Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AE015451 3,845,703:1 +G 100% intergenic (‑70/+28) PP_3394 ← / ← PP_3395 putative 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/Transcriptional regulator, LysR family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE0154513,845,7031.G97.1% 119.4 / ‑2.8 34intergenic (‑70/+28)PP_3394/PP_3395putative 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/Transcriptional regulator, LysR family
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base G (16/17);  total (17/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.05e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CAGCAAGCGTGCCGGCACGAACGACGCGACTTCCATATGGCGCACGCCGGCGCCGTAAGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGC‑G‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAACGCCGCATAGCGGGACACGGCCTCTT  >  AE015451/3845475‑3845909
                                                                                                                                                                                                                                     |                                                                                                                                                                                                               
cagcaAGCGTGCGGGCACGAACGACGCGACTTCCATATGGCGCACGCCGGCGCCGTAAGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCAGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGAGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGC‑g‑ggctga                                                                                                                                                                                                         >  1:711125/1‑231 (MQ=255)
                                     tGGCGCACGCCGGCGCCGTAAGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGc                                                                                                                                                                     >  1:485921/1‑236 (MQ=255)
                                       gcgcACGCCGGCGCCGTAAGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGG‑GCTGATCCa                                                                                                                                                                                                      <  1:655854/201‑1 (MQ=255)
                                               cGGCGCCGTAAGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTg                                                                                                                                                           <  1:704800/236‑1 (MQ=255)
                                                     cGTAAGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGG‑GCTGATCCATCGTCAGcc                                                                                                                                                                                             <  2:130036/196‑1 (MQ=255)
                                                            ggcgTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAg                                                                                                                                              >  1:434068/1‑236 (MQ=255)
                                                              cgtcgtTCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCcgcg                                                                                                                                                                           >  1:170576/1‑205 (MQ=255)
                                                                  gATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCCGGCCGgcgccc                                                                                                                                                        >  2:106044/1‑218 (MQ=255)
                                                                           gcgTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAATCGTTGCGCCAGACTCAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAgg                                                                                                                                 <  2:405561/234‑1 (MQ=255)
                                                                                                                tGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCgcgc                                                                                             <  2:485920/233‑1 (MQ=255)
                                                                                                                   aaGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGCCGGGCAGAAAAGACGTGTTTCGCGG‑GCTGATCCATCGTCAGCCACGAt                                                                                                                                                                                        >  2:173465/1‑139 (MQ=255)
                                                                                                                   aaGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGGGCACGCAGCAACggggg                                                                                                                                           >  2:112557/1‑182 (MQ=255)
                                                                                                                    cgCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCAGCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCCGAAAAGACGTCTTTCGCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCTGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCgcgc                                                                                             <  1:710435/228‑1 (MQ=255)
                                                                                                                            cgcAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCa                                                                                                                                       <  2:421774/179‑1 (MQ=255)
                                                                                                                             gcAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGCAAAGACGTGTTTcgc                                                                                                                                                                                                                  <  2:662060/104‑1 (MQ=255)
                                                                                                                               aGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAATCGTTGCGCCAGGCTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTTCACGCAGCTACAGGTGGTCAAGGAGGCGCCGAGCCGACAGCGAGAGCGTGTGcg                                                                                                     <  2:398012/210‑2 (MQ=255)
                                                                                                                                     aCTTAATTGAGTGTTATGGTTGTCATCGCGGTCGCTCTGTCTGACGCGTTGAGCCAGACTAAGAGCGGGATGCCGGGCAGAAAAGACGTGTTTCGCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTg                                                                                                                                                           <  2:186230/150‑1 (MQ=255)
                                                                                                                                         cATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAgcg                                                                    <  1:212428/233‑1 (MQ=255)
                                                                                                                                                   tattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGC‑GCGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGt                                                                                                                                                            >  2:19550/1‑135 (MQ=255)
                                                                                                                                                       gttgtCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGtcagtcaga                                                   >  2:524096/1‑235 (MQ=255)
                                                                                                                                                             aTCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGGGCCGGGCGCGCATGCa                                                                                        >  1:137848/1‑193 (MQ=255)
                                                                                                                                                                gcgGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAACAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGGGCCgg                                                                                                   >  1:740163/1‑179 (MQ=255)
                                                                                                                                                                   gTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGCCAGAAAAGACGTGTTTCGCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGAc                                                                                  >  1:244197/1‑193 (MQ=255)
                                                                                                                                                                            tctgAGGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACa                                                                                                                    <  2:280911/150‑1 (MQ=255)
                                                                                                                                                                                aaGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGaaca                             <  2:400381/233‑1 (MQ=255)
                                                                                                                                                                                 aGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCaa                                                                       >  2:460954/1‑190 (MQ=255)
                                                                                                                                                                                   cGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGTCGAATTCACCaa                                                                       >  2:531084/1‑188 (MQ=255)
                                                                                                                                                                                        cgcCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAgcg                                                                    <  2:573612/186‑1 (MQ=255)
                                                                                                                                                                                          ccAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTGCGCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGaaca                             <  1:14046/223‑1 (MQ=255)
                                                                                                                                                                                           cAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAgtcgtc                                                          >  1:619098/1‑193 (MQ=255)
                                                                                                                                                                                               cTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAgc                                                                     <  2:285432/178‑1 (MQ=255)
                                                                                                                                                                                                     gCTGGATGACGGGCAGAAAAGACGTGTTTCGCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAACGCCGCATAGCGGGACACGGCct     >  1:317557/1‑236 (MQ=255)
                                                                                                                                                                                                           tGACGGGCAGAAAAGACGTGTTTCGCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACg                                                                                                                                                       <  2:354190/84‑1 (MQ=255)
                                                                                                                                                                                                           tGACGGGCAGAAAAGACGTGTTTCGCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACg                                                                                                                                                       >  1:354191/1‑84 (MQ=255)
                                                                                                                                                                                                           tGACGGGCAGAAAAGACGTGTTTCGCGG‑GCTGATCCAGCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAACGCCGCATAGCGGGACACGGCCTCtt  <  2:543262/233‑1 (MQ=255)
                                                                                                                                                                                                            gACGGGCAGAAAAGACGTGTTTCGCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAGCGCCGCATAGCgg               >  2:743446/1‑219 (MQ=255)
                                                                                                                                                                                                                                     |                                                                                                                                                                                                               
CAGCAAGCGTGCCGGCACGAACGACGCGACTTCCATATGGCGCACGCCGGCGCCGTAAGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGC‑G‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAACGCCGCATAGCGGGACACGGCCTCTT  >  AE015451/3845475‑3845909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑