaGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAGGGCGATGACGTTTACAACGATGCt < 2:371904/234‑1 (MQ=9) gacgaGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTgcgc > 2:846425/1‑208 (MQ=32) acgaGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCa < 2:38962/183‑1 (MQ=31) gTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGGCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAGGGCGATGACGTTTACAACGATGc < 2:1021675/223‑1 (MQ=255) caggCAACGAAGGCGACTGGGTCAAGCTCCCCATCCCCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAgcgcg < 1:190987/171‑1 (MQ=9) aGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTcc < 1:631948/235‑1 (MQ=39) aGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTcc < 1:602847/235‑1 (MQ=37) gTCACCATCGCCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAacg < 2:108192/187‑1 (MQ=37) aCCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAACCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCCAGGCCGGCGAAccc < 1:819674/138‑1 (MQ=12) aTCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCtgt > 1:353466/1‑218 (MQ=38) tCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCa > 2:857734/1‑151 (MQ=34) cgccgACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCtt < 2:409004/234‑1 (MQ=37) cgccgACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTc < 1:640395/235‑1 (MQ=37) cgACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAGGGCGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGTATCAACTCCGCTGTAGac > 2:636519/1‑213 (MQ=255) cAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACAcc > 2:71765/1‑138 (MQ=34) gACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAg < 2:33132/178‑1 (MQ=38) aCCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGcag > 2:406618/1‑140 (MQ=21) aCCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCa > 1:738363/1‑236 (MQ=37) agaACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGa > 1:1006712/1‑103 (MQ=32) agaACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCtg > 2:26118/1‑189 (MQ=35) agaACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCACCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCt > 2:300608/1‑172 (MQ=25) gaACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCgg < 1:787165/236‑1 (MQ=34) aaCGTCGAGCCGACCTTCACCGTGCACGTCAACCCGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCg < 1:164565/229‑1 (MQ=21) gcGTCAAGCCGACCTTCACCGCGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCATCATCAAGGCCGGCGAAACCAGCGCGCCATCCACCCACGCTGCGCAAGGCg < 2:1006956/134‑1 (MQ=255) gTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAgcgcg > 1:553181/1‑108 (MQ=33) gTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAgcgcg < 2:553178/108‑1 (MQ=33) aaGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCCAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTg < 2:685131/219‑1 (MQ=21) cGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGtt < 2:934142/233‑1 (MQ=17) aCCGTCACCGTGCACGGCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATc < 1:745895/153‑1 (MQ=25) tCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGACACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGCCTACAacga > 2:1440/1‑136 (MQ=21) tCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCCGCTGTAGac > 2:440395/1‑175 (MQ=21) gccGCGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGc < 2:161294/116‑1 (MQ=25) aCCGTGCACGGCAACCGGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCa < 2:338554/88‑1 (MQ=255) gTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATac < 1:220119/236‑1 (MQ=32) cACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGc < 1:234453/144‑1 (MQ=34) aCGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGAt > 1:839236/1‑113 (MQ=255) aCGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGAt < 2:839233/113‑1 (MQ=18) aCGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATc < 2:1091055/152‑1 (MQ=33) aCGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCtt > 2:798333/1‑184 (MQ=33) aCGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATaccac > 1:925960/1‑235 (MQ=33) aCGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGGCTACAACGACGCTGGc > 2:550682/1‑134 (MQ=21) cGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATaccacc > 2:538566/1‑235 (MQ=33) gTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACcg < 1:837331/235‑1 (MQ=32) aaCCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCtt > 1:477796/1‑179 (MQ=34) aaCCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCtt < 2:477793/179‑1 (MQ=34) aaCCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGT‑‑‑GCTACCGTTCAAGTGACCGATACCACCGACGAagt > 1:567272/1‑236 (MQ=18) aaCCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGACCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTAc < 2:748295/209‑1 (MQ=17) tGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGcc < 1:84724/236‑1 (MQ=32) gCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTgg > 2:939982/1‑232 (MQ=33) gCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCa > 1:1071141/1‑235 (MQ=33) cccACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCaa < 1:1084116/235‑1 (MQ=33) ccTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCg > 1:84526/1‑86 (MQ=33) cTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACcg < 1:71767/235‑1 (MQ=32) gTCGTGACCCTGAGCAACAACGCCCAGGTCACCCTCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGAc < 1:118565/236‑1 (MQ=17) cGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTCGACGGTCGCACCtt > 2:317959/1‑153 (MQ=18) cGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGAcc < 1:538569/235‑1 (MQ=33) gtaagccTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGAccc > 1:581210/6‑235 (MQ=18) gTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGAccc > 1:71652/1‑235 (MQ=32) gTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCGGTGGATGTAGACGGCCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCCACCGGTCAAGCGACCGAGACCACCGACGAagga > 2:287389/1‑211 (MQ=255) tGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCACAGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCt < 1:600978/236‑1 (MQ=17) ccTGAGCAACACCGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCTTCg < 1:898333/235‑1 (MQ=17) gCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTa > 2:493071/1‑79 (MQ=21) gCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCt > 2:380754/1‑214 (MQ=33) caacaaCGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAag > 1:34661/1‑200 (MQ=33) cGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTctg < 2:37471/110‑3 (MQ=2) aGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGc < 2:562923/56‑1 (MQ=35) aCCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTg < 1:325327/141‑1 (MQ=32) aCCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTg > 2:325324/1‑141 (MQ=32) ccATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAGGTGGCGAGATCAc < 1:992963/235‑1 (MQ=31) aTCAAGGCTGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGaaa < 2:451276/127‑1 (MQ=9) aTCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCtt > 2:618298/1‑122 (MQ=33) aTCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCtt < 1:618301/122‑1 (MQ=32) aaGGCTGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACcg > 1:597178/1‑172 (MQ=12) aaGGCTGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACcg < 2:597175/172‑1 (MQ=14) ccGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGc < 1:572083/41‑1 (MQ=35) ccGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGTCCGCGACCCCTTCGGTCACCGAAGGTGGCGAGATc < 1:973506/224‑1 (MQ=14) ccGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGTCCGCGACCCCTTCGGTCACCGAAGGTGGCGAGATc > 2:973503/1‑224 (MQ=14) ccGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAGGTGGCGAGATCACTTACAcc > 2:820569/1‑233 (MQ=31) cGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAGGTGGCGAGATCACTTACACCATc < 1:594673/235‑1 (MQ=14) | CAAGTTCGAACAGCTTACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAGGTGGCGAGATCACTTACACCATC > AE015451/215152‑215611 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |